Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle

Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle

Junping Wang,* Ceren Örnek-Ballanco,* Jiahua Xu, Weiguo Yang, Xiaojun YuDepartment of Chemistry, Chemical Biology, and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ, USA*These authors contributed equally to this workAbstract: Intracellular delivery vehicles have b...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Wang J, Örnek-Ballanco C, Xu J, Yang W, Yu X
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/1a744cde6cde432e9b33069bb3875167
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:1a744cde6cde432e9b33069bb3875167
record_format dspace
spelling oai:doaj.org-article:1a744cde6cde432e9b33069bb38751672021-12-02T05:07:12ZPreparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle1176-91141178-2013https://doaj.org/article/1a744cde6cde432e9b33069bb38751672012-12-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-vinculin-targeted-polymerndashlipi-a11834https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Junping Wang,* Ceren Örnek-Ballanco,* Jiahua Xu, Weiguo Yang, Xiaojun YuDepartment of Chemistry, Chemical Biology, and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ, USA*These authors contributed equally to this workAbstract: Intracellular delivery vehicles have been extensively investigated as these can serve as an effective tool in studying the cellular mechanism, by delivering functional protein to specific locations of the cells. In the current study, a polymer–lipid nanoparticle (PLN) system was developed as an intracellular delivery vehicle specifically targeting vinculin, a focal adhesion protein associated with cellular adhesive structures, such as focal adhesions and adherens junctions. The PLNs possessed an average size of 106 nm and had a positively charged surface. With a lower encapsulation efficiency 32% compared with poly(lactic-co-glycolic) acid (PLGA) nanoparticles (46%), the PLNs showed the sustained release profile of model drug BSA, while PLGA nanoparticles demonstrated an initial burst-release property. Cell-uptake experiments using mouse embryonic fibroblasts cultured in fibrin–fibronectin gels observed, under confocal microscope, that the anti-vinculin conjugated PLNs could successfully ship the cargo to the cytoplasm of fibroblasts, adhered to fibronectin–fibrin. With the use of cationic lipid, the unconjugated PLNs were shown to have high gene transfection efficiency. Furthermore, the unconjugated PLNs had nuclear-targeting capability in the absence of nuclear-localization signals. Therefore, the PLNs could be manipulated easily via different type of targeting ligands and could potentially be used as a powerful tool for cellular mechanism study, by delivering drugs to specific cellular organelles.Keywords: 3D gel, PLGA, targetingWang JÖrnek-Ballanco CXu JYang WYu XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 39-46 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Wang J
Örnek-Ballanco C
Xu J
Yang W
Yu X
Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
description Junping Wang,* Ceren Örnek-Ballanco,* Jiahua Xu, Weiguo Yang, Xiaojun YuDepartment of Chemistry, Chemical Biology, and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ, USA*These authors contributed equally to this workAbstract: Intracellular delivery vehicles have been extensively investigated as these can serve as an effective tool in studying the cellular mechanism, by delivering functional protein to specific locations of the cells. In the current study, a polymer–lipid nanoparticle (PLN) system was developed as an intracellular delivery vehicle specifically targeting vinculin, a focal adhesion protein associated with cellular adhesive structures, such as focal adhesions and adherens junctions. The PLNs possessed an average size of 106 nm and had a positively charged surface. With a lower encapsulation efficiency 32% compared with poly(lactic-co-glycolic) acid (PLGA) nanoparticles (46%), the PLNs showed the sustained release profile of model drug BSA, while PLGA nanoparticles demonstrated an initial burst-release property. Cell-uptake experiments using mouse embryonic fibroblasts cultured in fibrin–fibronectin gels observed, under confocal microscope, that the anti-vinculin conjugated PLNs could successfully ship the cargo to the cytoplasm of fibroblasts, adhered to fibronectin–fibrin. With the use of cationic lipid, the unconjugated PLNs were shown to have high gene transfection efficiency. Furthermore, the unconjugated PLNs had nuclear-targeting capability in the absence of nuclear-localization signals. Therefore, the PLNs could be manipulated easily via different type of targeting ligands and could potentially be used as a powerful tool for cellular mechanism study, by delivering drugs to specific cellular organelles.Keywords: 3D gel, PLGA, targeting
format article
author Wang J
Örnek-Ballanco C
Xu J
Yang W
Yu X
author_facet Wang J
Örnek-Ballanco C
Xu J
Yang W
Yu X
author_sort Wang J
title Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
title_short Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
title_full Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
title_fullStr Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
title_full_unstemmed Preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
title_sort preparation and characterization of vinculin-targeted polymer–lipid nanoparticle as intracellular delivery vehicle
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/1a744cde6cde432e9b33069bb3875167
work_keys_str_mv AT wangj preparationandcharacterizationofvinculintargetedpolymerampndashlipidnanoparticleasintracellulardeliveryvehicle
AT ampoumlrnekballancoc preparationandcharacterizationofvinculintargetedpolymerampndashlipidnanoparticleasintracellulardeliveryvehicle
AT xuj preparationandcharacterizationofvinculintargetedpolymerampndashlipidnanoparticleasintracellulardeliveryvehicle
AT yangw preparationandcharacterizationofvinculintargetedpolymerampndashlipidnanoparticleasintracellulardeliveryvehicle
AT yux preparationandcharacterizationofvinculintargetedpolymerampndashlipidnanoparticleasintracellulardeliveryvehicle
_version_ 1718400575410798592

Ejemplares similares