Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and its associated proteins (Cas) is an adaptive immune system in archaea and most bacteria. By repurposing these systems for use in eukaryote cells, a substantial revolution has arisen in the genome engineering field. In recent year...

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Autores principales:	Ali Dehshahri, Alessio Biagioni, Hadi Bayat, E. Hui Clarissa Lee, Mohammad Hashemabadi, Hojjat Samareh Fekri, Ali Zarrabi, Reza Mohammadinejad, Alan Prem Kumar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CRISPR SOX transcription factors gene editing cancer stem cells Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1a76d43697874765b6b113b83c7465e2
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id	oai:doaj.org-article:1a76d43697874765b6b113b83c7465e2
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spelling	oai:doaj.org-article:1a76d43697874765b6b113b83c7465e22021-11-11T16:48:29ZEditing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives10.3390/ijms2221113211422-00671661-6596https://doaj.org/article/1a76d43697874765b6b113b83c7465e22021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11321https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and its associated proteins (Cas) is an adaptive immune system in archaea and most bacteria. By repurposing these systems for use in eukaryote cells, a substantial revolution has arisen in the genome engineering field. In recent years, CRISPR-Cas technology was rapidly developed and different types of DNA or RNA sequence editors, gene activator or repressor, and epigenome modulators established. The versatility and feasibility of CRISPR-Cas technology has introduced this system as the most suitable tool for discovering and studying the mechanism of specific genes and also for generating appropriate cell and animal models. <i>SOX</i> genes play crucial roles in development processes and stemness. To elucidate the exact roles of SOX factors and their partners in tissue hemostasis and cell regeneration, generating appropriate in vitro and in vivo models is crucial. In line with these premises, CRISPR-Cas technology is a promising tool for studying different family members of SOX transcription factors. In this review, we aim to highlight the importance of CRISPR-Cas and summarize the applications of this novel, promising technology in studying and decoding the function of different members of the <i>SOX</i> gene family.Ali DehshahriAlessio BiagioniHadi BayatE. Hui Clarissa LeeMohammad HashemabadiHojjat Samareh FekriAli ZarrabiReza MohammadinejadAlan Prem KumarMDPI AGarticleCRISPRSOX transcription factorsgene editingcancerstem cellsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11321, p 11321 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR SOX transcription factors gene editing cancer stem cells Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	CRISPR SOX transcription factors gene editing cancer stem cells Biology (General) QH301-705.5 Chemistry QD1-999 Ali Dehshahri Alessio Biagioni Hadi Bayat E. Hui Clarissa Lee Mohammad Hashemabadi Hojjat Samareh Fekri Ali Zarrabi Reza Mohammadinejad Alan Prem Kumar Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
description	Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and its associated proteins (Cas) is an adaptive immune system in archaea and most bacteria. By repurposing these systems for use in eukaryote cells, a substantial revolution has arisen in the genome engineering field. In recent years, CRISPR-Cas technology was rapidly developed and different types of DNA or RNA sequence editors, gene activator or repressor, and epigenome modulators established. The versatility and feasibility of CRISPR-Cas technology has introduced this system as the most suitable tool for discovering and studying the mechanism of specific genes and also for generating appropriate cell and animal models. <i>SOX</i> genes play crucial roles in development processes and stemness. To elucidate the exact roles of SOX factors and their partners in tissue hemostasis and cell regeneration, generating appropriate in vitro and in vivo models is crucial. In line with these premises, CRISPR-Cas technology is a promising tool for studying different family members of SOX transcription factors. In this review, we aim to highlight the importance of CRISPR-Cas and summarize the applications of this novel, promising technology in studying and decoding the function of different members of the <i>SOX</i> gene family.
format	article
author	Ali Dehshahri Alessio Biagioni Hadi Bayat E. Hui Clarissa Lee Mohammad Hashemabadi Hojjat Samareh Fekri Ali Zarrabi Reza Mohammadinejad Alan Prem Kumar
author_facet	Ali Dehshahri Alessio Biagioni Hadi Bayat E. Hui Clarissa Lee Mohammad Hashemabadi Hojjat Samareh Fekri Ali Zarrabi Reza Mohammadinejad Alan Prem Kumar
author_sort	Ali Dehshahri
title	Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
title_short	Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
title_full	Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
title_fullStr	Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
title_full_unstemmed	Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives
title_sort	editing <i>sox</i> genes by crispr-cas: current insights and future perspectives
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a76d43697874765b6b113b83c7465e2
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Editing <i>SOX</i> Genes by CRISPR-Cas: Current Insights and Future Perspectives

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