Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

ABSTRACT Background: Malaria, caused by Plasmodium sp infection, is a major global cause of morbidity and mortality. Most experimental cerebral malaria (ECM) studies show increase number of Th1 cells and CTLs in the brain, due to increase chemokine expression, including CXCL10, a potent chemokine i...

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Autores principales:	Abdulhakim Sulayman, Kis - Djamiatun, Muflihatul Muniroh
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2019
Materias:	annona muricata artemisinin p. berghei anka cxcl10 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1a79097eb2924fb8a2db8430dac40daf
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spelling	oai:doaj.org-article:1a79097eb2924fb8a2db8430dac40daf2021-11-05T16:47:32ZEffectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)2503-217810.14710/jbtr.v5i2.4802https://doaj.org/article/1a79097eb2924fb8a2db8430dac40daf2019-12-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/4802https://doaj.org/toc/2503-2178ABSTRACT Background: Malaria, caused by Plasmodium sp infection, is a major global cause of morbidity and mortality. Most experimental cerebral malaria (ECM) studies show increase number of Th1 cells and CTLs in the brain, due to increase chemokine expression, including CXCL10, a potent chemokine involved in cerebral malaria (CM). Recent studies show that CXCL10 provokes apoptosis of human brain micro-endothelial cells and in vitro neuroglia cells. Objective: To determine whether combination of Annona muricata-leaf-extracted-by-water (AME) and artemisinin-combination-therapy (ACT) reduce brain-CXCL10-expression of Swiss-mice inoculated with P. berghei ANKA (PbA). Methods: This was an experimental-study with post-test-only-control-group-design. Twenty-four Swiss-mice (PbA-inoculated) were randomly divided into 4 groups. Control group (C) was PbA inoculated only. X1, X2 and X3 groups received AME, ACT and combination of AME and ACT treatment, respectively. CXCL10 was stained with in immunohistochemistry, which then observed by light microscope in order to determine Allred-score. Kruskal-Wallis test was used to statistically analyze the differences among groups, then followed by a Mann- Whitney U test. Result: C and X1groups had severe-PbA-infection when the study was end on day-7-PbA-infection, while X2 and X3 groups entered recovery-stage. The AME-ACT-treatment-group had significantly lower of brain-CXCL10-expression than AME-group (p=0.008) and nearly significantly lower than control-group (p=0.058). Group that received ACT alone had no different value of brain-CXCL10-expression than control-group (p=0.502) and combination AME–ACT group (p=0.335). Conclusion: The combination of AME–ACT treatment decreases brain-CXCL10-expression of Swiss-mice during PbA-infection-recovery-stage, indicating the effectivity of AME–ACT combined therapy is better prevention of cerebral malaria than AME alone.Abdulhakim SulaymanKis - DjamiatunMuflihatul MunirohDiponegoro Universityarticleannona muricataartemisininp. berghei ankacxcl10Medicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 5, Iss 2, Pp 47-52 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	annona muricata artemisinin p. berghei anka cxcl10 Medicine (General) R5-920
spellingShingle	annona muricata artemisinin p. berghei anka cxcl10 Medicine (General) R5-920 Abdulhakim Sulayman Kis - Djamiatun Muflihatul Muniroh Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
description	ABSTRACT Background: Malaria, caused by Plasmodium sp infection, is a major global cause of morbidity and mortality. Most experimental cerebral malaria (ECM) studies show increase number of Th1 cells and CTLs in the brain, due to increase chemokine expression, including CXCL10, a potent chemokine involved in cerebral malaria (CM). Recent studies show that CXCL10 provokes apoptosis of human brain micro-endothelial cells and in vitro neuroglia cells. Objective: To determine whether combination of Annona muricata-leaf-extracted-by-water (AME) and artemisinin-combination-therapy (ACT) reduce brain-CXCL10-expression of Swiss-mice inoculated with P. berghei ANKA (PbA). Methods: This was an experimental-study with post-test-only-control-group-design. Twenty-four Swiss-mice (PbA-inoculated) were randomly divided into 4 groups. Control group (C) was PbA inoculated only. X1, X2 and X3 groups received AME, ACT and combination of AME and ACT treatment, respectively. CXCL10 was stained with in immunohistochemistry, which then observed by light microscope in order to determine Allred-score. Kruskal-Wallis test was used to statistically analyze the differences among groups, then followed by a Mann- Whitney U test. Result: C and X1groups had severe-PbA-infection when the study was end on day-7-PbA-infection, while X2 and X3 groups entered recovery-stage. The AME-ACT-treatment-group had significantly lower of brain-CXCL10-expression than AME-group (p=0.008) and nearly significantly lower than control-group (p=0.058). Group that received ACT alone had no different value of brain-CXCL10-expression than control-group (p=0.502) and combination AME–ACT group (p=0.335). Conclusion: The combination of AME–ACT treatment decreases brain-CXCL10-expression of Swiss-mice during PbA-infection-recovery-stage, indicating the effectivity of AME–ACT combined therapy is better prevention of cerebral malaria than AME alone.
format	article
author	Abdulhakim Sulayman Kis - Djamiatun Muflihatul Muniroh
author_facet	Abdulhakim Sulayman Kis - Djamiatun Muflihatul Muniroh
author_sort	Abdulhakim Sulayman
title	Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
title_short	Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
title_full	Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
title_fullStr	Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
title_full_unstemmed	Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)
title_sort	effectivity of annona muricata and artemisinin combined therapy on brain cxcl10 expression (study in swiss mice during severe plasmodium berghei anka infection)
publisher	Diponegoro University
publishDate	2019
url	https://doaj.org/article/1a79097eb2924fb8a2db8430dac40daf
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Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

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