Bacteria and methanogen community in the rumen fed different levels of grass-legume silages

Abstract. Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, Sakamoto M, Ohkuma M. 2019. Bacteria and methanogen community in the rumen fed different levels of grass-legume silages. Biodiversitas 20: 1055-1062. This study aimed to investigate the effects of dietary grass-legume silages on t...

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Autores principales:	RONI RIDWAN, IMAN RUSMANA, YANTYATI WIDYASTUTI, KOMANG G. WIRYAWAN, BAMBANG PRASETYA, MITSUO SAKAMOTO, MORIYA OHKUMA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	grass-legumes silage, culture-independent, microbial community, 16s rdna sequence Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1a7a0e8232bc46f08b466abfd66441c4
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spelling	oai:doaj.org-article:1a7a0e8232bc46f08b466abfd66441c42021-11-21T21:40:39ZBacteria and methanogen community in the rumen fed different levels of grass-legume silages1412-033X2085-472210.13057/biodiv/d200417https://doaj.org/article/1a7a0e8232bc46f08b466abfd66441c42019-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3344https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, Sakamoto M, Ohkuma M. 2019. Bacteria and methanogen community in the rumen fed different levels of grass-legume silages. Biodiversitas 20: 1055-1062. This study aimed to investigate the effects of dietary grass-legume silages on the microbial community by using a culture-independent approach. Treatments consisted of R0: 50% Pennisetum purpureum and 50 % concentrate; R1: 20% P. purpureum, 50 % concentrate, and 30% grass-legumes silage; R2: 20% P. purpureum, 35 % concentrate, and 45% grass-legumes silage; and R3; 20% P. purpureum, 20 % concentrate, and 60% grass-legumes silage. The rumen fluid obtained from fistulated cattle was used for T-RFLP, 16S rDNA clone library, and qPCR analyses. The results indicated that bacterial diversity was dominated by Bacteroidetes, Firmicutes, and methanogen by Methanobacteriales, based on partial 16S rDNA sequences. The microbial communities were dominated by Prevotella brevis, P. ruminicola, Succiniclasticum ruminis, and Methanobrevibacter ruminantium, M. smithi, M. thueri, and M. millerae. The increasing silage diet in a rumen suppressed methanogenesis by reducing population distribution of Methanobacteriales, directly or indirectly, by reducing the diversity of bacterial populations. Generally, the increase silage in the diet changed the bacterial and methanogen community. Grass-legume silage diets of less than 45% are potential for ruminant diet to reduce methane production by a decrease of 4% in the relative distribution of methanogens in the rumen.RONI RIDWANIMAN RUSMANAYANTYATI WIDYASTUTIKOMANG G. WIRYAWANBAMBANG PRASETYAMITSUO SAKAMOTOMORIYA OHKUMAMBI & UNS Soloarticlegrass-legumes silage, culture-independent, microbial community, 16s rdna sequenceBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 4, Pp 1055-1062 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	grass-legumes silage, culture-independent, microbial community, 16s rdna sequence Biology (General) QH301-705.5
spellingShingle	grass-legumes silage, culture-independent, microbial community, 16s rdna sequence Biology (General) QH301-705.5 RONI RIDWAN IMAN RUSMANA YANTYATI WIDYASTUTI KOMANG G. WIRYAWAN BAMBANG PRASETYA MITSUO SAKAMOTO MORIYA OHKUMA Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
description	Abstract. Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, Sakamoto M, Ohkuma M. 2019. Bacteria and methanogen community in the rumen fed different levels of grass-legume silages. Biodiversitas 20: 1055-1062. This study aimed to investigate the effects of dietary grass-legume silages on the microbial community by using a culture-independent approach. Treatments consisted of R0: 50% Pennisetum purpureum and 50 % concentrate; R1: 20% P. purpureum, 50 % concentrate, and 30% grass-legumes silage; R2: 20% P. purpureum, 35 % concentrate, and 45% grass-legumes silage; and R3; 20% P. purpureum, 20 % concentrate, and 60% grass-legumes silage. The rumen fluid obtained from fistulated cattle was used for T-RFLP, 16S rDNA clone library, and qPCR analyses. The results indicated that bacterial diversity was dominated by Bacteroidetes, Firmicutes, and methanogen by Methanobacteriales, based on partial 16S rDNA sequences. The microbial communities were dominated by Prevotella brevis, P. ruminicola, Succiniclasticum ruminis, and Methanobrevibacter ruminantium, M. smithi, M. thueri, and M. millerae. The increasing silage diet in a rumen suppressed methanogenesis by reducing population distribution of Methanobacteriales, directly or indirectly, by reducing the diversity of bacterial populations. Generally, the increase silage in the diet changed the bacterial and methanogen community. Grass-legume silage diets of less than 45% are potential for ruminant diet to reduce methane production by a decrease of 4% in the relative distribution of methanogens in the rumen.
format	article
author	RONI RIDWAN IMAN RUSMANA YANTYATI WIDYASTUTI KOMANG G. WIRYAWAN BAMBANG PRASETYA MITSUO SAKAMOTO MORIYA OHKUMA
author_facet	RONI RIDWAN IMAN RUSMANA YANTYATI WIDYASTUTI KOMANG G. WIRYAWAN BAMBANG PRASETYA MITSUO SAKAMOTO MORIYA OHKUMA
author_sort	RONI RIDWAN
title	Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
title_short	Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
title_full	Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
title_fullStr	Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
title_full_unstemmed	Bacteria and methanogen community in the rumen fed different levels of grass-legume silages
title_sort	bacteria and methanogen community in the rumen fed different levels of grass-legume silages
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/1a7a0e8232bc46f08b466abfd66441c4
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Bacteria and methanogen community in the rumen fed different levels of grass-legume silages

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