Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds

Abstract Salmonella is one of the most hazardous diseases in poultry farms. Markedly, the application of active immunostimulants is illustrated as potential protective agents against infection in poultry farms. Thus, this work aimed to explore inter‐ and intra‐breed variation in response to acute an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El‐Nahas, Walaa S.H. Abd El Naby, Sabry Hassan, Jamal A. Alorabi, Mahmoud A.O. Dawood
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	breed effect gene expression immune response immunomodulator Salmonella enteritidis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/1a7a2f7fed9b4d3ebd425116009ebf61
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1a7a2f7fed9b4d3ebd425116009ebf61
record_format	dspace
spelling	oai:doaj.org-article:1a7a2f7fed9b4d3ebd425116009ebf612021-11-19T17:14:25ZIntra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds2053-109510.1002/vms3.621https://doaj.org/article/1a7a2f7fed9b4d3ebd425116009ebf612021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.621https://doaj.org/toc/2053-1095Abstract Salmonella is one of the most hazardous diseases in poultry farms. Markedly, the application of active immunostimulants is illustrated as potential protective agents against infection in poultry farms. Thus, this work aimed to explore inter‐ and intra‐breed variation in response to acute and subchronic Salmonella enteritidis infection in two‐layer breeds (one commercial [Hy‐line strain] and another native [Fayoumi breed]). Besides exploring the possible protective effect of a commercial immune modulator (STIMULAN) on the two breeds during the acute infection. The ELISA antibody titer in sub‐chronic infections and the expression analysis of some selected genes (IL‐1β, LITAF, TGF‐β, HSP90 and HSP70) are used as the clinical signs for acute infections to assess the possible protective role of a commercial immunomodulator (STIMULAN). Five groups were used during the acute experiment: G1—control; G2a—susceptible; G2b—resistant birds, G3—which received STIMULAN and G4—which received the infection + STIMULAN. The groups with sub‐chronic infections include G1 (control), G2 (high antibody titer) and G3 (low antibody titer). The gene expressions among the susceptible birds during acute infection of both breeds are nearly similar. They only differ in the expression of HSP90 in the Fayoumi breed. However, the resistant birds vary in their gene expression profile. The effect of STIMULAN as a feed additive in non‐infected birds was an up‐regulation of LITAF, TGF‐β, HSP90 in Fayoumi. Moreover, a powerful stimulatory role was observed when both breeds were infected. Both breeds were asymptomatic during the sub‐chronic infection. Although, the increased expression of inflammatory‐related genes in the Hy‐line was considered as an indication of infection persistence. Fayoumi is capable of immune clearance for this infection. Thus, the Fayoumi breed is more resistant to acute Salmonella infection. HSP90 plays a vital role in its resistance. We recommend the use of STIMULAN as an immunomodulator during Salmonella infection.Shymaa A. KhatabShabaan A. HemedaAbeer F. El‐NahasWalaa S.H. Abd El NabySabry HassanJamal A. AlorabiMahmoud A.O. DawoodWileyarticlebreed effectgene expressionimmune responseimmunomodulatorSalmonella enteritidisVeterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2353-2361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breed effect gene expression immune response immunomodulator Salmonella enteritidis Veterinary medicine SF600-1100
spellingShingle	breed effect gene expression immune response immunomodulator Salmonella enteritidis Veterinary medicine SF600-1100 Shymaa A. Khatab Shabaan A. Hemeda Abeer F. El‐Nahas Walaa S.H. Abd El Naby Sabry Hassan Jamal A. Alorabi Mahmoud A.O. Dawood Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
description	Abstract Salmonella is one of the most hazardous diseases in poultry farms. Markedly, the application of active immunostimulants is illustrated as potential protective agents against infection in poultry farms. Thus, this work aimed to explore inter‐ and intra‐breed variation in response to acute and subchronic Salmonella enteritidis infection in two‐layer breeds (one commercial [Hy‐line strain] and another native [Fayoumi breed]). Besides exploring the possible protective effect of a commercial immune modulator (STIMULAN) on the two breeds during the acute infection. The ELISA antibody titer in sub‐chronic infections and the expression analysis of some selected genes (IL‐1β, LITAF, TGF‐β, HSP90 and HSP70) are used as the clinical signs for acute infections to assess the possible protective role of a commercial immunomodulator (STIMULAN). Five groups were used during the acute experiment: G1—control; G2a—susceptible; G2b—resistant birds, G3—which received STIMULAN and G4—which received the infection + STIMULAN. The groups with sub‐chronic infections include G1 (control), G2 (high antibody titer) and G3 (low antibody titer). The gene expressions among the susceptible birds during acute infection of both breeds are nearly similar. They only differ in the expression of HSP90 in the Fayoumi breed. However, the resistant birds vary in their gene expression profile. The effect of STIMULAN as a feed additive in non‐infected birds was an up‐regulation of LITAF, TGF‐β, HSP90 in Fayoumi. Moreover, a powerful stimulatory role was observed when both breeds were infected. Both breeds were asymptomatic during the sub‐chronic infection. Although, the increased expression of inflammatory‐related genes in the Hy‐line was considered as an indication of infection persistence. Fayoumi is capable of immune clearance for this infection. Thus, the Fayoumi breed is more resistant to acute Salmonella infection. HSP90 plays a vital role in its resistance. We recommend the use of STIMULAN as an immunomodulator during Salmonella infection.
format	article
author	Shymaa A. Khatab Shabaan A. Hemeda Abeer F. El‐Nahas Walaa S.H. Abd El Naby Sabry Hassan Jamal A. Alorabi Mahmoud A.O. Dawood
author_facet	Shymaa A. Khatab Shabaan A. Hemeda Abeer F. El‐Nahas Walaa S.H. Abd El Naby Sabry Hassan Jamal A. Alorabi Mahmoud A.O. Dawood
author_sort	Shymaa A. Khatab
title	Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
title_short	Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
title_full	Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
title_fullStr	Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
title_full_unstemmed	Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds
title_sort	intra‐ and inter‐breed variation in immune response to acute and sub‐chronic salmonella infection and commercial immune‐stimulant in two‐layer breeds
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/1a7a2f7fed9b4d3ebd425116009ebf61
work_keys_str_mv	AT shymaaakhatab intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT shabaanahemeda intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT abeerfelnahas intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT walaashabdelnaby intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT sabryhassan intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT jamalaalorabi intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds AT mahmoudaodawood intraandinterbreedvariationinimmuneresponsetoacuteandsubchronicsalmonellainfectionandcommercialimmunestimulantintwolayerbreeds
_version_	1718419997553852416

Intra‐ and inter‐breed variation in immune response to acute and sub‐chronic Salmonella infection and commercial immune‐stimulant in two‐layer breeds

Ejemplares similares