Blue-light reception through quaternary transitions

Abstract Sensory photoreceptors absorb light via their photosensor modules and trigger downstream physiological adaptations via their effector modules. Light reception accordingly depends on precisely orchestrated interactions between these modules, the molecular details of which often remain elusiv...

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Autores principales:	Christopher Engelhard, Ralph P. Diensthuber, Andreas Möglich, Robert Bittl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a7b4e17c7594dd4ad5fcd343c1a8905
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spelling	oai:doaj.org-article:1a7b4e17c7594dd4ad5fcd343c1a89052021-12-02T12:32:58ZBlue-light reception through quaternary transitions10.1038/s41598-017-01497-72045-2322https://doaj.org/article/1a7b4e17c7594dd4ad5fcd343c1a89052017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01497-7https://doaj.org/toc/2045-2322Abstract Sensory photoreceptors absorb light via their photosensor modules and trigger downstream physiological adaptations via their effector modules. Light reception accordingly depends on precisely orchestrated interactions between these modules, the molecular details of which often remain elusive. Using electron-electron double resonance (ELDOR) spectroscopy and site-directed spin labelling, we chart the structural transitions facilitating blue-light reception in the engineered light-oxygen-voltage (LOV) histidine kinase YF1 which represents a paradigm for numerous natural signal receptors. Structural modelling based on pair-wise distance constraints derived from ELDOR pinpoint light-induced rotation and splaying apart of the two LOV photosensors in the dimeric photoreceptor. Resultant molecular strain likely relaxes as left-handed supercoiling of the coiled-coil linker connecting sensor and effector units. ELDOR data on a photoreceptor variant with an inverted signal response indicate a drastically altered dimer interface but light-induced structural transitions in the linker that are similar to those in YF1. Taken together, we provide mechanistic insight into the signal trajectories of LOV photoreceptors and histidine kinases that inform molecular simulations and the engineering of novel receptors.Christopher EngelhardRalph P. DiensthuberAndreas MöglichRobert BittlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher Engelhard Ralph P. Diensthuber Andreas Möglich Robert Bittl Blue-light reception through quaternary transitions
description	Abstract Sensory photoreceptors absorb light via their photosensor modules and trigger downstream physiological adaptations via their effector modules. Light reception accordingly depends on precisely orchestrated interactions between these modules, the molecular details of which often remain elusive. Using electron-electron double resonance (ELDOR) spectroscopy and site-directed spin labelling, we chart the structural transitions facilitating blue-light reception in the engineered light-oxygen-voltage (LOV) histidine kinase YF1 which represents a paradigm for numerous natural signal receptors. Structural modelling based on pair-wise distance constraints derived from ELDOR pinpoint light-induced rotation and splaying apart of the two LOV photosensors in the dimeric photoreceptor. Resultant molecular strain likely relaxes as left-handed supercoiling of the coiled-coil linker connecting sensor and effector units. ELDOR data on a photoreceptor variant with an inverted signal response indicate a drastically altered dimer interface but light-induced structural transitions in the linker that are similar to those in YF1. Taken together, we provide mechanistic insight into the signal trajectories of LOV photoreceptors and histidine kinases that inform molecular simulations and the engineering of novel receptors.
format	article
author	Christopher Engelhard Ralph P. Diensthuber Andreas Möglich Robert Bittl
author_facet	Christopher Engelhard Ralph P. Diensthuber Andreas Möglich Robert Bittl
author_sort	Christopher Engelhard
title	Blue-light reception through quaternary transitions
title_short	Blue-light reception through quaternary transitions
title_full	Blue-light reception through quaternary transitions
title_fullStr	Blue-light reception through quaternary transitions
title_full_unstemmed	Blue-light reception through quaternary transitions
title_sort	blue-light reception through quaternary transitions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1a7b4e17c7594dd4ad5fcd343c1a8905
work_keys_str_mv	AT christopherengelhard bluelightreceptionthroughquaternarytransitions AT ralphpdiensthuber bluelightreceptionthroughquaternarytransitions AT andreasmoglich bluelightreceptionthroughquaternarytransitions AT robertbittl bluelightreceptionthroughquaternarytransitions
_version_	1718393904573710336

Blue-light reception through quaternary transitions

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