Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.

<h4>Aims</h4>The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin...

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Autores principales:	Aurigena Antunes de Araújo, Hugo Varela, Gerly Anne de Castro Brito, Caroline Addison Carvalho Xavier de Medeiros, Lorena de Souza Araújo, José Heriberto Oliveira do Nascimento, Raimundo Fernandes de Araújo Júnior
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a7d800fce3a4ba397ec52b701f69b19
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spelling	oai:doaj.org-article:1a7d800fce3a4ba397ec52b701f69b192021-11-18T08:19:43ZAzilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.1932-620310.1371/journal.pone.0096750https://doaj.org/article/1a7d800fce3a4ba397ec52b701f69b192014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24819928/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aims</h4>The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal tissue in a rat model of ligature-induced periodontitis.<h4>Materials and methods</h4>Male Wistar albino rats were randomly divided into 5 groups of 10 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1 mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were treated with saline or AZT for 10 days. Periodontal tissues were analyzed by histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2, RANKL, RANK, OPG, and cathepsin K. Levels of IL-1β, IL-10, TNF-α, myeloperoxidase (MPO), and glutathione (GSH) were determined by ELISA.<h4>Results</h4>Treatment with 5 mg/kg AZT resulted in reduced MPO (p<0.05) and IL-1β (p<0.05), increased levels of IL-10 (p<0.05), and reduced expression of MMP-2, MMP-9, COX-2, RANK, RANKL, cathepsin K, and increased expression of OPG.<h4>Conclusions</h4>These findings reveal that AZT increases anti-inflammatory cytokines and GSH and decreases bone loss in ligature-induced periodontitis in rats.Aurigena Antunes de AraújoHugo VarelaGerly Anne de Castro BritoCaroline Addison Carvalho Xavier de MedeirosLorena de Souza AraújoJosé Heriberto Oliveira do NascimentoRaimundo Fernandes de Araújo JúniorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96750 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aurigena Antunes de Araújo Hugo Varela Gerly Anne de Castro Brito Caroline Addison Carvalho Xavier de Medeiros Lorena de Souza Araújo José Heriberto Oliveira do Nascimento Raimundo Fernandes de Araújo Júnior Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
description	<h4>Aims</h4>The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal tissue in a rat model of ligature-induced periodontitis.<h4>Materials and methods</h4>Male Wistar albino rats were randomly divided into 5 groups of 10 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1 mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were treated with saline or AZT for 10 days. Periodontal tissues were analyzed by histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2, RANKL, RANK, OPG, and cathepsin K. Levels of IL-1β, IL-10, TNF-α, myeloperoxidase (MPO), and glutathione (GSH) were determined by ELISA.<h4>Results</h4>Treatment with 5 mg/kg AZT resulted in reduced MPO (p<0.05) and IL-1β (p<0.05), increased levels of IL-10 (p<0.05), and reduced expression of MMP-2, MMP-9, COX-2, RANK, RANKL, cathepsin K, and increased expression of OPG.<h4>Conclusions</h4>These findings reveal that AZT increases anti-inflammatory cytokines and GSH and decreases bone loss in ligature-induced periodontitis in rats.
format	article
author	Aurigena Antunes de Araújo Hugo Varela Gerly Anne de Castro Brito Caroline Addison Carvalho Xavier de Medeiros Lorena de Souza Araújo José Heriberto Oliveira do Nascimento Raimundo Fernandes de Araújo Júnior
author_facet	Aurigena Antunes de Araújo Hugo Varela Gerly Anne de Castro Brito Caroline Addison Carvalho Xavier de Medeiros Lorena de Souza Araújo José Heriberto Oliveira do Nascimento Raimundo Fernandes de Araújo Júnior
author_sort	Aurigena Antunes de Araújo
title	Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
title_short	Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
title_full	Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
title_fullStr	Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
title_full_unstemmed	Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.
title_sort	azilsartan increases levels of il-10, down-regulates mmp-2, mmp-9, rankl/rank, cathepsin k and up-regulates opg in an experimental periodontitis model.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1a7d800fce3a4ba397ec52b701f69b19
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Azilsartan increases levels of IL-10, down-regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and up-regulates OPG in an experimental periodontitis model.

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