Dissecting rice polyamine metabolism under controlled long-term drought stress.

A selection of 21 rice cultivars (Oryza sativa L. ssp. indica and japonica) was characterized under moderate long-term drought stress by comprehensive physiological analyses and determination of the contents of polyamines and selected metabolites directly related to polyamine metabolism. To investig...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Phuc Thi Do, Thomas Degenkolbe, Alexander Erban, Arnd G Heyer, Joachim Kopka, Karin I Köhl, Dirk K Hincha, Ellen Zuther
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a7fd243e6d4424b830dc5e9afa01e01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1a7fd243e6d4424b830dc5e9afa01e01
record_format	dspace
spelling	oai:doaj.org-article:1a7fd243e6d4424b830dc5e9afa01e012021-11-18T07:50:20ZDissecting rice polyamine metabolism under controlled long-term drought stress.1932-620310.1371/journal.pone.0060325https://doaj.org/article/1a7fd243e6d4424b830dc5e9afa01e012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23577102/?tool=EBIhttps://doaj.org/toc/1932-6203A selection of 21 rice cultivars (Oryza sativa L. ssp. indica and japonica) was characterized under moderate long-term drought stress by comprehensive physiological analyses and determination of the contents of polyamines and selected metabolites directly related to polyamine metabolism. To investigate the potential regulation of polyamine biosynthesis at the transcriptional level, the expression of 21 genes encoding enzymes involved in these pathways were analyzed by qRT-PCR. Analysis of the genomic loci revealed that 11 of these genes were located in drought-related QTL regions, in agreement with a proposed role of polyamine metabolism in rice drought tolerance. The cultivars differed widely in their drought tolerance and parameters such as biomass and photosynthetic quantum yield were significantly affected by drought treatment. Under optimal irrigation free putrescine was the predominant polyamine followed by free spermidine and spermine. When exposed to drought putrescine levels decreased markedly and spermine became predominant in all cultivars. There were no correlations between polyamine contents and drought tolerance. GC-MS analysis revealed drought-induced changes of the levels of ornithine/arginine (substrate), substrates of polyamine synthesis, proline, product of a competing pathway and GABA, a potential degradation product. Gene expression analysis indicated that ADC-dependent polyamine biosynthesis responded much more strongly to drought than the ODC-dependent pathway. Nevertheless the fold change in transcript abundance of ODC1 under drought stress was linearly correlated with the drought tolerance of the cultivars. Combining metabolite and gene expression data, we propose a model of the coordinate adjustment of polyamine biosynthesis for the accumulation of spermine under drought conditions.Phuc Thi DoThomas DegenkolbeAlexander ErbanArnd G HeyerJoachim KopkaKarin I KöhlDirk K HinchaEllen ZutherPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60325 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Phuc Thi Do Thomas Degenkolbe Alexander Erban Arnd G Heyer Joachim Kopka Karin I Köhl Dirk K Hincha Ellen Zuther Dissecting rice polyamine metabolism under controlled long-term drought stress.
description	A selection of 21 rice cultivars (Oryza sativa L. ssp. indica and japonica) was characterized under moderate long-term drought stress by comprehensive physiological analyses and determination of the contents of polyamines and selected metabolites directly related to polyamine metabolism. To investigate the potential regulation of polyamine biosynthesis at the transcriptional level, the expression of 21 genes encoding enzymes involved in these pathways were analyzed by qRT-PCR. Analysis of the genomic loci revealed that 11 of these genes were located in drought-related QTL regions, in agreement with a proposed role of polyamine metabolism in rice drought tolerance. The cultivars differed widely in their drought tolerance and parameters such as biomass and photosynthetic quantum yield were significantly affected by drought treatment. Under optimal irrigation free putrescine was the predominant polyamine followed by free spermidine and spermine. When exposed to drought putrescine levels decreased markedly and spermine became predominant in all cultivars. There were no correlations between polyamine contents and drought tolerance. GC-MS analysis revealed drought-induced changes of the levels of ornithine/arginine (substrate), substrates of polyamine synthesis, proline, product of a competing pathway and GABA, a potential degradation product. Gene expression analysis indicated that ADC-dependent polyamine biosynthesis responded much more strongly to drought than the ODC-dependent pathway. Nevertheless the fold change in transcript abundance of ODC1 under drought stress was linearly correlated with the drought tolerance of the cultivars. Combining metabolite and gene expression data, we propose a model of the coordinate adjustment of polyamine biosynthesis for the accumulation of spermine under drought conditions.
format	article
author	Phuc Thi Do Thomas Degenkolbe Alexander Erban Arnd G Heyer Joachim Kopka Karin I Köhl Dirk K Hincha Ellen Zuther
author_facet	Phuc Thi Do Thomas Degenkolbe Alexander Erban Arnd G Heyer Joachim Kopka Karin I Köhl Dirk K Hincha Ellen Zuther
author_sort	Phuc Thi Do
title	Dissecting rice polyamine metabolism under controlled long-term drought stress.
title_short	Dissecting rice polyamine metabolism under controlled long-term drought stress.
title_full	Dissecting rice polyamine metabolism under controlled long-term drought stress.
title_fullStr	Dissecting rice polyamine metabolism under controlled long-term drought stress.
title_full_unstemmed	Dissecting rice polyamine metabolism under controlled long-term drought stress.
title_sort	dissecting rice polyamine metabolism under controlled long-term drought stress.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1a7fd243e6d4424b830dc5e9afa01e01
work_keys_str_mv	AT phucthido dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT thomasdegenkolbe dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT alexandererban dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT arndgheyer dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT joachimkopka dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT karinikohl dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT dirkkhincha dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress AT ellenzuther dissectingricepolyaminemetabolismundercontrolledlongtermdroughtstress
_version_	1718422837986852864

Dissecting rice polyamine metabolism under controlled long-term drought stress.

Ejemplares similares