In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles

Lei Shi,1 Xiuli Wang,1 Feng Zhao,2 Hansen Luan,2 Qingfeng Tu,1 Zheng Huang,3 Hao Wang,2 Hongwei Wang1,41Shanghai Skin Disease Hospital, Shanghai, People's Republic of China; 2National Pharmaceutical Engineering Research Center, China State Institute of Pharmaceutical Industry, Shanghai, Peop...

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Autores principales:	Shi L, Wang XL, Zhao F, Luan HS, Tu QF, Huang Z, Wang H, Wang HW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1a854943b04a480e998532c164b44b09
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spelling	oai:doaj.org-article:1a854943b04a480e998532c164b44b092021-12-02T06:59:25ZIn vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles1176-91141178-2013https://doaj.org/article/1a854943b04a480e998532c164b44b092013-07-01T00:00:00Zhttp://www.dovepress.com/in-vitro-evaluation-of-5-aminolevulinic-acid-ala-loaded-plga-nanoparti-a13779https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lei Shi,1 Xiuli Wang,1 Feng Zhao,2 Hansen Luan,2 Qingfeng Tu,1 Zheng Huang,3 Hao Wang,2 Hongwei Wang1,41Shanghai Skin Disease Hospital, Shanghai, People's Republic of China; 2National Pharmaceutical Engineering Research Center, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China; 3Ministry of Education (MOE) Key Laboratory of OptoElectronic Science and Technology for Medicine, Fujian Normal University, Fuzhou, People's Republic of China; 4Huadong Hospital, Fudan University, Shanghai, People's Republic of ChinaBackground: 5-Aminolevulinic acid (ALA) is a prodrug for topical photodynamic therapy. The effectiveness of topical ALA can be limited by its bioavailability. The aim of this study was to develop a novel ALA delivery approach using poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs).Methods: A modified double emulsion solvent evaporation method was used to prepare ALA loaded PLGA NPs (ALA PLGA NPs). The characteristics, uptake, protoporphyrin IX fluorescence kinetics, and cytotoxicity of ALA PLGA NPs toward a human skin squamous cell carcinoma cell line were examined.Results: The mean particle size of spherical ALA PLGA NPs was 65.6 nm ± 26 nm with a polydispersity index of 0.62. The encapsulation efficiency was 65.8% ± 7.2% and ALA loading capacity was 0.62% ± 0.27%. When ALA was dispersed in PLGA NPs, it turned into an amorphous phase. ALA PLGA NPs could be taken up by squamous cell carcinoma cells and localized in the cytoplasm. The protoporphyrin IX fluorescence kinetics and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay showed that ALA PLGA NPs were more effective than free ALA of the same concentration.Conclusion: PLGA NPs provide a promising ALA delivery strategy for topical ALA-photodynamic therapy of skin squamous cell carcinoma.Keywords: 5-Aminolevulinic acid (ALA), nanoparticles, poly(lactic-co-glycolic acid) (PLGA), skin squamous cell carcinoma, photodynamic therapy (PDT)Shi LWang XLZhao FLuan HSTu QFHuang ZWang HWang HWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2669-2676 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shi L Wang XL Zhao F Luan HS Tu QF Huang Z Wang H Wang HW In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
description	Lei Shi,1 Xiuli Wang,1 Feng Zhao,2 Hansen Luan,2 Qingfeng Tu,1 Zheng Huang,3 Hao Wang,2 Hongwei Wang1,41Shanghai Skin Disease Hospital, Shanghai, People's Republic of China; 2National Pharmaceutical Engineering Research Center, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China; 3Ministry of Education (MOE) Key Laboratory of OptoElectronic Science and Technology for Medicine, Fujian Normal University, Fuzhou, People's Republic of China; 4Huadong Hospital, Fudan University, Shanghai, People's Republic of ChinaBackground: 5-Aminolevulinic acid (ALA) is a prodrug for topical photodynamic therapy. The effectiveness of topical ALA can be limited by its bioavailability. The aim of this study was to develop a novel ALA delivery approach using poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs).Methods: A modified double emulsion solvent evaporation method was used to prepare ALA loaded PLGA NPs (ALA PLGA NPs). The characteristics, uptake, protoporphyrin IX fluorescence kinetics, and cytotoxicity of ALA PLGA NPs toward a human skin squamous cell carcinoma cell line were examined.Results: The mean particle size of spherical ALA PLGA NPs was 65.6 nm ± 26 nm with a polydispersity index of 0.62. The encapsulation efficiency was 65.8% ± 7.2% and ALA loading capacity was 0.62% ± 0.27%. When ALA was dispersed in PLGA NPs, it turned into an amorphous phase. ALA PLGA NPs could be taken up by squamous cell carcinoma cells and localized in the cytoplasm. The protoporphyrin IX fluorescence kinetics and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay showed that ALA PLGA NPs were more effective than free ALA of the same concentration.Conclusion: PLGA NPs provide a promising ALA delivery strategy for topical ALA-photodynamic therapy of skin squamous cell carcinoma.Keywords: 5-Aminolevulinic acid (ALA), nanoparticles, poly(lactic-co-glycolic acid) (PLGA), skin squamous cell carcinoma, photodynamic therapy (PDT)
format	article
author	Shi L Wang XL Zhao F Luan HS Tu QF Huang Z Wang H Wang HW
author_facet	Shi L Wang XL Zhao F Luan HS Tu QF Huang Z Wang H Wang HW
author_sort	Shi L
title	In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
title_short	In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
title_full	In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
title_fullStr	In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
title_full_unstemmed	In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
title_sort	in vitro evaluation of 5-aminolevulinic acid (ala) loaded plga nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/1a854943b04a480e998532c164b44b09
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In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles

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