Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques

Abstract An effective vaccine to prevent HIV transmission has not yet been achieved. Modulation of the microbiome via probiotic therapy has been suggested to result in enhanced mucosal immunity. Here, we evaluated whether probiotic therapy could improve the immunogenicity and protective efficacy of...

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Autores principales:	Nichole R. Klatt, Courtney Broedlow, Jessica M. Osborn, Andrew T. Gustin, Sandra Dross, Megan A. O’Connor, Ernesto Coronado, Philip Barnette, Tiffany Hensley-McBain, Alexander S. Zevin, Roshell Muir, Alexander Roederer, Solomon Wangari, Naoto Iwayama, Chul Y. Ahrens, Jeremy Smedley, Cassandra Moats, Rebecca M. Lynch, Elias K. Haddad, Nancy L. Haigwood, Deborah H. Fuller, Jennifer A. Manuzak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/1a878c0b5f054d51810e80949b2527b7
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spelling	oai:doaj.org-article:1a878c0b5f054d51810e80949b2527b72021-12-02T15:52:58ZEffects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques10.1038/s41541-021-00298-42059-0105https://doaj.org/article/1a878c0b5f054d51810e80949b2527b72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00298-4https://doaj.org/toc/2059-0105Abstract An effective vaccine to prevent HIV transmission has not yet been achieved. Modulation of the microbiome via probiotic therapy has been suggested to result in enhanced mucosal immunity. Here, we evaluated whether probiotic therapy could improve the immunogenicity and protective efficacy of SIV/HIV vaccination. Rhesus macaques were co-immunized with an SIV/HIV DNA vaccine via particle-mediated epidermal delivery and an HIV protein vaccine administered intramuscularly with Adjuplex™ adjuvant, while receiving daily oral Visbiome® probiotics. Probiotic therapy alone led to reduced frequencies of colonic CCR5+ and CCR6+ CD4+ T cells. Probiotics with SIV/HIV vaccination led to similar reductions in colonic CCR5+ CD4+ T cell frequencies. SIV/HIV-specific T cell and antibody responses were readily detected in the periphery of vaccinated animals but were not enhanced with probiotic treatment. Combination probiotics and vaccination did not impact rectal SIV/HIV target populations or reduce the rate of heterologous SHIV acquisition during the intrarectal challenge. Finally, post-infection viral kinetics were similar between all groups. Thus, although probiotics were well-tolerated when administered with SIV/HIV vaccination, vaccine-specific responses were not significantly enhanced. Additional work will be necessary to develop more effective strategies of microbiome modulation in order to enhance mucosal vaccine immunogenicity and improve protective immune responses.Nichole R. KlattCourtney BroedlowJessica M. OsbornAndrew T. GustinSandra DrossMegan A. O’ConnorErnesto CoronadoPhilip BarnetteTiffany Hensley-McBainAlexander S. ZevinRoshell MuirAlexander RoedererSolomon WangariNaoto IwayamaChul Y. AhrensJeremy SmedleyCassandra MoatsRebecca M. LynchElias K. HaddadNancy L. HaigwoodDeborah H. FullerJennifer A. ManuzakNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Nichole R. Klatt Courtney Broedlow Jessica M. Osborn Andrew T. Gustin Sandra Dross Megan A. O’Connor Ernesto Coronado Philip Barnette Tiffany Hensley-McBain Alexander S. Zevin Roshell Muir Alexander Roederer Solomon Wangari Naoto Iwayama Chul Y. Ahrens Jeremy Smedley Cassandra Moats Rebecca M. Lynch Elias K. Haddad Nancy L. Haigwood Deborah H. Fuller Jennifer A. Manuzak Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
description	Abstract An effective vaccine to prevent HIV transmission has not yet been achieved. Modulation of the microbiome via probiotic therapy has been suggested to result in enhanced mucosal immunity. Here, we evaluated whether probiotic therapy could improve the immunogenicity and protective efficacy of SIV/HIV vaccination. Rhesus macaques were co-immunized with an SIV/HIV DNA vaccine via particle-mediated epidermal delivery and an HIV protein vaccine administered intramuscularly with Adjuplex™ adjuvant, while receiving daily oral Visbiome® probiotics. Probiotic therapy alone led to reduced frequencies of colonic CCR5+ and CCR6+ CD4+ T cells. Probiotics with SIV/HIV vaccination led to similar reductions in colonic CCR5+ CD4+ T cell frequencies. SIV/HIV-specific T cell and antibody responses were readily detected in the periphery of vaccinated animals but were not enhanced with probiotic treatment. Combination probiotics and vaccination did not impact rectal SIV/HIV target populations or reduce the rate of heterologous SHIV acquisition during the intrarectal challenge. Finally, post-infection viral kinetics were similar between all groups. Thus, although probiotics were well-tolerated when administered with SIV/HIV vaccination, vaccine-specific responses were not significantly enhanced. Additional work will be necessary to develop more effective strategies of microbiome modulation in order to enhance mucosal vaccine immunogenicity and improve protective immune responses.
format	article
author	Nichole R. Klatt Courtney Broedlow Jessica M. Osborn Andrew T. Gustin Sandra Dross Megan A. O’Connor Ernesto Coronado Philip Barnette Tiffany Hensley-McBain Alexander S. Zevin Roshell Muir Alexander Roederer Solomon Wangari Naoto Iwayama Chul Y. Ahrens Jeremy Smedley Cassandra Moats Rebecca M. Lynch Elias K. Haddad Nancy L. Haigwood Deborah H. Fuller Jennifer A. Manuzak
author_facet	Nichole R. Klatt Courtney Broedlow Jessica M. Osborn Andrew T. Gustin Sandra Dross Megan A. O’Connor Ernesto Coronado Philip Barnette Tiffany Hensley-McBain Alexander S. Zevin Roshell Muir Alexander Roederer Solomon Wangari Naoto Iwayama Chul Y. Ahrens Jeremy Smedley Cassandra Moats Rebecca M. Lynch Elias K. Haddad Nancy L. Haigwood Deborah H. Fuller Jennifer A. Manuzak
author_sort	Nichole R. Klatt
title	Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
title_short	Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
title_full	Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
title_fullStr	Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
title_full_unstemmed	Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
title_sort	effects of persistent modulation of intestinal microbiota on siv/hiv vaccination in rhesus macaques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1a878c0b5f054d51810e80949b2527b7
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Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques

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