<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features

Lactic acid bacteria (LAB) share several beneficial effects on human organisms, such as bioactive metabolites’ release, pathogens’ competition and immune stimulation. This study aimed at determining the probiotic potential of autochthonous lactic acid bacteria isolated from carrots. In particular, t...

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Autores principales:	Emily Schifano, Alberta Tomassini, Adele Preziosi, Jorge Montes, Walter Aureli, Patrizia Mancini, Alfredo Miccheli, Daniela Uccelletti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	probiotic carrots <i>Caenorhabditis</i> <i>elegans</i> pathogen resistance Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1a88840b9227472fad6e4a123050011a
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spelling	oai:doaj.org-article:1a88840b9227472fad6e4a123050011a2021-11-25T18:24:53Z<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features10.3390/microorganisms91122902076-2607https://doaj.org/article/1a88840b9227472fad6e4a123050011a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2290https://doaj.org/toc/2076-2607Lactic acid bacteria (LAB) share several beneficial effects on human organisms, such as bioactive metabolites’ release, pathogens’ competition and immune stimulation. This study aimed at determining the probiotic potential of autochthonous lactic acid bacteria isolated from carrots. In particular, the work reported the characterization at the species level of four LAB strains deriving from carrots harvested in Fucino highland, Abruzzo (Italy). Ribosomal 16S DNA analysis allowed identification of three strains belonging to <i>Leuconostoc</i> <i>mesenteroides</i> and a <i>Weissella</i> <i>soli</i> strain. In vitro and in vivo assays were performed to investigate the probiotic potential of the different isolates. Among them, <i>L.</i> <i>mesenteroides</i> C2 and <i>L.</i> <i>mesenteroides</i> C7 showed high survival percentages under in vitro simulated gastro-intestinal conditions, antibiotic susceptibly and the ability to inhibit in vitro growth against <i>Salmonella</i> <i>enterica</i> serovar <i>Typhimurium</i>, <i>Listeria</i> <i>monocytogenes</i>, <i>Pseudomonas</i> <i>aeruginosa</i> and <i>Staphylococcus</i> <i>aureus</i> pathogens. In parallel, the simple model <i>Caenorhabditis</i> <i>elegans</i> was used for in vivo screenings. <i>L.</i> <i>mesenteroides</i> C2 and <i>L.</i> <i>mesenteroides</i> C7 strains significantly induced pro-longevity effects, protection from pathogens’ infection and innate immunity stimulation. Overall, these results showed that some autochthonous LAB from vegetables such as carrots have functional features to be considered as novel probiotic candidates.Emily SchifanoAlberta TomassiniAdele PreziosiJorge MontesWalter AureliPatrizia ManciniAlfredo MiccheliDaniela UccellettiMDPI AGarticleprobioticcarrots<i>Caenorhabditis</i> <i>elegans</i>pathogen resistanceBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2290, p 2290 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic carrots <i>Caenorhabditis</i> <i>elegans</i> pathogen resistance Biology (General) QH301-705.5
spellingShingle	probiotic carrots <i>Caenorhabditis</i> <i>elegans</i> pathogen resistance Biology (General) QH301-705.5 Emily Schifano Alberta Tomassini Adele Preziosi Jorge Montes Walter Aureli Patrizia Mancini Alfredo Miccheli Daniela Uccelletti <i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
description	Lactic acid bacteria (LAB) share several beneficial effects on human organisms, such as bioactive metabolites’ release, pathogens’ competition and immune stimulation. This study aimed at determining the probiotic potential of autochthonous lactic acid bacteria isolated from carrots. In particular, the work reported the characterization at the species level of four LAB strains deriving from carrots harvested in Fucino highland, Abruzzo (Italy). Ribosomal 16S DNA analysis allowed identification of three strains belonging to <i>Leuconostoc</i> <i>mesenteroides</i> and a <i>Weissella</i> <i>soli</i> strain. In vitro and in vivo assays were performed to investigate the probiotic potential of the different isolates. Among them, <i>L.</i> <i>mesenteroides</i> C2 and <i>L.</i> <i>mesenteroides</i> C7 showed high survival percentages under in vitro simulated gastro-intestinal conditions, antibiotic susceptibly and the ability to inhibit in vitro growth against <i>Salmonella</i> <i>enterica</i> serovar <i>Typhimurium</i>, <i>Listeria</i> <i>monocytogenes</i>, <i>Pseudomonas</i> <i>aeruginosa</i> and <i>Staphylococcus</i> <i>aureus</i> pathogens. In parallel, the simple model <i>Caenorhabditis</i> <i>elegans</i> was used for in vivo screenings. <i>L.</i> <i>mesenteroides</i> C2 and <i>L.</i> <i>mesenteroides</i> C7 strains significantly induced pro-longevity effects, protection from pathogens’ infection and innate immunity stimulation. Overall, these results showed that some autochthonous LAB from vegetables such as carrots have functional features to be considered as novel probiotic candidates.
format	article
author	Emily Schifano Alberta Tomassini Adele Preziosi Jorge Montes Walter Aureli Patrizia Mancini Alfredo Miccheli Daniela Uccelletti
author_facet	Emily Schifano Alberta Tomassini Adele Preziosi Jorge Montes Walter Aureli Patrizia Mancini Alfredo Miccheli Daniela Uccelletti
author_sort	Emily Schifano
title	<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
title_short	<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
title_full	<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
title_fullStr	<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
title_full_unstemmed	<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features
title_sort	<i>leuconostoc</i> <i>mesenteroides</i> strains isolated from carrots show probiotic features
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a88840b9227472fad6e4a123050011a
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<i>Leuconostoc</i> <i>mesenteroides</i> Strains Isolated from Carrots Show Probiotic Features

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