Phage Cocktail Development against Aeromonas salmonicida subsp. salmonicida Strains Is Compromised by a Prophage

Aquaculture is a rapidly growing food production sector. Fish farmers are experiencing increasing problems with antibiotic resistance when fighting against pathogenic bacteria such as Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis....

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Autores principales:	Nava Hosseini, Valérie E. Paquet, Mahdi Chehreghani, Sylvain Moineau, Steve J. Charette
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> furunculosis phage therapy phage cocktail <i>AsaGEI</i> Prophage 3 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1a9114411ee441fab3da9ec478832896
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spelling	oai:doaj.org-article:1a9114411ee441fab3da9ec4788328962021-11-25T19:13:41ZPhage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage10.3390/v131122411999-4915https://doaj.org/article/1a9114411ee441fab3da9ec4788328962021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2241https://doaj.org/toc/1999-4915Aquaculture is a rapidly growing food production sector. Fish farmers are experiencing increasing problems with antibiotic resistance when fighting against pathogenic bacteria such as <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i>, the causative agent of furunculosis. Phage therapy may provide an alternative, but effective use must be determined. Here, we studied the inhibition of <i>A. salmonicida</i> subsp. <i>salmonicida</i> strains by five phages (HER98 [44RR2.8t.2], HER110 [65.2], SW69-9, L9-6 and Riv-10) used individually or as combinations of two to five phages. A particular combination of four phages (HER98 [44RR2.8t.2], SW69-9, Riv-10, and HER110 [65.2]) was found to be the most effective when used at an initial multiplicity of infection (MOI) of 1 against the <i>A. salmonicida</i> subsp. <i>salmonicida</i> strain 01-B526. The same phage cocktail is effective against other strains except those bearing a prophage (named Prophage 3), which is present in 2/3 of the strains from the province of Quebec. To confirm the impact of this prophage, we tested the effectiveness of the same cocktail on strains that were either cured or lysogenized with Prophage 3. While the parental strains were sensitive to the phage cocktail, the lysogenized ones were much less sensitive. These data indicate that the prophage content of <i>A. salmonicida</i> subsp. <i>salmonicida</i> can affect the efficacy of a cocktail of virulent phages for phage therapy purposes.Nava HosseiniValérie E. PaquetMahdi ChehreghaniSylvain MoineauSteve J. CharetteMDPI AGarticle<i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i>furunculosisphage therapyphage cocktail<i>AsaGEI</i>Prophage 3MicrobiologyQR1-502ENViruses, Vol 13, Iss 2241, p 2241 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> furunculosis phage therapy phage cocktail <i>AsaGEI</i> Prophage 3 Microbiology QR1-502
spellingShingle	<i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> furunculosis phage therapy phage cocktail <i>AsaGEI</i> Prophage 3 Microbiology QR1-502 Nava Hosseini Valérie E. Paquet Mahdi Chehreghani Sylvain Moineau Steve J. Charette Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
description	Aquaculture is a rapidly growing food production sector. Fish farmers are experiencing increasing problems with antibiotic resistance when fighting against pathogenic bacteria such as <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i>, the causative agent of furunculosis. Phage therapy may provide an alternative, but effective use must be determined. Here, we studied the inhibition of <i>A. salmonicida</i> subsp. <i>salmonicida</i> strains by five phages (HER98 [44RR2.8t.2], HER110 [65.2], SW69-9, L9-6 and Riv-10) used individually or as combinations of two to five phages. A particular combination of four phages (HER98 [44RR2.8t.2], SW69-9, Riv-10, and HER110 [65.2]) was found to be the most effective when used at an initial multiplicity of infection (MOI) of 1 against the <i>A. salmonicida</i> subsp. <i>salmonicida</i> strain 01-B526. The same phage cocktail is effective against other strains except those bearing a prophage (named Prophage 3), which is present in 2/3 of the strains from the province of Quebec. To confirm the impact of this prophage, we tested the effectiveness of the same cocktail on strains that were either cured or lysogenized with Prophage 3. While the parental strains were sensitive to the phage cocktail, the lysogenized ones were much less sensitive. These data indicate that the prophage content of <i>A. salmonicida</i> subsp. <i>salmonicida</i> can affect the efficacy of a cocktail of virulent phages for phage therapy purposes.
format	article
author	Nava Hosseini Valérie E. Paquet Mahdi Chehreghani Sylvain Moineau Steve J. Charette
author_facet	Nava Hosseini Valérie E. Paquet Mahdi Chehreghani Sylvain Moineau Steve J. Charette
author_sort	Nava Hosseini
title	Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
title_short	Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
title_full	Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
title_fullStr	Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
title_full_unstemmed	Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage
title_sort	phage cocktail development against <i>aeromonas salmonicida</i> subsp. <i>salmonicida</i> strains is compromised by a prophage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a9114411ee441fab3da9ec478832896
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Phage Cocktail Development against <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains Is Compromised by a Prophage

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