Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide
Abstract Following bacterial infection, macrophages produce pro-inflammatory cytokines in response to bacterial cell components, including lipopolysaccharide (LPS) and lipopeptide, and simultaneously phagocytize and digest the invading bacteria. To study the effects of phagocytosis on pro-inflammato...
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2021
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oai:doaj.org-article:1a95e0e879af48899b7239805f284e762021-12-02T13:24:14ZPhagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide10.1038/s41598-021-86202-52045-2322https://doaj.org/article/1a95e0e879af48899b7239805f284e762021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86202-5https://doaj.org/toc/2045-2322Abstract Following bacterial infection, macrophages produce pro-inflammatory cytokines in response to bacterial cell components, including lipopolysaccharide (LPS) and lipopeptide, and simultaneously phagocytize and digest the invading bacteria. To study the effects of phagocytosis on pro-inflammatory responses, we determined if phagocytosis of polystyrene latex beads with ~ 1 µm diameter increases pro-inflammatory cytokine expression by human macrophage-like U937 and THP-1 cells stimulated with LPS. Treating macrophage-like cells with beads coated with IgG to facilitate Fcγ receptor-mediated phagocytosis increased LPS-induced expression of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. Treatment with beads coated with poly-l-lysine to facilitate Fcγ receptor–independent phagocytosis also increased LPS-induced cytokine expression. Our results indicate that LPS-induced pro-inflammatory responses are enhanced by bead phagocytosis regardless of the uptake mechanism. Additionally, phagocytosis enhanced LPS-induced NF-κB activation, suggesting that Toll-like receptor (TLR) 4 signaling is enhanced by phagocytosis. Furthermore, bead phagocytosis enhanced pro-inflammatory responses in U937 cells stimulated with lipopeptide, a ligand for the TLR2/TLR6 heterodimeric receptor. In conclusion, microparticle phagocytosis by macrophage-like U937 and THP-1 cells enhances the innate immune response induced by bacterial components.Takayuki UenoYumi YamamotoKiyoshi KawasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
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Medicine R Science Q Takayuki Ueno Yumi Yamamoto Kiyoshi Kawasaki Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
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Abstract Following bacterial infection, macrophages produce pro-inflammatory cytokines in response to bacterial cell components, including lipopolysaccharide (LPS) and lipopeptide, and simultaneously phagocytize and digest the invading bacteria. To study the effects of phagocytosis on pro-inflammatory responses, we determined if phagocytosis of polystyrene latex beads with ~ 1 µm diameter increases pro-inflammatory cytokine expression by human macrophage-like U937 and THP-1 cells stimulated with LPS. Treating macrophage-like cells with beads coated with IgG to facilitate Fcγ receptor-mediated phagocytosis increased LPS-induced expression of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. Treatment with beads coated with poly-l-lysine to facilitate Fcγ receptor–independent phagocytosis also increased LPS-induced cytokine expression. Our results indicate that LPS-induced pro-inflammatory responses are enhanced by bead phagocytosis regardless of the uptake mechanism. Additionally, phagocytosis enhanced LPS-induced NF-κB activation, suggesting that Toll-like receptor (TLR) 4 signaling is enhanced by phagocytosis. Furthermore, bead phagocytosis enhanced pro-inflammatory responses in U937 cells stimulated with lipopeptide, a ligand for the TLR2/TLR6 heterodimeric receptor. In conclusion, microparticle phagocytosis by macrophage-like U937 and THP-1 cells enhances the innate immune response induced by bacterial components. |
format |
article |
author |
Takayuki Ueno Yumi Yamamoto Kiyoshi Kawasaki |
author_facet |
Takayuki Ueno Yumi Yamamoto Kiyoshi Kawasaki |
author_sort |
Takayuki Ueno |
title |
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
title_short |
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
title_full |
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
title_fullStr |
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
title_full_unstemmed |
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide |
title_sort |
phagocytosis of microparticles increases responsiveness of macrophage-like cell lines u937 and thp-1 to bacterial lipopolysaccharide and lipopeptide |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/1a95e0e879af48899b7239805f284e76 |
work_keys_str_mv |
AT takayukiueno phagocytosisofmicroparticlesincreasesresponsivenessofmacrophagelikecelllinesu937andthp1tobacteriallipopolysaccharideandlipopeptide AT yumiyamamoto phagocytosisofmicroparticlesincreasesresponsivenessofmacrophagelikecelllinesu937andthp1tobacteriallipopolysaccharideandlipopeptide AT kiyoshikawasaki phagocytosisofmicroparticlesincreasesresponsivenessofmacrophagelikecelllinesu937andthp1tobacteriallipopolysaccharideandlipopeptide |
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