Glycans as Potential Diagnostic Markers of Traumatic Brain Injury

The diagnosis of mild traumatic brain injury (TBI) is challenging in the acute setting because the symptoms are nonspecific and often transient, or they develop with a delay. In these cases, the criteria for acute head imaging are frequently not fulfilled. This may lead to missed diagnoses in emerge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mårten Kvist, Lasse Välimaa, Adrian Harel, Jussi P. Posti, Melissa Rahi, Ilkka Saarenpää, Mikko Visuri, Anna Östberg, Jaakko Rinne
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	TBI lectin glycan biomarker concussion traumatic brain injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/1a9c72401e8f45bea7417fd8f516806a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1a9c72401e8f45bea7417fd8f516806a
record_format	dspace
spelling	oai:doaj.org-article:1a9c72401e8f45bea7417fd8f516806a2021-11-25T16:58:11ZGlycans as Potential Diagnostic Markers of Traumatic Brain Injury10.3390/brainsci111114802076-3425https://doaj.org/article/1a9c72401e8f45bea7417fd8f516806a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3425/11/11/1480https://doaj.org/toc/2076-3425The diagnosis of mild traumatic brain injury (TBI) is challenging in the acute setting because the symptoms are nonspecific and often transient, or they develop with a delay. In these cases, the criteria for acute head imaging are frequently not fulfilled. This may lead to missed diagnoses in emergency care. There is a need for developing a rapid diagnostic test to verify the presence of TBI using body fluids. Blood, urine, and saliva samples from 11 adult patients (mean age 64 years, SD 24 years) with acute and clinically diagnosed TBI, and 12 healthy volunteers were collected at Turku University Hospital during a period of 5 months. The injuries necessitated hospitalization for at least one day. The TBIs were classified mild in nine cases and severe in two cases. The mean period between the trauma and the time for obtaining the samples was 27 h, SD 11 h. The samples were analyzed in an ISO-certified laboratory for the number of lectin-bound glycan molecules indicating destruction of nerve tissue. The screening was performed on several possible glycans for binding, and the measurement by degree of fluorescence. In the analysis, the group of patients with TBI was compared with healthy volunteers. The results showed a significant decrease (<i>p</i> < 0.05, Wilcoxon rank–sum two-sided test) in the level of two glycans in <i>plasma</i>, but no significant increase for any glycan; in <i>saliva</i>, one glycan showed a significant increase in the TBI group; in <i>urine</i>, three glycans were significantly different between the groups (one showed an increase, whereas two showed a decrease). The results support the idea of conducting more research on how diagnostic glycans could be detected in body fluids after TBI. As a proof-of-concept, significant changes in the concentration of five glycans were found in plasma, saliva, and urine between TBI patients and healthy controls. This may enable the development of a rapid body fluid-based point-of-care test to identify patients with TBI after a head injury.Mårten KvistLasse VälimaaAdrian HarelJussi P. PostiMelissa RahiIlkka SaarenpääMikko VisuriAnna ÖstbergJaakko RinneMDPI AGarticleTBIlectinglycanbiomarkerconcussiontraumatic brain injuryNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBrain Sciences, Vol 11, Iss 1480, p 1480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TBI lectin glycan biomarker concussion traumatic brain injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	TBI lectin glycan biomarker concussion traumatic brain injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Mårten Kvist Lasse Välimaa Adrian Harel Jussi P. Posti Melissa Rahi Ilkka Saarenpää Mikko Visuri Anna Östberg Jaakko Rinne Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
description	The diagnosis of mild traumatic brain injury (TBI) is challenging in the acute setting because the symptoms are nonspecific and often transient, or they develop with a delay. In these cases, the criteria for acute head imaging are frequently not fulfilled. This may lead to missed diagnoses in emergency care. There is a need for developing a rapid diagnostic test to verify the presence of TBI using body fluids. Blood, urine, and saliva samples from 11 adult patients (mean age 64 years, SD 24 years) with acute and clinically diagnosed TBI, and 12 healthy volunteers were collected at Turku University Hospital during a period of 5 months. The injuries necessitated hospitalization for at least one day. The TBIs were classified mild in nine cases and severe in two cases. The mean period between the trauma and the time for obtaining the samples was 27 h, SD 11 h. The samples were analyzed in an ISO-certified laboratory for the number of lectin-bound glycan molecules indicating destruction of nerve tissue. The screening was performed on several possible glycans for binding, and the measurement by degree of fluorescence. In the analysis, the group of patients with TBI was compared with healthy volunteers. The results showed a significant decrease (<i>p</i> < 0.05, Wilcoxon rank–sum two-sided test) in the level of two glycans in <i>plasma</i>, but no significant increase for any glycan; in <i>saliva</i>, one glycan showed a significant increase in the TBI group; in <i>urine</i>, three glycans were significantly different between the groups (one showed an increase, whereas two showed a decrease). The results support the idea of conducting more research on how diagnostic glycans could be detected in body fluids after TBI. As a proof-of-concept, significant changes in the concentration of five glycans were found in plasma, saliva, and urine between TBI patients and healthy controls. This may enable the development of a rapid body fluid-based point-of-care test to identify patients with TBI after a head injury.
format	article
author	Mårten Kvist Lasse Välimaa Adrian Harel Jussi P. Posti Melissa Rahi Ilkka Saarenpää Mikko Visuri Anna Östberg Jaakko Rinne
author_facet	Mårten Kvist Lasse Välimaa Adrian Harel Jussi P. Posti Melissa Rahi Ilkka Saarenpää Mikko Visuri Anna Östberg Jaakko Rinne
author_sort	Mårten Kvist
title	Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
title_short	Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
title_full	Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
title_fullStr	Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
title_full_unstemmed	Glycans as Potential Diagnostic Markers of Traumatic Brain Injury
title_sort	glycans as potential diagnostic markers of traumatic brain injury
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a9c72401e8f45bea7417fd8f516806a
work_keys_str_mv	AT martenkvist glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT lassevalimaa glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT adrianharel glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT jussipposti glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT melissarahi glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT ilkkasaarenpaa glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT mikkovisuri glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT annaostberg glycansaspotentialdiagnosticmarkersoftraumaticbraininjury AT jaakkorinne glycansaspotentialdiagnosticmarkersoftraumaticbraininjury
_version_	1718412821944860672

Glycans as Potential Diagnostic Markers of Traumatic Brain Injury

Ejemplares similares