A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model

Abstract Allelic variants of the pan-haematopoietic cell marker CD45, identified as CD45.1 and CD45.2, have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants. Despite the frequent use of this model for studying the impact of genetic mo...

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Autores principales:	Salema Jafri, Stephen D. Moore, Nicholas W. Morrell, Mark L. Ormiston
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a9f8a796a894ab1b5b27e661ebbdf59
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spelling	oai:doaj.org-article:1a9f8a796a894ab1b5b27e661ebbdf592021-12-02T15:05:22ZA sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model10.1038/s41598-017-03784-92045-2322https://doaj.org/article/1a9f8a796a894ab1b5b27e661ebbdf592017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03784-9https://doaj.org/toc/2045-2322Abstract Allelic variants of the pan-haematopoietic cell marker CD45, identified as CD45.1 and CD45.2, have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants. Despite the frequent use of this model for studying the impact of genetic modifications on relative differentiation potential, it is now evident that a bias exists in CD45.1 versus CD45.2 cell reconstitution. While this bias has been demonstrated by reduced reconstitution potential in B cells of CD45.1 origin, differences in the development of other lymphocytes, as well as the impact of sex on this bias, remain uncertain. We performed bone marrow transplants with wild-type CD45.1 and CD45.2 donor cells, and characterised haematopoietic cell reconstitution in dual-expressing CD45.1/2 host mice. We report an increase in CD45.2 reconstitution in the bone marrow that persists in the spleen, thymus and blood. Through the use of CD45.1/2 hosts, we demonstrate the intrinsic bias towards CD45.2 reconstitution is independent of an immunogenic response to the CD45.1 epitope. Furthermore, we identify a sex-specific difference in reconstitution efficiencies, with female mice exhibiting a greater bias towards CD45.2 reconstitution than males. This work sheds new light on the limitations of the CD45.1/CD45.2 congenic system for tracking lymphocyte development.Salema JafriStephen D. MooreNicholas W. MorrellMark L. OrmistonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Salema Jafri Stephen D. Moore Nicholas W. Morrell Mark L. Ormiston A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
description	Abstract Allelic variants of the pan-haematopoietic cell marker CD45, identified as CD45.1 and CD45.2, have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants. Despite the frequent use of this model for studying the impact of genetic modifications on relative differentiation potential, it is now evident that a bias exists in CD45.1 versus CD45.2 cell reconstitution. While this bias has been demonstrated by reduced reconstitution potential in B cells of CD45.1 origin, differences in the development of other lymphocytes, as well as the impact of sex on this bias, remain uncertain. We performed bone marrow transplants with wild-type CD45.1 and CD45.2 donor cells, and characterised haematopoietic cell reconstitution in dual-expressing CD45.1/2 host mice. We report an increase in CD45.2 reconstitution in the bone marrow that persists in the spleen, thymus and blood. Through the use of CD45.1/2 hosts, we demonstrate the intrinsic bias towards CD45.2 reconstitution is independent of an immunogenic response to the CD45.1 epitope. Furthermore, we identify a sex-specific difference in reconstitution efficiencies, with female mice exhibiting a greater bias towards CD45.2 reconstitution than males. This work sheds new light on the limitations of the CD45.1/CD45.2 congenic system for tracking lymphocyte development.
format	article
author	Salema Jafri Stephen D. Moore Nicholas W. Morrell Mark L. Ormiston
author_facet	Salema Jafri Stephen D. Moore Nicholas W. Morrell Mark L. Ormiston
author_sort	Salema Jafri
title	A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
title_short	A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
title_full	A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
title_fullStr	A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
title_full_unstemmed	A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model
title_sort	sex-specific reconstitution bias in the competitive cd45.1/cd45.2 congenic bone marrow transplant model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1a9f8a796a894ab1b5b27e661ebbdf59
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_version_	1718388816050388992

A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model

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