Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo

Abstract Trachoma, caused by repeated ocular infection with Chlamydia trachomatis (Ct), is targeted for elimination as a public health problem. Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings wi...

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Autores principales:	Sarah Gwyn, Marcel S. Awoussi, Ana Bakhtiari, Rachel N. Bronzan, Kathryn Crowley, Emma M. Harding-Esch, Yao Kassankogno, Janvier N. Kilangalanga, Felix Makangila, Sylvain Mupoyi, Jeremiah Ngondi, Bonaventure Ngoyi, Stephanie Palmer, Jessica M. Randall, Anders Seim, Anthony W. Solomon, Raymond Stewart, Kwamy Togbey, Pitchouna A. Uvon, Diana L. Martin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1aa618acbb8a4fdd9322558291fc8bf3
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spelling	oai:doaj.org-article:1aa618acbb8a4fdd9322558291fc8bf32021-12-02T18:17:41ZComparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo10.1038/s41598-021-86639-82045-2322https://doaj.org/article/1aa618acbb8a4fdd9322558291fc8bf32021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86639-8https://doaj.org/toc/2045-2322Abstract Trachoma, caused by repeated ocular infection with Chlamydia trachomatis (Ct), is targeted for elimination as a public health problem. Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings with different prevalence of the indicator trachomatous inflammation—follicular (TF). Dried blood spots were collected during trachoma mapping in two districts each of Togo and Democratic Republic of the Congo. Anti-Ct antibodies were detected by multiplex bead assay (MBA) and three different lateral flow assays (LFA) and seroprevalence and seroconversion rate (SCR) were determined. By most tests, the district with > 5% TF (the elimination threshold) had five–sixfold higher seroprevalence and tenfold higher SCR than districts with < 5% TF. The agreement between LFA and MBA was improved using a black latex developing reagent. These data show optimization of antibody tests against Ct to better differentiate districts above or below trachoma elimination thresholds.Sarah GwynMarcel S. AwoussiAna BakhtiariRachel N. BronzanKathryn CrowleyEmma M. Harding-EschYao KassankognoJanvier N. KilangalangaFelix MakangilaSylvain MupoyiJeremiah NgondiBonaventure NgoyiStephanie PalmerJessica M. RandallAnders SeimAnthony W. SolomonRaymond StewartKwamy TogbeyPitchouna A. UvonDiana L. MartinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah Gwyn Marcel S. Awoussi Ana Bakhtiari Rachel N. Bronzan Kathryn Crowley Emma M. Harding-Esch Yao Kassankogno Janvier N. Kilangalanga Felix Makangila Sylvain Mupoyi Jeremiah Ngondi Bonaventure Ngoyi Stephanie Palmer Jessica M. Randall Anders Seim Anthony W. Solomon Raymond Stewart Kwamy Togbey Pitchouna A. Uvon Diana L. Martin Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
description	Abstract Trachoma, caused by repeated ocular infection with Chlamydia trachomatis (Ct), is targeted for elimination as a public health problem. Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings with different prevalence of the indicator trachomatous inflammation—follicular (TF). Dried blood spots were collected during trachoma mapping in two districts each of Togo and Democratic Republic of the Congo. Anti-Ct antibodies were detected by multiplex bead assay (MBA) and three different lateral flow assays (LFA) and seroprevalence and seroconversion rate (SCR) were determined. By most tests, the district with > 5% TF (the elimination threshold) had five–sixfold higher seroprevalence and tenfold higher SCR than districts with < 5% TF. The agreement between LFA and MBA was improved using a black latex developing reagent. These data show optimization of antibody tests against Ct to better differentiate districts above or below trachoma elimination thresholds.
format	article
author	Sarah Gwyn Marcel S. Awoussi Ana Bakhtiari Rachel N. Bronzan Kathryn Crowley Emma M. Harding-Esch Yao Kassankogno Janvier N. Kilangalanga Felix Makangila Sylvain Mupoyi Jeremiah Ngondi Bonaventure Ngoyi Stephanie Palmer Jessica M. Randall Anders Seim Anthony W. Solomon Raymond Stewart Kwamy Togbey Pitchouna A. Uvon Diana L. Martin
author_facet	Sarah Gwyn Marcel S. Awoussi Ana Bakhtiari Rachel N. Bronzan Kathryn Crowley Emma M. Harding-Esch Yao Kassankogno Janvier N. Kilangalanga Felix Makangila Sylvain Mupoyi Jeremiah Ngondi Bonaventure Ngoyi Stephanie Palmer Jessica M. Randall Anders Seim Anthony W. Solomon Raymond Stewart Kwamy Togbey Pitchouna A. Uvon Diana L. Martin
author_sort	Sarah Gwyn
title	Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
title_short	Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
title_full	Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
title_fullStr	Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
title_full_unstemmed	Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
title_sort	comparison of platforms for testing antibodies to chlamydia trachomatis antigens in the democratic republic of the congo and togo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1aa618acbb8a4fdd9322558291fc8bf3
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Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo

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