Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability

Tamiru N Alkie,1 Jondavid de Jong,1,2 Emily Moore,2 Stephanie J DeWitte-Orr1 1Department of Health Sciences, Wilfrid Laurier University, Waterloo, ON N2L 3C5, Canada; 2Glysantis Inc, Guelph, ON N1C 0A1, CanadaCorrespondence: Stephanie J DeWitte-OrrDepartment of Health Sciences, Wilfrid Laurier Unive...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alkie TN, de Jong J, Moore E, DeWitte-Orr SJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cytotoxicity bovine gpx phytoglycogen rainbow trout sodium selenite Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1aa8519d5147439584a9a7167f107271
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1aa8519d5147439584a9a7167f107271
record_format	dspace
spelling	oai:doaj.org-article:1aa8519d5147439584a9a7167f1072712021-12-02T13:57:15ZPhytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability1178-2013https://doaj.org/article/1aa8519d5147439584a9a7167f1072712020-12-01T00:00:00Zhttps://www.dovepress.com/phytoglycogen-nanoparticle-delivery-system-for-inorganic-selenium-redu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tamiru N Alkie,1 Jondavid de Jong,1,2 Emily Moore,2 Stephanie J DeWitte-Orr1 1Department of Health Sciences, Wilfrid Laurier University, Waterloo, ON N2L 3C5, Canada; 2Glysantis Inc, Guelph, ON N1C 0A1, CanadaCorrespondence: Stephanie J DeWitte-OrrDepartment of Health Sciences, Wilfrid Laurier University, Waterloo, ON N2L 3C5, CanadaTel +1 519 884 0710Email sdewitteorr@wlu.caPurpose: Selenium is an essential trace element that supports animal health through the antioxidant defense system by protecting cells from oxidative-related damage. Using inorganic selenium species, such as sodium selenite (Na Sel), as a food supplement is cost-effective; however, its limitation as a nutritional supplement is its cytotoxicity. One strategy to mitigate this problem is by delivering inorganic selenium using a nanoparticle delivery system (SeNP).Methods: Rainbow trout intestinal epithelial cells, bovine turbinate cells and bovine intestinal myofibroblasts were treated with soluble Na Sel or SeNPs. Two SeNP formulations were tested; SeNP-Ionic where inorganic selenium was ionically bound to cationic phytoglycogen (PhG) NPs, and SeNP-Covalent, where inorganic selenium was covalently bound to PhG NPs. Selenium-induced cytotoxicity along with selenium bioavailability were measured.Results: SeNPs (SeNP-Ionic or SeNP-Covalent) substantially reduced cytotoxicity in all cell types examined compared to similar doses of soluble inorganic selenium. The SeNP formulations did not affect selenium bioavailability, as selenium-induced glutathione peroxidase (GPx) activity and GPx1 transcript levels were similarly elevated whether cells were treated with soluble Na Sel or SeNPs. This was the case for all three cell types tested.Conclusion: Nanoparticle-assisted inorganic selenium delivery, which demonstrated equal bioavailability without causing deleterious cytotoxic side effects, has potential applications for safely supplementing animal diets with inorganic selenium at what are usually toxic doses.Keywords: cytotoxicity, bovine, GPx, phytoglycogen, rainbow trout, sodium seleniteAlkie TNde Jong JMoore EDeWitte-Orr SJDove Medical Pressarticlecytotoxicitybovinegpxphytoglycogenrainbow troutsodium seleniteMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 10469-10479 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytotoxicity bovine gpx phytoglycogen rainbow trout sodium selenite Medicine (General) R5-920
spellingShingle	cytotoxicity bovine gpx phytoglycogen rainbow trout sodium selenite Medicine (General) R5-920 Alkie TN de Jong J Moore E DeWitte-Orr SJ Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
description	Tamiru N Alkie,1 Jondavid de Jong,1,2 Emily Moore,2 Stephanie J DeWitte-Orr1 1Department of Health Sciences, Wilfrid Laurier University, Waterloo, ON N2L 3C5, Canada; 2Glysantis Inc, Guelph, ON N1C 0A1, CanadaCorrespondence: Stephanie J DeWitte-OrrDepartment of Health Sciences, Wilfrid Laurier University, Waterloo, ON N2L 3C5, CanadaTel +1 519 884 0710Email sdewitteorr@wlu.caPurpose: Selenium is an essential trace element that supports animal health through the antioxidant defense system by protecting cells from oxidative-related damage. Using inorganic selenium species, such as sodium selenite (Na Sel), as a food supplement is cost-effective; however, its limitation as a nutritional supplement is its cytotoxicity. One strategy to mitigate this problem is by delivering inorganic selenium using a nanoparticle delivery system (SeNP).Methods: Rainbow trout intestinal epithelial cells, bovine turbinate cells and bovine intestinal myofibroblasts were treated with soluble Na Sel or SeNPs. Two SeNP formulations were tested; SeNP-Ionic where inorganic selenium was ionically bound to cationic phytoglycogen (PhG) NPs, and SeNP-Covalent, where inorganic selenium was covalently bound to PhG NPs. Selenium-induced cytotoxicity along with selenium bioavailability were measured.Results: SeNPs (SeNP-Ionic or SeNP-Covalent) substantially reduced cytotoxicity in all cell types examined compared to similar doses of soluble inorganic selenium. The SeNP formulations did not affect selenium bioavailability, as selenium-induced glutathione peroxidase (GPx) activity and GPx1 transcript levels were similarly elevated whether cells were treated with soluble Na Sel or SeNPs. This was the case for all three cell types tested.Conclusion: Nanoparticle-assisted inorganic selenium delivery, which demonstrated equal bioavailability without causing deleterious cytotoxic side effects, has potential applications for safely supplementing animal diets with inorganic selenium at what are usually toxic doses.Keywords: cytotoxicity, bovine, GPx, phytoglycogen, rainbow trout, sodium selenite
format	article
author	Alkie TN de Jong J Moore E DeWitte-Orr SJ
author_facet	Alkie TN de Jong J Moore E DeWitte-Orr SJ
author_sort	Alkie TN
title	Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
title_short	Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
title_full	Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
title_fullStr	Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
title_full_unstemmed	Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability
title_sort	phytoglycogen nanoparticle delivery system for inorganic selenium reduces cytotoxicity without impairing selenium bioavailability
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/1aa8519d5147439584a9a7167f107271
work_keys_str_mv	AT alkietn phytoglycogennanoparticledeliverysystemforinorganicseleniumreducescytotoxicitywithoutimpairingseleniumbioavailability AT dejongj phytoglycogennanoparticledeliverysystemforinorganicseleniumreducescytotoxicitywithoutimpairingseleniumbioavailability AT mooree phytoglycogennanoparticledeliverysystemforinorganicseleniumreducescytotoxicitywithoutimpairingseleniumbioavailability AT dewitteorrsj phytoglycogennanoparticledeliverysystemforinorganicseleniumreducescytotoxicitywithoutimpairingseleniumbioavailability
_version_	1718392307346046976

Phytoglycogen Nanoparticle Delivery System for Inorganic Selenium Reduces Cytotoxicity without Impairing Selenium Bioavailability

Ejemplares similares