Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis

Background: Ionotropic glutamate NMDA receptors are multi-subunit proteins with few selective pharmacological ligands and are tentatively implicated in MS and other neurodegenerative disorders. The present study was aimed at evaluating the antioxidant properties of Berberis vulgaris extract-loaded m...

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Autores principales:	Fatemeh Noruzifard, Amaneh Javid, Seyed Mohsen Miresmaeili
Formato:	article
Lenguaje:	EN
Publicado:	Shahrekord University of Medical Sciences 2021
Materias:	nmda neuroprotection grin1 syrian mice berberis vulgaris Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1aa8bcd159b24e708a927f6a70d0c143
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spelling	oai:doaj.org-article:1aa8bcd159b24e708a927f6a70d0c1432021-11-13T05:12:32ZBerberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis2423-3579https://doaj.org/article/1aa8bcd159b24e708a927f6a70d0c1432021-10-01T00:00:00Zhttp://herbmed.skums.ac.ir/article_246499_2f4322b7774175188c5e7f8149b8331b.pdfhttps://doaj.org/toc/2423-3579Background: Ionotropic glutamate NMDA receptors are multi-subunit proteins with few selective pharmacological ligands and are tentatively implicated in MS and other neurodegenerative disorders. The present study was aimed at evaluating the antioxidant properties of Berberis vulgaris extract-loaded magnetite nanoparticles on the Grin1 gene expression in NMDA receptor in EAE Syrian mice. Methods: EAE mice models were generated through active immunization with MBP and PTx and kept for days 9-14 until EAE signs appeared followed by administration of barberry extract loaded magnetic nanoparticles. Results: Pure BE concentrations did not show recovery signs until days 7-9, but partial recovery in tail movement was seen on days 11 and 14, which was significant as compared to the control group in terms of improvement of the clinical scores. Meanwhile bare nanoparticles had neither disease recovery/progression properties nor EAE mice mortality as compared to controls, but 1 mg BE + Fe3O4 reduced EAE symptom severity and resulted in significant improvement of hind limb sensitivity to toe pinching and improved tail movements. Meanwhile 2 mg Be + Fe3O4 showed much better sensitivity to toe pinching and complete tail recovery. qRT-PCR analysis showed a significant decrease in relative Grin1 expression in female mice after treatment with 0.2 and 1 mg BE. However, a profound decrease in Grin1 expression was seen at 0.2, 1 and 2 mg BE + Fe3O4 treated groups in a dose-dependent manner. Conclusion: The results indicated that Fe3O4+ BE could alleviate the EAE severity and progressionFatemeh NoruzifardAmaneh JavidSeyed Mohsen MiresmaeiliShahrekord University of Medical Sciencesarticlenmdaneuroprotectiongrin1syrian miceberberis vulgarisMedicineRScienceQENAdvanced Herbal Medicine , Vol 6, Iss 2, Pp 49-74 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nmda neuroprotection grin1 syrian mice berberis vulgaris Medicine R Science Q
spellingShingle	nmda neuroprotection grin1 syrian mice berberis vulgaris Medicine R Science Q Fatemeh Noruzifard Amaneh Javid Seyed Mohsen Miresmaeili Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
description	Background: Ionotropic glutamate NMDA receptors are multi-subunit proteins with few selective pharmacological ligands and are tentatively implicated in MS and other neurodegenerative disorders. The present study was aimed at evaluating the antioxidant properties of Berberis vulgaris extract-loaded magnetite nanoparticles on the Grin1 gene expression in NMDA receptor in EAE Syrian mice. Methods: EAE mice models were generated through active immunization with MBP and PTx and kept for days 9-14 until EAE signs appeared followed by administration of barberry extract loaded magnetic nanoparticles. Results: Pure BE concentrations did not show recovery signs until days 7-9, but partial recovery in tail movement was seen on days 11 and 14, which was significant as compared to the control group in terms of improvement of the clinical scores. Meanwhile bare nanoparticles had neither disease recovery/progression properties nor EAE mice mortality as compared to controls, but 1 mg BE + Fe3O4 reduced EAE symptom severity and resulted in significant improvement of hind limb sensitivity to toe pinching and improved tail movements. Meanwhile 2 mg Be + Fe3O4 showed much better sensitivity to toe pinching and complete tail recovery. qRT-PCR analysis showed a significant decrease in relative Grin1 expression in female mice after treatment with 0.2 and 1 mg BE. However, a profound decrease in Grin1 expression was seen at 0.2, 1 and 2 mg BE + Fe3O4 treated groups in a dose-dependent manner. Conclusion: The results indicated that Fe3O4+ BE could alleviate the EAE severity and progression
format	article
author	Fatemeh Noruzifard Amaneh Javid Seyed Mohsen Miresmaeili
author_facet	Fatemeh Noruzifard Amaneh Javid Seyed Mohsen Miresmaeili
author_sort	Fatemeh Noruzifard
title	Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
title_short	Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
title_full	Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
title_fullStr	Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
title_full_unstemmed	Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis
title_sort	berberis vulgaris extract-based fe3o4 nanocomposites affect nmda1 function and physical activity: analysis of grin1 expression in syrian mice model of experimental autoimmune encephalomyelitis
publisher	Shahrekord University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/1aa8bcd159b24e708a927f6a70d0c143
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Berberis vulgaris extract-based Fe3O4 nanocomposites affect NMDA1 function and physical activity: Analysis of Grin1 expression in Syrian mice model of Experimental autoimmune encephalomyelitis

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