Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet

BACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract. OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2’-Deoxy Guanosine) and the number of foam cell....

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Autores principales:	Agnes Budiarto, Taufiqurrachman Nasihun, Atina Hussaana
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2018
Materias:	celery 8-ohdg foam cell Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1aa8bd6961e74bba87b358b662980255
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spelling	oai:doaj.org-article:1aa8bd6961e74bba87b358b6629802552021-11-25T13:15:53ZEffect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet2085-15452339-093X10.26532/sainsmed.v8i2.2421https://doaj.org/article/1aa8bd6961e74bba87b358b6629802552018-03-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/2421https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XBACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract. OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2’-Deoxy Guanosine) and the number of foam cell. METHODS: Experimental studies with post-test only control group design used Wistar rats, administered with adrenaline and high-fat diet for hyperlipidemic induction.Total of 35 rats were divided into 5 groups: negative controls (Neg-G); positive control treated with simvastatin (Nor-G); ES25-G,ES50-G, and ES75-G group were treated with 25 mg/200 g BW, 50 mg/200 g BW, and 75 mg/200 g BW celery extract, respectively. The treatment was given for 30 days. The 8-OHdG level was determined by ELISA, whereas the number of foam cells was determined by histopathologic preparations with HE staining. Data analysis was conducted by Kruskal-Wallis and Mann-Whitney. RESULTS: Mann-Whitney analysis showed levels of 8-OHdG in ES-25-G 12.13±0.21, ES-50-G 7.23±0.25, ES-75-G 4.41±0.45 significantly lower than the Nor-G 14.30±0.66, respectively p<0.001. The number of foam cells in the ES-25-G was 7.57±0.53, ES-50-G 6.57±0.79, ES-75-G 3.43±0.53,significantly lower than the Neg-G 13.57±1.27, respectively p<0.001. CONCLUSION: Celery extract capable of decreasing levels of 8-OHdG and the number of foam cells in Wistar rats induced with adrenaline and high-fat diet.Agnes BudiartoTaufiqurrachman NasihunAtina HussaanaUniversitas Sultan Agung Semarangarticlecelery8-ohdgfoam cellMedicine (General)R5-920ENSains Medika, Vol 8, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	celery 8-ohdg foam cell Medicine (General) R5-920
spellingShingle	celery 8-ohdg foam cell Medicine (General) R5-920 Agnes Budiarto Taufiqurrachman Nasihun Atina Hussaana Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
description	BACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract. OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2’-Deoxy Guanosine) and the number of foam cell. METHODS: Experimental studies with post-test only control group design used Wistar rats, administered with adrenaline and high-fat diet for hyperlipidemic induction.Total of 35 rats were divided into 5 groups: negative controls (Neg-G); positive control treated with simvastatin (Nor-G); ES25-G,ES50-G, and ES75-G group were treated with 25 mg/200 g BW, 50 mg/200 g BW, and 75 mg/200 g BW celery extract, respectively. The treatment was given for 30 days. The 8-OHdG level was determined by ELISA, whereas the number of foam cells was determined by histopathologic preparations with HE staining. Data analysis was conducted by Kruskal-Wallis and Mann-Whitney. RESULTS: Mann-Whitney analysis showed levels of 8-OHdG in ES-25-G 12.13±0.21, ES-50-G 7.23±0.25, ES-75-G 4.41±0.45 significantly lower than the Nor-G 14.30±0.66, respectively p<0.001. The number of foam cells in the ES-25-G was 7.57±0.53, ES-50-G 6.57±0.79, ES-75-G 3.43±0.53,significantly lower than the Neg-G 13.57±1.27, respectively p<0.001. CONCLUSION: Celery extract capable of decreasing levels of 8-OHdG and the number of foam cells in Wistar rats induced with adrenaline and high-fat diet.
format	article
author	Agnes Budiarto Taufiqurrachman Nasihun Atina Hussaana
author_facet	Agnes Budiarto Taufiqurrachman Nasihun Atina Hussaana
author_sort	Agnes Budiarto
title	Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
title_short	Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
title_full	Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
title_fullStr	Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
title_full_unstemmed	Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet
title_sort	effect of celery extract administration on 8-ohdg and number of foam cell in wistar strain rats with a high-fat diet
publisher	Universitas Sultan Agung Semarang
publishDate	2018
url	https://doaj.org/article/1aa8bd6961e74bba87b358b662980255
work_keys_str_mv	AT agnesbudiarto effectofceleryextractadministrationon8ohdgandnumberoffoamcellinwistarstrainratswithahighfatdiet AT taufiqurrachmannasihun effectofceleryextractadministrationon8ohdgandnumberoffoamcellinwistarstrainratswithahighfatdiet AT atinahussaana effectofceleryextractadministrationon8ohdgandnumberoffoamcellinwistarstrainratswithahighfatdiet
_version_	1718413482693492736

Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet

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