Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations
Recently, the design and development of nanozyme-based logic gates have received much attention. In this work, by engineering the stability of the nanozyme-catalyzed product, we demonstrated that the chromogenic system of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) can act as a visual output signal for...
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2021
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oai:doaj.org-article:1aaaf3974a3e4b398861b412536d74b62021-11-11T18:29:49ZEngineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations10.3390/molecules262164941420-3049https://doaj.org/article/1aaaf3974a3e4b398861b412536d74b62021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6494https://doaj.org/toc/1420-3049Recently, the design and development of nanozyme-based logic gates have received much attention. In this work, by engineering the stability of the nanozyme-catalyzed product, we demonstrated that the chromogenic system of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) can act as a visual output signal for constructing various Boolean logic operations. Specifically, cerium oxide or ferroferric oxide-based nanozymes can catalyze the oxidation of colorless TMB to a blue color product (oxTMB). The blue-colored solution of oxTMB could become colorless by some reductants, including the reduced transition state of glucose oxidase and xanthine oxidase. As a result, by combining biocatalytic reactions, the color change of oxTMB could be controlled logically. In our logic systems, glucose oxidase, β-galactosidase, and xanthine oxidase acted as inputs, and the state of oxTMB solution was used as an output. The logic operation produced a colored solution as the readout signal, which was easily distinguished with the naked eye. More importantly, the study of such a decolorization process allows the transformation of previously designed AND and OR logic gates into NAND and NOR gates. We propose that this work may push forward the design of novel nanozyme-based biological gates and help us further understand complex physiological pathways in living systems.Lianlian FuDeshuai YuDijuan ZouHao QianYouhui LinMDPI AGarticlenanozymelogic gatecolorimetric reactiondecolorizationproduct stabilityOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6494, p 6494 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
nanozyme logic gate colorimetric reaction decolorization product stability Organic chemistry QD241-441 |
| spellingShingle |
nanozyme logic gate colorimetric reaction decolorization product stability Organic chemistry QD241-441 Lianlian Fu Deshuai Yu Dijuan Zou Hao Qian Youhui Lin Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| description |
Recently, the design and development of nanozyme-based logic gates have received much attention. In this work, by engineering the stability of the nanozyme-catalyzed product, we demonstrated that the chromogenic system of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) can act as a visual output signal for constructing various Boolean logic operations. Specifically, cerium oxide or ferroferric oxide-based nanozymes can catalyze the oxidation of colorless TMB to a blue color product (oxTMB). The blue-colored solution of oxTMB could become colorless by some reductants, including the reduced transition state of glucose oxidase and xanthine oxidase. As a result, by combining biocatalytic reactions, the color change of oxTMB could be controlled logically. In our logic systems, glucose oxidase, β-galactosidase, and xanthine oxidase acted as inputs, and the state of oxTMB solution was used as an output. The logic operation produced a colored solution as the readout signal, which was easily distinguished with the naked eye. More importantly, the study of such a decolorization process allows the transformation of previously designed AND and OR logic gates into NAND and NOR gates. We propose that this work may push forward the design of novel nanozyme-based biological gates and help us further understand complex physiological pathways in living systems. |
| format |
article |
| author |
Lianlian Fu Deshuai Yu Dijuan Zou Hao Qian Youhui Lin |
| author_facet |
Lianlian Fu Deshuai Yu Dijuan Zou Hao Qian Youhui Lin |
| author_sort |
Lianlian Fu |
| title |
Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| title_short |
Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| title_full |
Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| title_fullStr |
Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| title_full_unstemmed |
Engineering the Stability of Nanozyme-Catalyzed Product for Colorimetric Logic Gate Operations |
| title_sort |
engineering the stability of nanozyme-catalyzed product for colorimetric logic gate operations |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/1aaaf3974a3e4b398861b412536d74b6 |
| work_keys_str_mv |
AT lianlianfu engineeringthestabilityofnanozymecatalyzedproductforcolorimetriclogicgateoperations AT deshuaiyu engineeringthestabilityofnanozymecatalyzedproductforcolorimetriclogicgateoperations AT dijuanzou engineeringthestabilityofnanozymecatalyzedproductforcolorimetriclogicgateoperations AT haoqian engineeringthestabilityofnanozymecatalyzedproductforcolorimetriclogicgateoperations AT youhuilin engineeringthestabilityofnanozymecatalyzedproductforcolorimetriclogicgateoperations |
| _version_ |
1718431809136492544 |