Patterning bacterial communities on epithelial cells.

Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterp...

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Autores principales:	Mohammed Dwidar, Brendan M Leung, Toshiyuki Yaguchi, Shuichi Takayama, Robert J Mitchell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ab56b66c5b3473ca5fdc2221b2a579d
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spelling	oai:doaj.org-article:1ab56b66c5b3473ca5fdc2221b2a579d2021-11-18T07:41:48ZPatterning bacterial communities on epithelial cells.1932-620310.1371/journal.pone.0067165https://doaj.org/article/1ab56b66c5b3473ca5fdc2221b2a579d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23785519/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibriobacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions.Mohammed DwidarBrendan M LeungToshiyuki YaguchiShuichi TakayamaRobert J MitchellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67165 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammed Dwidar Brendan M Leung Toshiyuki Yaguchi Shuichi Takayama Robert J Mitchell Patterning bacterial communities on epithelial cells.
description	Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibriobacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions.
format	article
author	Mohammed Dwidar Brendan M Leung Toshiyuki Yaguchi Shuichi Takayama Robert J Mitchell
author_facet	Mohammed Dwidar Brendan M Leung Toshiyuki Yaguchi Shuichi Takayama Robert J Mitchell
author_sort	Mohammed Dwidar
title	Patterning bacterial communities on epithelial cells.
title_short	Patterning bacterial communities on epithelial cells.
title_full	Patterning bacterial communities on epithelial cells.
title_fullStr	Patterning bacterial communities on epithelial cells.
title_full_unstemmed	Patterning bacterial communities on epithelial cells.
title_sort	patterning bacterial communities on epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1ab56b66c5b3473ca5fdc2221b2a579d
work_keys_str_mv	AT mohammeddwidar patterningbacterialcommunitiesonepithelialcells AT brendanmleung patterningbacterialcommunitiesonepithelialcells AT toshiyukiyaguchi patterningbacterialcommunitiesonepithelialcells AT shuichitakayama patterningbacterialcommunitiesonepithelialcells AT robertjmitchell patterningbacterialcommunitiesonepithelialcells
_version_	1718423081311010816

Patterning bacterial communities on epithelial cells.

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