High-throughput sequencing for species authentication and contamination detection of 63 cell lines

Abstract Cell lines are widely used in research and for diagnostic tests and are often shared between laboratories. Lack of cell line authentication can result in the use of contaminated or misidentified cell lines, potentially affecting the results from research and diagnostic activities. Cell line...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Oliver Lung, Rebecca Candlish, Michelle Nebroski, Peter Kruckiewicz, Cody Buchanan, Mariko Moniwa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ab6efbf42494a7d978e8bec9d214b09
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1ab6efbf42494a7d978e8bec9d214b09
record_format	dspace
spelling	oai:doaj.org-article:1ab6efbf42494a7d978e8bec9d214b092021-11-08T10:50:17ZHigh-throughput sequencing for species authentication and contamination detection of 63 cell lines10.1038/s41598-021-00779-52045-2322https://doaj.org/article/1ab6efbf42494a7d978e8bec9d214b092021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00779-5https://doaj.org/toc/2045-2322Abstract Cell lines are widely used in research and for diagnostic tests and are often shared between laboratories. Lack of cell line authentication can result in the use of contaminated or misidentified cell lines, potentially affecting the results from research and diagnostic activities. Cell line authentication and contamination detection based on metagenomic high-throughput sequencing (HTS) was tested on DNA and RNA from 63 cell lines available at the Canadian Food Inspection Agency’s National Centre for Foreign Animal Disease. Through sequence comparison of the cytochrome c oxidase subunit 1 (COX1) gene, the species identity of 53 cell lines was confirmed, and eight cell lines were found to show a greater pairwise nucleotide identity in the COX1 sequence of a different species within the same expected genus. Two cell lines, LFBK-αvβ6 and SCP-HS, were determined to be composed of cells from a different species and genus. Mycoplasma contamination was not detected in any cell lines. However, several expected and unexpected viral sequences were detected, including part of the classical swine fever virus genome in the IB-RS-2 Clone D10 cell line. Metagenomics-based HTS is a useful laboratory QA tool for cell line authentication and contamination detection that should be conducted regularly.Oliver LungRebecca CandlishMichelle NebroskiPeter KruckiewiczCody BuchananMariko MoniwaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliver Lung Rebecca Candlish Michelle Nebroski Peter Kruckiewicz Cody Buchanan Mariko Moniwa High-throughput sequencing for species authentication and contamination detection of 63 cell lines
description	Abstract Cell lines are widely used in research and for diagnostic tests and are often shared between laboratories. Lack of cell line authentication can result in the use of contaminated or misidentified cell lines, potentially affecting the results from research and diagnostic activities. Cell line authentication and contamination detection based on metagenomic high-throughput sequencing (HTS) was tested on DNA and RNA from 63 cell lines available at the Canadian Food Inspection Agency’s National Centre for Foreign Animal Disease. Through sequence comparison of the cytochrome c oxidase subunit 1 (COX1) gene, the species identity of 53 cell lines was confirmed, and eight cell lines were found to show a greater pairwise nucleotide identity in the COX1 sequence of a different species within the same expected genus. Two cell lines, LFBK-αvβ6 and SCP-HS, were determined to be composed of cells from a different species and genus. Mycoplasma contamination was not detected in any cell lines. However, several expected and unexpected viral sequences were detected, including part of the classical swine fever virus genome in the IB-RS-2 Clone D10 cell line. Metagenomics-based HTS is a useful laboratory QA tool for cell line authentication and contamination detection that should be conducted regularly.
format	article
author	Oliver Lung Rebecca Candlish Michelle Nebroski Peter Kruckiewicz Cody Buchanan Mariko Moniwa
author_facet	Oliver Lung Rebecca Candlish Michelle Nebroski Peter Kruckiewicz Cody Buchanan Mariko Moniwa
author_sort	Oliver Lung
title	High-throughput sequencing for species authentication and contamination detection of 63 cell lines
title_short	High-throughput sequencing for species authentication and contamination detection of 63 cell lines
title_full	High-throughput sequencing for species authentication and contamination detection of 63 cell lines
title_fullStr	High-throughput sequencing for species authentication and contamination detection of 63 cell lines
title_full_unstemmed	High-throughput sequencing for species authentication and contamination detection of 63 cell lines
title_sort	high-throughput sequencing for species authentication and contamination detection of 63 cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ab6efbf42494a7d978e8bec9d214b09
work_keys_str_mv	AT oliverlung highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines AT rebeccacandlish highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines AT michellenebroski highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines AT peterkruckiewicz highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines AT codybuchanan highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines AT marikomoniwa highthroughputsequencingforspeciesauthenticationandcontaminationdetectionof63celllines
_version_	1718442593148207104

High-throughput sequencing for species authentication and contamination detection of 63 cell lines

Ejemplares similares