Long non-coding RNA PARTICLE bridges histone and DNA methylation

Abstract PARTICLE (Gene PARTICL- ‘Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting...

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Autores principales:	Valerie Bríd O’Leary, Sarah Hain, Doris Maugg, Jan Smida, Omid Azimzadeh, Soile Tapio, Saak Victor Ovsepian, Michael John Atkinson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ac21c1020d642bd9df0881be713188f
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spelling	oai:doaj.org-article:1ac21c1020d642bd9df0881be713188f2021-12-02T11:41:18ZLong non-coding RNA PARTICLE bridges histone and DNA methylation10.1038/s41598-017-01875-12045-2322https://doaj.org/article/1ac21c1020d642bd9df0881be713188f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01875-1https://doaj.org/toc/2045-2322Abstract PARTICLE (Gene PARTICL- ‘Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.Valerie Bríd O’LearySarah HainDoris MauggJan SmidaOmid AzimzadehSoile TapioSaak Victor OvsepianMichael John AtkinsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valerie Bríd O’Leary Sarah Hain Doris Maugg Jan Smida Omid Azimzadeh Soile Tapio Saak Victor Ovsepian Michael John Atkinson Long non-coding RNA PARTICLE bridges histone and DNA methylation
description	Abstract PARTICLE (Gene PARTICL- ‘Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.
format	article
author	Valerie Bríd O’Leary Sarah Hain Doris Maugg Jan Smida Omid Azimzadeh Soile Tapio Saak Victor Ovsepian Michael John Atkinson
author_facet	Valerie Bríd O’Leary Sarah Hain Doris Maugg Jan Smida Omid Azimzadeh Soile Tapio Saak Victor Ovsepian Michael John Atkinson
author_sort	Valerie Bríd O’Leary
title	Long non-coding RNA PARTICLE bridges histone and DNA methylation
title_short	Long non-coding RNA PARTICLE bridges histone and DNA methylation
title_full	Long non-coding RNA PARTICLE bridges histone and DNA methylation
title_fullStr	Long non-coding RNA PARTICLE bridges histone and DNA methylation
title_full_unstemmed	Long non-coding RNA PARTICLE bridges histone and DNA methylation
title_sort	long non-coding rna particle bridges histone and dna methylation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1ac21c1020d642bd9df0881be713188f
work_keys_str_mv	AT valeriebridoleary longnoncodingrnaparticlebridgeshistoneanddnamethylation AT sarahhain longnoncodingrnaparticlebridgeshistoneanddnamethylation AT dorismaugg longnoncodingrnaparticlebridgeshistoneanddnamethylation AT jansmida longnoncodingrnaparticlebridgeshistoneanddnamethylation AT omidazimzadeh longnoncodingrnaparticlebridgeshistoneanddnamethylation AT soiletapio longnoncodingrnaparticlebridgeshistoneanddnamethylation AT saakvictorovsepian longnoncodingrnaparticlebridgeshistoneanddnamethylation AT michaeljohnatkinson longnoncodingrnaparticlebridgeshistoneanddnamethylation
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Long non-coding RNA PARTICLE bridges histone and DNA methylation

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