Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis

Angiosperm flowers are the most complex organs that plants generate, and in their center, the gynoecium forms, assuring sexual reproduction. Gynoecium development requires tight regulation of developmental regulators across time and tissues. How simple on and off regulation of gene expression is ach...

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Autores principales:	Thomas Gross, Annette Becker
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Arabidopsis thaliana</i> carpel development complex expression pattern transcriptional regulation transcription factor Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1ad706c8b1dd47a1a370840417e7b80d
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spelling	oai:doaj.org-article:1ad706c8b1dd47a1a370840417e7b80d2021-11-25T17:40:25ZTranscription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis10.3390/genes121116632073-4425https://doaj.org/article/1ad706c8b1dd47a1a370840417e7b80d2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1663https://doaj.org/toc/2073-4425Angiosperm flowers are the most complex organs that plants generate, and in their center, the gynoecium forms, assuring sexual reproduction. Gynoecium development requires tight regulation of developmental regulators across time and tissues. How simple on and off regulation of gene expression is achieved in plants was described previously, but molecular mechanisms generating complex expression patterns remain unclear. We use the gynoecium developmental regulator <i>CRABS CLAW</i> (<i>CRC</i>) to study factors contributing to its sophisticated expression pattern. We combine in silico promoter analyses, global TF-DNA interaction screens, and mutant analyses. We find that miRNA action, DNA methylation, and chromatin remodeling do not contribute substantially to <i>CRC</i> regulation. However, 119 TFs, including SEP3, ETT, CAL, FUL, NGA2, and JAG bind to the <i>CRC</i> promoter in yeast. These TFs finetune transcript abundance as homodimers by transcriptional activation. Interestingly, temporal–spatial aspects of expression regulation may be under the control of redundantly acting genes and require higher order complex formation at TF binding sites. Our work shows that endogenous regulation of complex expression pattern requires orchestrated transcription factor action on several conserved promotor sites covering almost 4 kb in length. Our results highlight the utility of comprehensive regulators screens directly linking transcriptional regulators with their targets.Thomas GrossAnnette BeckerMDPI AGarticle<i>Arabidopsis thaliana</i>carpel developmentcomplex expression patterntranscriptional regulationtranscription factorGeneticsQH426-470ENGenes, Vol 12, Iss 1663, p 1663 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Arabidopsis thaliana</i> carpel development complex expression pattern transcriptional regulation transcription factor Genetics QH426-470
spellingShingle	<i>Arabidopsis thaliana</i> carpel development complex expression pattern transcriptional regulation transcription factor Genetics QH426-470 Thomas Gross Annette Becker Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
description	Angiosperm flowers are the most complex organs that plants generate, and in their center, the gynoecium forms, assuring sexual reproduction. Gynoecium development requires tight regulation of developmental regulators across time and tissues. How simple on and off regulation of gene expression is achieved in plants was described previously, but molecular mechanisms generating complex expression patterns remain unclear. We use the gynoecium developmental regulator <i>CRABS CLAW</i> (<i>CRC</i>) to study factors contributing to its sophisticated expression pattern. We combine in silico promoter analyses, global TF-DNA interaction screens, and mutant analyses. We find that miRNA action, DNA methylation, and chromatin remodeling do not contribute substantially to <i>CRC</i> regulation. However, 119 TFs, including SEP3, ETT, CAL, FUL, NGA2, and JAG bind to the <i>CRC</i> promoter in yeast. These TFs finetune transcript abundance as homodimers by transcriptional activation. Interestingly, temporal–spatial aspects of expression regulation may be under the control of redundantly acting genes and require higher order complex formation at TF binding sites. Our work shows that endogenous regulation of complex expression pattern requires orchestrated transcription factor action on several conserved promotor sites covering almost 4 kb in length. Our results highlight the utility of comprehensive regulators screens directly linking transcriptional regulators with their targets.
format	article
author	Thomas Gross Annette Becker
author_facet	Thomas Gross Annette Becker
author_sort	Thomas Gross
title	Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
title_short	Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
title_full	Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
title_fullStr	Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
title_full_unstemmed	Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis
title_sort	transcription factor action orchestrates the complex expression pattern of crabs claw in arabidopsis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1ad706c8b1dd47a1a370840417e7b80d
work_keys_str_mv	AT thomasgross transcriptionfactoractionorchestratesthecomplexexpressionpatternofcrabsclawinarabidopsis AT annettebecker transcriptionfactoractionorchestratesthecomplexexpressionpatternofcrabsclawinarabidopsis
_version_	1718412091227897856

Transcription Factor Action Orchestrates the Complex Expression Pattern of CRABS CLAW in Arabidopsis

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