Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.

Estrogen plays an important role in the maintenance of bone formation, and deficiency in the production of estrogen is directly linked to postmenopausal osteoporosis. To date, the underlying mechanisms of estrogen-mediated osteogenic differentiation are not well understood. In this study, a pluripot...

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Autores principales:	Jinlan Gao, Qi Liu, Xing Liu, Chunyan Ji, Shengqiang Qu, Shusen Wang, Yang Luo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ad949283bde4278bd3b945c5355fcb5
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spelling	oai:doaj.org-article:1ad949283bde4278bd3b945c5355fcb52021-11-18T08:29:57ZCyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.1932-620310.1371/journal.pone.0089884https://doaj.org/article/1ad949283bde4278bd3b945c5355fcb52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24595300/?tool=EBIhttps://doaj.org/toc/1932-6203Estrogen plays an important role in the maintenance of bone formation, and deficiency in the production of estrogen is directly linked to postmenopausal osteoporosis. To date, the underlying mechanisms of estrogen-mediated osteogenic differentiation are not well understood. In this study, a pluripotent mesenchymal precursor cell line C2C12 was used to induce osteogenic differentiation and subjected to detection of gene expressions or to manipulation of cyclin G2 expressions. C57BL/6 mice were used to generate bilateral ovariectomized and sham-operated mice for analysis of bone mineral density and protein expression. We identified cyclin G2, an unconventional member of cyclin, is involved in osteoblast differentiation regulated by estrogen in vivo and in vitro. In addition, the data showed that ectopic expression of cyclin G2 suppressed expression of osteoblast transcription factor Runx2 and osteogenic differentiation marker genes, as well as ALP activity and in vitro extracellular matrix mineralization. Mechanistically, Wnt/β-catenin signaling pathway is essential for cyclin G2 to inhibit osteogenic differentiation. To the best of our knowledge, the current study presents the first evidence that cyclin G2 serves as a negative regulator of both osteogenesis and Wnt/β-catenin signaling. Most importantly, the basal and 17β-estradiol-induced osteogenic differentiation was restored by overexpression of cyclin G2. These results taken together suggest that cyclin G2 may function as an endogenous suppressor of estrogen-induced osteogenic differentiation through inhibition of Wnt/β-catenin signaling.Jinlan GaoQi LiuXing LiuChunyan JiShengqiang QuShusen WangYang LuoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e89884 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinlan Gao Qi Liu Xing Liu Chunyan Ji Shengqiang Qu Shusen Wang Yang Luo Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
description	Estrogen plays an important role in the maintenance of bone formation, and deficiency in the production of estrogen is directly linked to postmenopausal osteoporosis. To date, the underlying mechanisms of estrogen-mediated osteogenic differentiation are not well understood. In this study, a pluripotent mesenchymal precursor cell line C2C12 was used to induce osteogenic differentiation and subjected to detection of gene expressions or to manipulation of cyclin G2 expressions. C57BL/6 mice were used to generate bilateral ovariectomized and sham-operated mice for analysis of bone mineral density and protein expression. We identified cyclin G2, an unconventional member of cyclin, is involved in osteoblast differentiation regulated by estrogen in vivo and in vitro. In addition, the data showed that ectopic expression of cyclin G2 suppressed expression of osteoblast transcription factor Runx2 and osteogenic differentiation marker genes, as well as ALP activity and in vitro extracellular matrix mineralization. Mechanistically, Wnt/β-catenin signaling pathway is essential for cyclin G2 to inhibit osteogenic differentiation. To the best of our knowledge, the current study presents the first evidence that cyclin G2 serves as a negative regulator of both osteogenesis and Wnt/β-catenin signaling. Most importantly, the basal and 17β-estradiol-induced osteogenic differentiation was restored by overexpression of cyclin G2. These results taken together suggest that cyclin G2 may function as an endogenous suppressor of estrogen-induced osteogenic differentiation through inhibition of Wnt/β-catenin signaling.
format	article
author	Jinlan Gao Qi Liu Xing Liu Chunyan Ji Shengqiang Qu Shusen Wang Yang Luo
author_facet	Jinlan Gao Qi Liu Xing Liu Chunyan Ji Shengqiang Qu Shusen Wang Yang Luo
author_sort	Jinlan Gao
title	Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
title_short	Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
title_full	Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
title_fullStr	Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
title_full_unstemmed	Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.
title_sort	cyclin g2 suppresses estrogen-mediated osteogenesis through inhibition of wnt/β-catenin signaling.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1ad949283bde4278bd3b945c5355fcb5
work_keys_str_mv	AT jinlangao cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT qiliu cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT xingliu cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT chunyanji cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT shengqiangqu cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT shusenwang cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling AT yangluo cycling2suppressesestrogenmediatedosteogenesisthroughinhibitionofwntbcateninsignaling
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Cyclin G2 suppresses estrogen-mediated osteogenesis through inhibition of Wnt/β-catenin signaling.

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