Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community
Abstract Conjunctival pneumococcal serotypes among members of a community have not been investigated well. We determined the prevalence and association of Streptococcus pneumoniae in the nasopharynx and conjunctiva among children in a community before pneumococcal conjugate vaccine introduction. In...
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2021
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oai:doaj.org-article:1ae1677194cc46fcb8051a43346cd80c2021-12-02T14:12:07ZPrevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community10.1038/s41598-020-79175-42045-2322https://doaj.org/article/1ae1677194cc46fcb8051a43346cd80c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79175-4https://doaj.org/toc/2045-2322Abstract Conjunctival pneumococcal serotypes among members of a community have not been investigated well. We determined the prevalence and association of Streptococcus pneumoniae in the nasopharynx and conjunctiva among children in a community before pneumococcal conjugate vaccine introduction. In October 2016, conjunctival and nasopharyngeal swabs were collected from children (< 24 months old) and nasopharyngeal swabs from mothers in Nha Trang, Vietnam. Quantitative lytA PCR and DNA microarray were performed to detect and serotype S. pneumoniae. The association between S. pneumoniae in the nasopharynx and conjunctiva was evaluated using multivariable logistic regression model. Among 698 children, 62 (8.9%, 95% CI 6.9–11.2%) were positive for S. pneumoniae in the conjunctiva. Non-encapsulated S. pneumoniae were most commonly identified, followed by serotypes 6A, 6B, and 14. Nasopharyngeal and conjunctival detection were positively associated (aOR 47.30, 95% CI 24.07–92.97). Low birth-weight, day-care attendance, and recent eye symptoms were independently associated with S. pneumoniae detection in the conjunctiva (aOR 11.14, 95% CI 3.76–32.98, aOR 2.19, 95% CI 1.45–3.31, and aOR 3.59, 95% CI 2.21–5.84, respectively). Serotypes and genotypes in the conjunctiva and nasopharynx matched in 87% of the children. Three mothers’ nasopharyngeal pneumococcal samples had matched serotype and genotype with their child’s in the conjunctiva and nasopharynx. S. pneumoniae presence in nasopharynx and conjunctiva were strongly associated. The high concordance of serotypes suggests nasopharyngeal carriage may be a source of transmission to the conjunctiva.Yasser Helmy MohamedMichiko ToizumiMasafumi UematsuHien-Anh Thi NguyenLien Thuy LeMizuki TakegataChihiro IwasakiNoriko KitamuraMonica L. NationEileen M. DunneJason HindsHung Thai DoMai Quang VienCatherine SatzkeStefan FlascheKim MulhollandDuc-Anh DangTakashi KitaokaLay-Myint YoshidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Yasser Helmy Mohamed Michiko Toizumi Masafumi Uematsu Hien-Anh Thi Nguyen Lien Thuy Le Mizuki Takegata Chihiro Iwasaki Noriko Kitamura Monica L. Nation Eileen M. Dunne Jason Hinds Hung Thai Do Mai Quang Vien Catherine Satzke Stefan Flasche Kim Mulholland Duc-Anh Dang Takashi Kitaoka Lay-Myint Yoshida Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| description |
Abstract Conjunctival pneumococcal serotypes among members of a community have not been investigated well. We determined the prevalence and association of Streptococcus pneumoniae in the nasopharynx and conjunctiva among children in a community before pneumococcal conjugate vaccine introduction. In October 2016, conjunctival and nasopharyngeal swabs were collected from children (< 24 months old) and nasopharyngeal swabs from mothers in Nha Trang, Vietnam. Quantitative lytA PCR and DNA microarray were performed to detect and serotype S. pneumoniae. The association between S. pneumoniae in the nasopharynx and conjunctiva was evaluated using multivariable logistic regression model. Among 698 children, 62 (8.9%, 95% CI 6.9–11.2%) were positive for S. pneumoniae in the conjunctiva. Non-encapsulated S. pneumoniae were most commonly identified, followed by serotypes 6A, 6B, and 14. Nasopharyngeal and conjunctival detection were positively associated (aOR 47.30, 95% CI 24.07–92.97). Low birth-weight, day-care attendance, and recent eye symptoms were independently associated with S. pneumoniae detection in the conjunctiva (aOR 11.14, 95% CI 3.76–32.98, aOR 2.19, 95% CI 1.45–3.31, and aOR 3.59, 95% CI 2.21–5.84, respectively). Serotypes and genotypes in the conjunctiva and nasopharynx matched in 87% of the children. Three mothers’ nasopharyngeal pneumococcal samples had matched serotype and genotype with their child’s in the conjunctiva and nasopharynx. S. pneumoniae presence in nasopharynx and conjunctiva were strongly associated. The high concordance of serotypes suggests nasopharyngeal carriage may be a source of transmission to the conjunctiva. |
| format |
article |
| author |
Yasser Helmy Mohamed Michiko Toizumi Masafumi Uematsu Hien-Anh Thi Nguyen Lien Thuy Le Mizuki Takegata Chihiro Iwasaki Noriko Kitamura Monica L. Nation Eileen M. Dunne Jason Hinds Hung Thai Do Mai Quang Vien Catherine Satzke Stefan Flasche Kim Mulholland Duc-Anh Dang Takashi Kitaoka Lay-Myint Yoshida |
| author_facet |
Yasser Helmy Mohamed Michiko Toizumi Masafumi Uematsu Hien-Anh Thi Nguyen Lien Thuy Le Mizuki Takegata Chihiro Iwasaki Noriko Kitamura Monica L. Nation Eileen M. Dunne Jason Hinds Hung Thai Do Mai Quang Vien Catherine Satzke Stefan Flasche Kim Mulholland Duc-Anh Dang Takashi Kitaoka Lay-Myint Yoshida |
| author_sort |
Yasser Helmy Mohamed |
| title |
Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| title_short |
Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| title_full |
Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| title_fullStr |
Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| title_full_unstemmed |
Prevalence of Streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a Vietnamese community |
| title_sort |
prevalence of streptococcus pneumoniae in conjunctival flora and association with nasopharyngeal carriage among children in a vietnamese community |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/1ae1677194cc46fcb8051a43346cd80c |
| work_keys_str_mv |
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