Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.

A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in min...

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Autores principales:	Saurabh Gangola, Samiksha Joshi, Saurabh Kumar, Barkha Sharma, Anita Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ae1d6f0485c420493fff1e8531c9b10
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spelling	oai:doaj.org-article:1ae1d6f0485c420493fff1e8531c9b102021-12-02T20:18:10ZDifferential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.1932-620310.1371/journal.pone.0253106https://doaj.org/article/1ae1d6f0485c420493fff1e8531c9b102021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253106https://doaj.org/toc/1932-6203A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in gas chromatography mass spectrometry (GC-MS) analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (Accession ID: MH341691). Stress responsive and catabolic/pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzymatic kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions which suggested that the production of enzyme was induced significantly in pesticide stress (163 μg.μL-1) as compare to normal conditions(29 μg.μL-1) while the Km value was decreased in pesticides stress condition (Km = 10.57 mM) and increases in normal condition (Km = 14.33 mM).Amplification of laccase gene showed a major band of 1200bp. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).Saurabh GangolaSamiksha JoshiSaurabh KumarBarkha SharmaAnita SharmaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0253106 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saurabh Gangola Samiksha Joshi Saurabh Kumar Barkha Sharma Anita Sharma Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
description	A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in gas chromatography mass spectrometry (GC-MS) analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (Accession ID: MH341691). Stress responsive and catabolic/pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzymatic kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions which suggested that the production of enzyme was induced significantly in pesticide stress (163 μg.μL-1) as compare to normal conditions(29 μg.μL-1) while the Km value was decreased in pesticides stress condition (Km = 10.57 mM) and increases in normal condition (Km = 14.33 mM).Amplification of laccase gene showed a major band of 1200bp. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).
format	article
author	Saurabh Gangola Samiksha Joshi Saurabh Kumar Barkha Sharma Anita Sharma
author_facet	Saurabh Gangola Samiksha Joshi Saurabh Kumar Barkha Sharma Anita Sharma
author_sort	Saurabh Gangola
title	Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
title_short	Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
title_full	Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
title_fullStr	Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
title_full_unstemmed	Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.
title_sort	differential proteomic analysis under pesticides stress and normal conditions in bacillus cereus 2d.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1ae1d6f0485c420493fff1e8531c9b10
work_keys_str_mv	AT saurabhgangola differentialproteomicanalysisunderpesticidesstressandnormalconditionsinbacilluscereus2d AT samikshajoshi differentialproteomicanalysisunderpesticidesstressandnormalconditionsinbacilluscereus2d AT saurabhkumar differentialproteomicanalysisunderpesticidesstressandnormalconditionsinbacilluscereus2d AT barkhasharma differentialproteomicanalysisunderpesticidesstressandnormalconditionsinbacilluscereus2d AT anitasharma differentialproteomicanalysisunderpesticidesstressandnormalconditionsinbacilluscereus2d
_version_	1718374271236964352

Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D.

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