Candida albicans biofilm on titanium: effect of peroxidase precoating

Mohamed Ahariz1, Philippe Courtois1,21Laboratory of Experimental Hormonology, Universit&eacute; Libre de Bruxelles, Brussels, 2UER de Biologie M&eacute;dicale, Haute Ecole Francisco Ferrer, Brussels, BelgiumAbstract: The present study aimed to document Candida albicans biofilm develo...

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Autores principales:	Mohamed Ahariz, Philippe Courtois
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/1ae4f386e6fd4cc286f4248202e85414
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spelling	oai:doaj.org-article:1ae4f386e6fd4cc286f4248202e854142021-12-02T09:11:00ZCandida albicans biofilm on titanium: effect of peroxidase precoating1179-1470https://doaj.org/article/1ae4f386e6fd4cc286f4248202e854142010-08-01T00:00:00Zhttp://www.dovepress.com/candida-albicans-biofilm-on-titanium-effect-of-peroxidase-precoating-a4970https://doaj.org/toc/1179-1470Mohamed Ahariz1, Philippe Courtois1,21Laboratory of Experimental Hormonology, Universit&eacute; Libre de Bruxelles, Brussels, 2UER de Biologie M&eacute;dicale, Haute Ecole Francisco Ferrer, Brussels, BelgiumAbstract: The present study aimed to document Candida albicans biofilm development on titanium and its modulation by a peroxidase-precoated material which can generate antimicrobials, such as hypoiodite or hypothiocyanite, from hydrogen peroxide, iodide, or thiocyanate. For this purpose, titanium (powder or foil) was suspended in Sabouraud liquid medium inoculated with C. albicans ATCC10231. After continuous stirring for 2&ndash;21 days at room temperature, the supernatant was monitored by turbidimetry at 600 nm and titanium washed three times in sterile Sabouraud broth. Using the tetrazolium salt MTT-formazan assay, the titanium-adherent fungal biomass was measured as 7.50 &plusmn; 0.60 &times; 106 blastoconidia per gram of titanium powder (n = 30) and 0.50 &plusmn; 0.04 &times; 106 blastoconidia per cm&sup2; of titanium foil (n = 12). The presence of yeast on the surface of titanium was confirmed by microscopy both on fresh preparations and after calcofluor white staining. However, in the presence of peroxidase systems (lactoperoxidase with substrates such as hydrogen peroxide donor, iodide, or thiocyanate), Candida growth in both planktonic and attached phases appeared to be inhibited. Moreover, this study demonstrates the possible partition of peroxidase systems between titanium material (peroxidase-precoated) and liquid environment (containing peroxidase substrates) to limit C. albicans biofilm formation.Keywords: adhesion, material, oral, yeast Mohamed AharizPhilippe CourtoisDove Medical PressarticleMedical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol 2010, Iss default, Pp 33-40 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medical technology R855-855.5
spellingShingle	Medical technology R855-855.5 Mohamed Ahariz Philippe Courtois Candida albicans biofilm on titanium: effect of peroxidase precoating
description	Mohamed Ahariz1, Philippe Courtois1,21Laboratory of Experimental Hormonology, Universit&eacute; Libre de Bruxelles, Brussels, 2UER de Biologie M&eacute;dicale, Haute Ecole Francisco Ferrer, Brussels, BelgiumAbstract: The present study aimed to document Candida albicans biofilm development on titanium and its modulation by a peroxidase-precoated material which can generate antimicrobials, such as hypoiodite or hypothiocyanite, from hydrogen peroxide, iodide, or thiocyanate. For this purpose, titanium (powder or foil) was suspended in Sabouraud liquid medium inoculated with C. albicans ATCC10231. After continuous stirring for 2&ndash;21 days at room temperature, the supernatant was monitored by turbidimetry at 600 nm and titanium washed three times in sterile Sabouraud broth. Using the tetrazolium salt MTT-formazan assay, the titanium-adherent fungal biomass was measured as 7.50 &plusmn; 0.60 &times; 106 blastoconidia per gram of titanium powder (n = 30) and 0.50 &plusmn; 0.04 &times; 106 blastoconidia per cm&sup2; of titanium foil (n = 12). The presence of yeast on the surface of titanium was confirmed by microscopy both on fresh preparations and after calcofluor white staining. However, in the presence of peroxidase systems (lactoperoxidase with substrates such as hydrogen peroxide donor, iodide, or thiocyanate), Candida growth in both planktonic and attached phases appeared to be inhibited. Moreover, this study demonstrates the possible partition of peroxidase systems between titanium material (peroxidase-precoated) and liquid environment (containing peroxidase substrates) to limit C. albicans biofilm formation.Keywords: adhesion, material, oral, yeast
format	article
author	Mohamed Ahariz Philippe Courtois
author_facet	Mohamed Ahariz Philippe Courtois
author_sort	Mohamed Ahariz
title	Candida albicans biofilm on titanium: effect of peroxidase precoating
title_short	Candida albicans biofilm on titanium: effect of peroxidase precoating
title_full	Candida albicans biofilm on titanium: effect of peroxidase precoating
title_fullStr	Candida albicans biofilm on titanium: effect of peroxidase precoating
title_full_unstemmed	Candida albicans biofilm on titanium: effect of peroxidase precoating
title_sort	candida albicans biofilm on titanium: effect of peroxidase precoating
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/1ae4f386e6fd4cc286f4248202e85414
work_keys_str_mv	AT mohamedahariz candidaalbicansbiofilmontitaniumeffectofperoxidaseprecoating AT philippecourtois candidaalbicansbiofilmontitaniumeffectofperoxidaseprecoating
_version_	1718398169754107904

Candida albicans biofilm on titanium: effect of peroxidase precoating

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