Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33
Multidrug-resistant bacteria from different sources have been steadily emerging, and an increasing number of resistance mechanisms are being uncovered. In this work, we characterized a novel resistance gene named aac(2′)-If from an isolate of a novel Providencia species, Providencia wenzhouensis R33...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:1ae5e94ac1d546a4970a7490e8e2c4a12021-11-19T06:27:15ZIdentification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R331664-302X10.3389/fmicb.2021.711037https://doaj.org/article/1ae5e94ac1d546a4970a7490e8e2c4a12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.711037/fullhttps://doaj.org/toc/1664-302XMultidrug-resistant bacteria from different sources have been steadily emerging, and an increasing number of resistance mechanisms are being uncovered. In this work, we characterized a novel resistance gene named aac(2′)-If from an isolate of a novel Providencia species, Providencia wenzhouensis R33 (CCTCC AB 2021339). Susceptibility testing and enzyme kinetic parameter analysis were conducted to determine the function of the aminoglycoside 2′-N-acetyltransferase. Whole-genome sequencing and comparative genomic analysis were performed to elucidate the molecular characteristics of the genome and the genetic context of the resistance gene-related sequences. Among the functionally characterized resistance genes, AAC(2′)-If shares the highest amino acid sequence identity of 70.79% with AAC(2′)-Ia. AAC(2′)-If confers resistance to several aminoglycoside antibiotics, showing the highest resistance activity against ribostamycin and neomycin. The recombinant strain harboring aac(2′)-If (pUCP20-aac(2′)-If/DH5α) showed 256- and 128-fold increases in the minimum inhibitory concentration (MIC) levels to ribostamycin and neomycin, respectively, compared with those of the control strains (DH5α and pUCP20/DH5α). The results of the kinetic analysis of AAC(2′)-If were consistent with the MIC results of the cloned aac(2′)-If with the highest catalytic efficiency for ribostamycin (kcat/Km ratio = [3.72 ± 0.52] × 104 M–1⋅s–1). Whole-genome sequencing demonstrated that the aac(2′)-If gene was located on the chromosome with a relatively unique genetic environment. Identification of a novel aminoglycoside resistance gene in a strain of a novel Providencia species will help us find ways to elucidate the complexity of resistance mechanisms in the microbial population.Kexin ZhouKexin ZhouKexin ZhouJialei LiangJialei LiangJialei LiangXu DongXu DongPeiyao ZhangPeiyao ZhangChunlin FengChunlin FengWeina ShiWeina ShiMengdi GaoMengdi GaoQiaoling LiQiaoling LiQiaoling LiXueya ZhangXueya ZhangXueya ZhangJunwan LuJunwan LuXi LinXi LinKewei LiKewei LiHailin ZhangHailin ZhangMei ZhuQiyu BaoQiyu BaoQiyu BaoFrontiers Media S.A.articleProvidenciaaminoglycosideAAC(2cpsdummy′)resistancekinetic analysisMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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| collection |
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| language |
EN |
| topic |
Providencia aminoglycoside AAC(2cpsdummy′) resistance kinetic analysis Microbiology QR1-502 |
| spellingShingle |
Providencia aminoglycoside AAC(2cpsdummy′) resistance kinetic analysis Microbiology QR1-502 Kexin Zhou Kexin Zhou Kexin Zhou Jialei Liang Jialei Liang Jialei Liang Xu Dong Xu Dong Peiyao Zhang Peiyao Zhang Chunlin Feng Chunlin Feng Weina Shi Weina Shi Mengdi Gao Mengdi Gao Qiaoling Li Qiaoling Li Qiaoling Li Xueya Zhang Xueya Zhang Xueya Zhang Junwan Lu Junwan Lu Xi Lin Xi Lin Kewei Li Kewei Li Hailin Zhang Hailin Zhang Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| description |
Multidrug-resistant bacteria from different sources have been steadily emerging, and an increasing number of resistance mechanisms are being uncovered. In this work, we characterized a novel resistance gene named aac(2′)-If from an isolate of a novel Providencia species, Providencia wenzhouensis R33 (CCTCC AB 2021339). Susceptibility testing and enzyme kinetic parameter analysis were conducted to determine the function of the aminoglycoside 2′-N-acetyltransferase. Whole-genome sequencing and comparative genomic analysis were performed to elucidate the molecular characteristics of the genome and the genetic context of the resistance gene-related sequences. Among the functionally characterized resistance genes, AAC(2′)-If shares the highest amino acid sequence identity of 70.79% with AAC(2′)-Ia. AAC(2′)-If confers resistance to several aminoglycoside antibiotics, showing the highest resistance activity against ribostamycin and neomycin. The recombinant strain harboring aac(2′)-If (pUCP20-aac(2′)-If/DH5α) showed 256- and 128-fold increases in the minimum inhibitory concentration (MIC) levels to ribostamycin and neomycin, respectively, compared with those of the control strains (DH5α and pUCP20/DH5α). The results of the kinetic analysis of AAC(2′)-If were consistent with the MIC results of the cloned aac(2′)-If with the highest catalytic efficiency for ribostamycin (kcat/Km ratio = [3.72 ± 0.52] × 104 M–1⋅s–1). Whole-genome sequencing demonstrated that the aac(2′)-If gene was located on the chromosome with a relatively unique genetic environment. Identification of a novel aminoglycoside resistance gene in a strain of a novel Providencia species will help us find ways to elucidate the complexity of resistance mechanisms in the microbial population. |
| format |
article |
| author |
Kexin Zhou Kexin Zhou Kexin Zhou Jialei Liang Jialei Liang Jialei Liang Xu Dong Xu Dong Peiyao Zhang Peiyao Zhang Chunlin Feng Chunlin Feng Weina Shi Weina Shi Mengdi Gao Mengdi Gao Qiaoling Li Qiaoling Li Qiaoling Li Xueya Zhang Xueya Zhang Xueya Zhang Junwan Lu Junwan Lu Xi Lin Xi Lin Kewei Li Kewei Li Hailin Zhang Hailin Zhang Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao |
| author_facet |
Kexin Zhou Kexin Zhou Kexin Zhou Jialei Liang Jialei Liang Jialei Liang Xu Dong Xu Dong Peiyao Zhang Peiyao Zhang Chunlin Feng Chunlin Feng Weina Shi Weina Shi Mengdi Gao Mengdi Gao Qiaoling Li Qiaoling Li Qiaoling Li Xueya Zhang Xueya Zhang Xueya Zhang Junwan Lu Junwan Lu Xi Lin Xi Lin Kewei Li Kewei Li Hailin Zhang Hailin Zhang Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao |
| author_sort |
Kexin Zhou |
| title |
Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| title_short |
Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| title_full |
Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| title_fullStr |
Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| title_full_unstemmed |
Identification and Characterization of a Novel Chromosomal Aminoglycoside 2′-N-Acetyltransferase, AAC(2′)-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33 |
| title_sort |
identification and characterization of a novel chromosomal aminoglycoside 2′-n-acetyltransferase, aac(2′)-if, from an isolate of a novel providencia species, providencia wenzhouensis r33 |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/1ae5e94ac1d546a4970a7490e8e2c4a1 |
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