Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>

Fungi are the most common pathogens of insects and thus important regulators of their populations. Lipid-binding aegerolysin proteins, which are commonly found in the fungal kingdom, may be involved in several biologically relevant processes including attack and defense against other organisms. Aege...

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Autores principales:	Nada Kraševec, Anastasija Panevska, Špela Lemež, Jaka Razinger, Kristina Sepčić, Gregor Anderluh, Marjetka Podobnik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	beauveriolysin membrane-attack-complex/perforin (MACPF) protein pore-forming proteins entomopathogenic and antagonistic fungi bioinformatics ceramide phosphoethanolamine (CPE) Medicine R
Acceso en línea:	https://doaj.org/article/1ae6b5efb54d4245beca07ca49e1ba6a
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spelling	oai:doaj.org-article:1ae6b5efb54d4245beca07ca49e1ba6a2021-11-25T19:09:07ZLipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>10.3390/toxins131108202072-6651https://doaj.org/article/1ae6b5efb54d4245beca07ca49e1ba6a2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/820https://doaj.org/toc/2072-6651Fungi are the most common pathogens of insects and thus important regulators of their populations. Lipid-binding aegerolysin proteins, which are commonly found in the fungal kingdom, may be involved in several biologically relevant processes including attack and defense against other organisms. Aegerolysins act alone or together with membrane-attack-complex/perforin (MACPF)-like proteins to form transmembrane pores that lead to cell lysis. We performed an in-depth bioinformatics analysis of aegerolysins in entomopathogenic fungi and selected a candidate aegerolysin, beauveriolysin A (BlyA) from <i>Beauveria bassiana</i>. BlyA was expressed as a recombinant protein in <i>Escherichia coli</i>, and purified to further determine its functional and structural properties, including lipid-binding ability. Aegerolysins were found to be encoded in genomes of entomopathogenic fungi, such as <i>Beauveria</i>, <i>Cordyceps</i>, <i>Metarhizium</i> and <i>Ophiocordyceps</i>. Detailed bioinformatics analysis revealed that they are linked to MACPF-like genes in most genomes. We also show that BlyA interacts with an insect-specific membrane lipid. These results were placed in the context of other fungal and bacterial aegerolysins and their partner proteins. We believe that aegerolysins play a role in promoting the entomopathogenic and antagonistic activity of <i>B. bassiana</i>, which is an active ingredient of bioinsecticides.Nada KraševecAnastasija PanevskaŠpela LemežJaka RazingerKristina SepčićGregor AnderluhMarjetka PodobnikMDPI AGarticlebeauveriolysinmembrane-attack-complex/perforin (MACPF) proteinpore-forming proteinsentomopathogenic and antagonistic fungibioinformaticsceramide phosphoethanolamine (CPE)MedicineRENToxins, Vol 13, Iss 820, p 820 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	beauveriolysin membrane-attack-complex/perforin (MACPF) protein pore-forming proteins entomopathogenic and antagonistic fungi bioinformatics ceramide phosphoethanolamine (CPE) Medicine R
spellingShingle	beauveriolysin membrane-attack-complex/perforin (MACPF) protein pore-forming proteins entomopathogenic and antagonistic fungi bioinformatics ceramide phosphoethanolamine (CPE) Medicine R Nada Kraševec Anastasija Panevska Špela Lemež Jaka Razinger Kristina Sepčić Gregor Anderluh Marjetka Podobnik Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
description	Fungi are the most common pathogens of insects and thus important regulators of their populations. Lipid-binding aegerolysin proteins, which are commonly found in the fungal kingdom, may be involved in several biologically relevant processes including attack and defense against other organisms. Aegerolysins act alone or together with membrane-attack-complex/perforin (MACPF)-like proteins to form transmembrane pores that lead to cell lysis. We performed an in-depth bioinformatics analysis of aegerolysins in entomopathogenic fungi and selected a candidate aegerolysin, beauveriolysin A (BlyA) from <i>Beauveria bassiana</i>. BlyA was expressed as a recombinant protein in <i>Escherichia coli</i>, and purified to further determine its functional and structural properties, including lipid-binding ability. Aegerolysins were found to be encoded in genomes of entomopathogenic fungi, such as <i>Beauveria</i>, <i>Cordyceps</i>, <i>Metarhizium</i> and <i>Ophiocordyceps</i>. Detailed bioinformatics analysis revealed that they are linked to MACPF-like genes in most genomes. We also show that BlyA interacts with an insect-specific membrane lipid. These results were placed in the context of other fungal and bacterial aegerolysins and their partner proteins. We believe that aegerolysins play a role in promoting the entomopathogenic and antagonistic activity of <i>B. bassiana</i>, which is an active ingredient of bioinsecticides.
format	article
author	Nada Kraševec Anastasija Panevska Špela Lemež Jaka Razinger Kristina Sepčić Gregor Anderluh Marjetka Podobnik
author_facet	Nada Kraševec Anastasija Panevska Špela Lemež Jaka Razinger Kristina Sepčić Gregor Anderluh Marjetka Podobnik
author_sort	Nada Kraševec
title	Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
title_short	Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
title_full	Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
title_fullStr	Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
title_full_unstemmed	Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>
title_sort	lipid-binding aegerolysin from biocontrol fungus <i>beauveria bassiana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1ae6b5efb54d4245beca07ca49e1ba6a
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Lipid-Binding Aegerolysin from Biocontrol Fungus <i>Beauveria bassiana</i>

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