Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain

Abstract Measurements of water diffusion with MRI have been used as a biomarker of tissue microstructure and heterogeneity. In this study, diffusion kurtosis tensor imaging (DKTI) of the brain was undertaken in 10 healthy volunteers at a clinical field strength of 3 T. Diffusion and kurtosis metrics...

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Autores principales:	Ahmed Maiter, Frank Riemer, Kieren Allinson, Fulvio Zaccagna, Mireia Crispin-Ortuzar, Marcel Gehrung, Mary A. McLean, Andrew N. Priest, James Grist, Tomasz Matys, Martin J. Graves, Ferdia A. Gallagher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ae722fa93164b938aa162886df9eaf8
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spelling	oai:doaj.org-article:1ae722fa93164b938aa162886df9eaf82021-12-02T16:45:47ZInvestigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain10.1038/s41598-021-87857-w2045-2322https://doaj.org/article/1ae722fa93164b938aa162886df9eaf82021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87857-whttps://doaj.org/toc/2045-2322Abstract Measurements of water diffusion with MRI have been used as a biomarker of tissue microstructure and heterogeneity. In this study, diffusion kurtosis tensor imaging (DKTI) of the brain was undertaken in 10 healthy volunteers at a clinical field strength of 3 T. Diffusion and kurtosis metrics were measured in regions-of-interest on the resulting maps and compared with quantitative analysis of normal post-mortem tissue histology from separate age-matched donors. White matter regions showed low diffusion (0.60 ± 0.04 × 10–3 mm2/s) and high kurtosis (1.17 ± 0.06), consistent with a structured heterogeneous environment comprising parallel neuronal fibres. Grey matter showed intermediate diffusion (0.80 ± 0.02 × 10–3 mm2/s) and kurtosis (0.82 ± 0.05) values. An important finding is that the subcortical regions investigated (thalamus, caudate and putamen) showed similar diffusion and kurtosis properties to white matter. Histological staining of the subcortical nuclei demonstrated that the predominant grey matter was permeated by small white matter bundles, which could account for the similar kurtosis to white matter. Quantitative histological analysis demonstrated higher mean tissue kurtosis and vector standard deviation values for white matter (1.08 and 0.81) compared to the subcortical regions (0.34 and 0.59). Mean diffusion on DKTI was positively correlated with tissue kurtosis (r = 0.82, p < 0.05) and negatively correlated with vector standard deviation (r = -0.69, p < 0.05). This study demonstrates how DKTI can be used to study regional structural variations in the cerebral tissue microenvironment and could be used to probe microstructural changes within diseased tissue in the future.Ahmed MaiterFrank RiemerKieren AllinsonFulvio ZaccagnaMireia Crispin-OrtuzarMarcel GehrungMary A. McLeanAndrew N. PriestJames GristTomasz MatysMartin J. GravesFerdia A. GallagherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmed Maiter Frank Riemer Kieren Allinson Fulvio Zaccagna Mireia Crispin-Ortuzar Marcel Gehrung Mary A. McLean Andrew N. Priest James Grist Tomasz Matys Martin J. Graves Ferdia A. Gallagher Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
description	Abstract Measurements of water diffusion with MRI have been used as a biomarker of tissue microstructure and heterogeneity. In this study, diffusion kurtosis tensor imaging (DKTI) of the brain was undertaken in 10 healthy volunteers at a clinical field strength of 3 T. Diffusion and kurtosis metrics were measured in regions-of-interest on the resulting maps and compared with quantitative analysis of normal post-mortem tissue histology from separate age-matched donors. White matter regions showed low diffusion (0.60 ± 0.04 × 10–3 mm2/s) and high kurtosis (1.17 ± 0.06), consistent with a structured heterogeneous environment comprising parallel neuronal fibres. Grey matter showed intermediate diffusion (0.80 ± 0.02 × 10–3 mm2/s) and kurtosis (0.82 ± 0.05) values. An important finding is that the subcortical regions investigated (thalamus, caudate and putamen) showed similar diffusion and kurtosis properties to white matter. Histological staining of the subcortical nuclei demonstrated that the predominant grey matter was permeated by small white matter bundles, which could account for the similar kurtosis to white matter. Quantitative histological analysis demonstrated higher mean tissue kurtosis and vector standard deviation values for white matter (1.08 and 0.81) compared to the subcortical regions (0.34 and 0.59). Mean diffusion on DKTI was positively correlated with tissue kurtosis (r = 0.82, p < 0.05) and negatively correlated with vector standard deviation (r = -0.69, p < 0.05). This study demonstrates how DKTI can be used to study regional structural variations in the cerebral tissue microenvironment and could be used to probe microstructural changes within diseased tissue in the future.
format	article
author	Ahmed Maiter Frank Riemer Kieren Allinson Fulvio Zaccagna Mireia Crispin-Ortuzar Marcel Gehrung Mary A. McLean Andrew N. Priest James Grist Tomasz Matys Martin J. Graves Ferdia A. Gallagher
author_facet	Ahmed Maiter Frank Riemer Kieren Allinson Fulvio Zaccagna Mireia Crispin-Ortuzar Marcel Gehrung Mary A. McLean Andrew N. Priest James Grist Tomasz Matys Martin J. Graves Ferdia A. Gallagher
author_sort	Ahmed Maiter
title	Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
title_short	Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
title_full	Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
title_fullStr	Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
title_full_unstemmed	Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain
title_sort	investigating the relationship between diffusion kurtosis tensor imaging (dkti) and histology within the normal human brain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ae722fa93164b938aa162886df9eaf8
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Investigating the relationship between diffusion kurtosis tensor imaging (DKTI) and histology within the normal human brain

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