Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.

<h4>Background</h4>The desert locust (Schistocerca gregaria) displays a fascinating type of phenotypic plasticity, designated as 'phase polyphenism'. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious...

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Autores principales:	Liesbeth Badisco, Jurgen Huybrechts, Gert Simonet, Heleen Verlinden, Elisabeth Marchal, Roger Huybrechts, Liliane Schoofs, Arnold De Loof, Jozef Vanden Broeck
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ae92b0f487045cb93a32e9602a7ddb8
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spelling	oai:doaj.org-article:1ae92b0f487045cb93a32e9602a7ddb82021-11-18T06:57:04ZTranscriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.1932-620310.1371/journal.pone.0017274https://doaj.org/article/1ae92b0f487045cb93a32e9602a7ddb82011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21445293/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The desert locust (Schistocerca gregaria) displays a fascinating type of phenotypic plasticity, designated as 'phase polyphenism'. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious and gregarious (swarming) phase. Although many of the underlying molecular events remain elusive, the central nervous system (CNS) is expected to play a crucial role in the phase transition process. Locusts have also proven to be interesting model organisms in a physiological and neurobiological research context. However, molecular studies in locusts are hampered by the fact that genome/transcriptome sequence information available for this branch of insects is still limited.<h4>Methodology</h4>We have generated 34,672 raw expressed sequence tags (EST) from the CNS of desert locusts in both phases. These ESTs were assembled in 12,709 unique transcript sequences and nearly 4,000 sequences were functionally annotated. Moreover, the obtained S. gregaria EST information is highly complementary to the existing orthopteran transcriptomic data. Since many novel transcripts encode neuronal signaling and signal transduction components, this paper includes an overview of these sequences. Furthermore, several transcripts being differentially represented in solitarious and gregarious locusts were retrieved from this EST database. The findings highlight the involvement of the CNS in the phase transition process and indicate that this novel annotated database may also add to the emerging knowledge of concomitant neuronal signaling and neuroplasticity events.<h4>Conclusions</h4>In summary, we met the need for novel sequence data from desert locust CNS. To our knowledge, we hereby also present the first insect EST database that is derived from the complete CNS. The obtained S. gregaria EST data constitute an important new source of information that will be instrumental in further unraveling the molecular principles of phase polyphenism, in further establishing locusts as valuable research model organisms and in molecular evolutionary and comparative entomology.Liesbeth BadiscoJurgen HuybrechtsGert SimonetHeleen VerlindenElisabeth MarchalRoger HuybrechtsLiliane SchoofsArnold De LoofJozef Vanden BroeckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17274 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liesbeth Badisco Jurgen Huybrechts Gert Simonet Heleen Verlinden Elisabeth Marchal Roger Huybrechts Liliane Schoofs Arnold De Loof Jozef Vanden Broeck Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
description	<h4>Background</h4>The desert locust (Schistocerca gregaria) displays a fascinating type of phenotypic plasticity, designated as 'phase polyphenism'. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious and gregarious (swarming) phase. Although many of the underlying molecular events remain elusive, the central nervous system (CNS) is expected to play a crucial role in the phase transition process. Locusts have also proven to be interesting model organisms in a physiological and neurobiological research context. However, molecular studies in locusts are hampered by the fact that genome/transcriptome sequence information available for this branch of insects is still limited.<h4>Methodology</h4>We have generated 34,672 raw expressed sequence tags (EST) from the CNS of desert locusts in both phases. These ESTs were assembled in 12,709 unique transcript sequences and nearly 4,000 sequences were functionally annotated. Moreover, the obtained S. gregaria EST information is highly complementary to the existing orthopteran transcriptomic data. Since many novel transcripts encode neuronal signaling and signal transduction components, this paper includes an overview of these sequences. Furthermore, several transcripts being differentially represented in solitarious and gregarious locusts were retrieved from this EST database. The findings highlight the involvement of the CNS in the phase transition process and indicate that this novel annotated database may also add to the emerging knowledge of concomitant neuronal signaling and neuroplasticity events.<h4>Conclusions</h4>In summary, we met the need for novel sequence data from desert locust CNS. To our knowledge, we hereby also present the first insect EST database that is derived from the complete CNS. The obtained S. gregaria EST data constitute an important new source of information that will be instrumental in further unraveling the molecular principles of phase polyphenism, in further establishing locusts as valuable research model organisms and in molecular evolutionary and comparative entomology.
format	article
author	Liesbeth Badisco Jurgen Huybrechts Gert Simonet Heleen Verlinden Elisabeth Marchal Roger Huybrechts Liliane Schoofs Arnold De Loof Jozef Vanden Broeck
author_facet	Liesbeth Badisco Jurgen Huybrechts Gert Simonet Heleen Verlinden Elisabeth Marchal Roger Huybrechts Liliane Schoofs Arnold De Loof Jozef Vanden Broeck
author_sort	Liesbeth Badisco
title	Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
title_short	Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
title_full	Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
title_fullStr	Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
title_full_unstemmed	Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.
title_sort	transcriptome analysis of the desert locust central nervous system: production and annotation of a schistocerca gregaria est database.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1ae92b0f487045cb93a32e9602a7ddb8
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Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.

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