Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death

ABSTRACT Escherichia coli mazEF is a toxin-antitoxin stress-induced module mediating cell death. It requires the quorum-sensing signal (QS) “extracellular death factor” (EDF), the penta-peptide NNWNN (EcEDF), enhancing the endoribonucleolytic activity of E. coli toxin MazF. Here we discovered that E...

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Autores principales:	Sathish Kumar, Ilana Kolodkin-Gal, Hanna Engelberg-Kulka
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Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1af11fa90d414506b3fc191450566a47
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spelling	oai:doaj.org-article:1af11fa90d414506b3fc191450566a472021-11-15T15:40:05ZNovel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death10.1128/mBio.00314-132150-7511https://doaj.org/article/1af11fa90d414506b3fc191450566a472013-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00314-13https://doaj.org/toc/2150-7511ABSTRACT Escherichia coli mazEF is a toxin-antitoxin stress-induced module mediating cell death. It requires the quorum-sensing signal (QS) “extracellular death factor” (EDF), the penta-peptide NNWNN (EcEDF), enhancing the endoribonucleolytic activity of E. coli toxin MazF. Here we discovered that E. coli mazEF-mediated cell death could be triggered by QS peptides from the supernatants (SN) of the Gram-positive bacterium Bacillus subtilis and the Gram-negative bacterium Pseudomonas aeruginosa. In the SN of B. subtilis, we found one EDF, the hexapeptide RGQQNE, called BsEDF. In the SN of P. aeruginosa, we found three EDFs: the nonapeptide INEQTVVTK, called PaEDF-1, and two hexadecapeptides, VEVSDDGSGGNTSLSQ, called PaEDF-2, and APKLSDGAAAGYVTKA, called PaEDF-3. When added to a diluted E. coli cultures, each of these peptides acted as an interspecies EDF that triggered mazEF-mediated death. Furthermore, though their sequences are very different, each of these EDFs amplified the endoribonucleolytic activity of E. coli MazF, probably by interacting with different sites on E. coli MazF. Finally, we suggest that EDFs may become the basis for a new class of antibiotics that trigger death from outside the bacterial cells. IMPORTANCE Bacteria communicate with one another via quorum-sensing signal (QS) molecules. QS provides a mechanism for bacteria to monitor each other’s presence and to modulate gene expression in response to population density. Previously, we added E. coli EDF (EcEDF), the peptide NNWNN, to this list of QS molecules. Here we extended the group of QS peptides to several additional different peptides. The new EDFs are produced by two other bacteria, Bacillus subtilis and Pseudomonas aeruginosa. Thus, in this study we established a “new family of EDFs.” This family provides the first example of quorum-sensing molecules participating in interspecies bacterial cell death. Furthermore, each of these peptides provides the basis of a new class of antibiotics triggering death by acting from outside the cell.Sathish KumarIlana Kolodkin-GalHanna Engelberg-KulkaAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 3 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sathish Kumar Ilana Kolodkin-Gal Hanna Engelberg-Kulka Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
description	ABSTRACT Escherichia coli mazEF is a toxin-antitoxin stress-induced module mediating cell death. It requires the quorum-sensing signal (QS) “extracellular death factor” (EDF), the penta-peptide NNWNN (EcEDF), enhancing the endoribonucleolytic activity of E. coli toxin MazF. Here we discovered that E. coli mazEF-mediated cell death could be triggered by QS peptides from the supernatants (SN) of the Gram-positive bacterium Bacillus subtilis and the Gram-negative bacterium Pseudomonas aeruginosa. In the SN of B. subtilis, we found one EDF, the hexapeptide RGQQNE, called BsEDF. In the SN of P. aeruginosa, we found three EDFs: the nonapeptide INEQTVVTK, called PaEDF-1, and two hexadecapeptides, VEVSDDGSGGNTSLSQ, called PaEDF-2, and APKLSDGAAAGYVTKA, called PaEDF-3. When added to a diluted E. coli cultures, each of these peptides acted as an interspecies EDF that triggered mazEF-mediated death. Furthermore, though their sequences are very different, each of these EDFs amplified the endoribonucleolytic activity of E. coli MazF, probably by interacting with different sites on E. coli MazF. Finally, we suggest that EDFs may become the basis for a new class of antibiotics that trigger death from outside the bacterial cells. IMPORTANCE Bacteria communicate with one another via quorum-sensing signal (QS) molecules. QS provides a mechanism for bacteria to monitor each other’s presence and to modulate gene expression in response to population density. Previously, we added E. coli EDF (EcEDF), the peptide NNWNN, to this list of QS molecules. Here we extended the group of QS peptides to several additional different peptides. The new EDFs are produced by two other bacteria, Bacillus subtilis and Pseudomonas aeruginosa. Thus, in this study we established a “new family of EDFs.” This family provides the first example of quorum-sensing molecules participating in interspecies bacterial cell death. Furthermore, each of these peptides provides the basis of a new class of antibiotics triggering death by acting from outside the cell.
format	article
author	Sathish Kumar Ilana Kolodkin-Gal Hanna Engelberg-Kulka
author_facet	Sathish Kumar Ilana Kolodkin-Gal Hanna Engelberg-Kulka
author_sort	Sathish Kumar
title	Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
title_short	Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
title_full	Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
title_fullStr	Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
title_full_unstemmed	Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death
title_sort	novel quorum-sensing peptides mediating interspecies bacterial cell death
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/1af11fa90d414506b3fc191450566a47
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Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death

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