Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.

Muscle precursor cells known as myoblasts are essential for muscle development and regeneration. Notch signaling is an ancient intercellular communication mechanism that plays prominent roles in controlling the myogenic program of myoblasts. Currently whether and how the myogenic cues feedback to re...

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Autores principales:	Haifeng Zhang, Renjie Shang, Pengpeng Bi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1afa77cfdce446979f30150a4bc33693
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spelling	oai:doaj.org-article:1afa77cfdce446979f30150a4bc336932021-12-02T20:02:53ZFeedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.1553-73901553-740410.1371/journal.pgen.1009729https://doaj.org/article/1afa77cfdce446979f30150a4bc336932021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009729https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Muscle precursor cells known as myoblasts are essential for muscle development and regeneration. Notch signaling is an ancient intercellular communication mechanism that plays prominent roles in controlling the myogenic program of myoblasts. Currently whether and how the myogenic cues feedback to refine Notch activities in these cells are largely unknown. Here, by mouse and human gene gain/loss-of-function studies, we report that MyoD directly turns on the expression of Notch-ligand gene Dll1 which activates Notch pathway to prevent precautious differentiation in neighboring myoblasts, while autonomously inhibits Notch to facilitate a myogenic program in Dll1 expressing cells. Mechanistically, we studied cis-regulatory DNA motifs underlying the MyoD-Dll1-Notch axis in vivo by characterizing myogenesis of a novel E-box deficient mouse model, as well as in human cells through CRISPR-mediated interference. These results uncovered the crucial transcriptional mechanism that mediates the reciprocal controls of Notch and myogenesis.Haifeng ZhangRenjie ShangPengpeng BiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009729 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Haifeng Zhang Renjie Shang Pengpeng Bi Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
description	Muscle precursor cells known as myoblasts are essential for muscle development and regeneration. Notch signaling is an ancient intercellular communication mechanism that plays prominent roles in controlling the myogenic program of myoblasts. Currently whether and how the myogenic cues feedback to refine Notch activities in these cells are largely unknown. Here, by mouse and human gene gain/loss-of-function studies, we report that MyoD directly turns on the expression of Notch-ligand gene Dll1 which activates Notch pathway to prevent precautious differentiation in neighboring myoblasts, while autonomously inhibits Notch to facilitate a myogenic program in Dll1 expressing cells. Mechanistically, we studied cis-regulatory DNA motifs underlying the MyoD-Dll1-Notch axis in vivo by characterizing myogenesis of a novel E-box deficient mouse model, as well as in human cells through CRISPR-mediated interference. These results uncovered the crucial transcriptional mechanism that mediates the reciprocal controls of Notch and myogenesis.
format	article
author	Haifeng Zhang Renjie Shang Pengpeng Bi
author_facet	Haifeng Zhang Renjie Shang Pengpeng Bi
author_sort	Haifeng Zhang
title	Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
title_short	Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
title_full	Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
title_fullStr	Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
title_full_unstemmed	Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.
title_sort	feedback regulation of notch signaling and myogenesis connected by myod-dll1 axis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1afa77cfdce446979f30150a4bc33693
work_keys_str_mv	AT haifengzhang feedbackregulationofnotchsignalingandmyogenesisconnectedbymyoddll1axis AT renjieshang feedbackregulationofnotchsignalingandmyogenesisconnectedbymyoddll1axis AT pengpengbi feedbackregulationofnotchsignalingandmyogenesisconnectedbymyoddll1axis
_version_	1718375645998743552

Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.

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