Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

The performance of a mixed microbial community was tested in lab-scale power-to-methane reactors at 55 °C. The main aim was to uncover the responses of the community to starvation and stoichiometric H<sub>2</sub>/CO<sub>2</sub> supply as the sole substrate. Fed-batch reactors...

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Autores principales:	Márk Szuhaj, Roland Wirth, Zoltán Bagi, Gergely Maróti, Gábor Rákhely, Kornél L. Kovács
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	power-to-gas thermophilic biogas fed-batch reactor <i>Methanothermobacter</i> metagenome starvation Technology T
Acceso en línea:	https://doaj.org/article/1afbdd60633c44399af9ecd401e0544b
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spelling	oai:doaj.org-article:1afbdd60633c44399af9ecd401e0544b2021-11-11T16:03:38ZDevelopment of Stable Mixed Microbiota for High Yield Power to Methane Conversion10.3390/en142173361996-1073https://doaj.org/article/1afbdd60633c44399af9ecd401e0544b2021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1073/14/21/7336https://doaj.org/toc/1996-1073The performance of a mixed microbial community was tested in lab-scale power-to-methane reactors at 55 °C. The main aim was to uncover the responses of the community to starvation and stoichiometric H<sub>2</sub>/CO<sub>2</sub> supply as the sole substrate. Fed-batch reactors were inoculated with the fermentation effluent of a thermophilic biogas plant. Various volumes of pure H<sub>2</sub>/CO<sub>2</sub> gas mixtures were injected into the headspace daily and the process parameters were followed. Gas volumes and composition were measured by gas-chromatography, the headspace was replaced with N<sub>2</sub> prior to the daily H<sub>2</sub>/CO<sub>2</sub> injection. Total DNA samples, collected at the beginning and end (day 71), were analyzed by metagenome sequencing. Low levels of H<sub>2</sub> triggered immediate CH<sub>4</sub> evolution utilizing CO<sub>2</sub>/HCO<sub>3</sub><sup>−</sup> dissolved in the fermentation effluent. Biomethanation continued when H<sub>2</sub>/CO<sub>2</sub> was supplied. On the contrary, biomethane formation was inhibited at higher initial H<sub>2</sub> doses and concomitant acetate formation indicated homoacetogenesis. Biomethane production started upon daily delivery of stoichiometric H<sub>2</sub>/CO<sub>2</sub>. The fed-batch operational mode allowed high H<sub>2</sub> injection and consumption rates albeit intermittent operation conditions. Methane was enriched up to 95% CH<sub>4</sub> content and the H<sub>2</sub> consumption rate attained a remarkable 1000 mL·L<sup>−1</sup>·d<sup>−1</sup>. The microbial community spontaneously selected the genus <i>Methanothermobacter</i> in the enriched cultures.Márk SzuhajRoland WirthZoltán BagiGergely MarótiGábor RákhelyKornél L. KovácsMDPI AGarticlepower-to-gasthermophilic biogasfed-batch reactor<i>Methanothermobacter</i>metagenomestarvationTechnologyTENEnergies, Vol 14, Iss 7336, p 7336 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	power-to-gas thermophilic biogas fed-batch reactor <i>Methanothermobacter</i> metagenome starvation Technology T
spellingShingle	power-to-gas thermophilic biogas fed-batch reactor <i>Methanothermobacter</i> metagenome starvation Technology T Márk Szuhaj Roland Wirth Zoltán Bagi Gergely Maróti Gábor Rákhely Kornél L. Kovács Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
description	The performance of a mixed microbial community was tested in lab-scale power-to-methane reactors at 55 °C. The main aim was to uncover the responses of the community to starvation and stoichiometric H<sub>2</sub>/CO<sub>2</sub> supply as the sole substrate. Fed-batch reactors were inoculated with the fermentation effluent of a thermophilic biogas plant. Various volumes of pure H<sub>2</sub>/CO<sub>2</sub> gas mixtures were injected into the headspace daily and the process parameters were followed. Gas volumes and composition were measured by gas-chromatography, the headspace was replaced with N<sub>2</sub> prior to the daily H<sub>2</sub>/CO<sub>2</sub> injection. Total DNA samples, collected at the beginning and end (day 71), were analyzed by metagenome sequencing. Low levels of H<sub>2</sub> triggered immediate CH<sub>4</sub> evolution utilizing CO<sub>2</sub>/HCO<sub>3</sub><sup>−</sup> dissolved in the fermentation effluent. Biomethanation continued when H<sub>2</sub>/CO<sub>2</sub> was supplied. On the contrary, biomethane formation was inhibited at higher initial H<sub>2</sub> doses and concomitant acetate formation indicated homoacetogenesis. Biomethane production started upon daily delivery of stoichiometric H<sub>2</sub>/CO<sub>2</sub>. The fed-batch operational mode allowed high H<sub>2</sub> injection and consumption rates albeit intermittent operation conditions. Methane was enriched up to 95% CH<sub>4</sub> content and the H<sub>2</sub> consumption rate attained a remarkable 1000 mL·L<sup>−1</sup>·d<sup>−1</sup>. The microbial community spontaneously selected the genus <i>Methanothermobacter</i> in the enriched cultures.
format	article
author	Márk Szuhaj Roland Wirth Zoltán Bagi Gergely Maróti Gábor Rákhely Kornél L. Kovács
author_facet	Márk Szuhaj Roland Wirth Zoltán Bagi Gergely Maróti Gábor Rákhely Kornél L. Kovács
author_sort	Márk Szuhaj
title	Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
title_short	Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
title_full	Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
title_fullStr	Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
title_full_unstemmed	Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion
title_sort	development of stable mixed microbiota for high yield power to methane conversion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1afbdd60633c44399af9ecd401e0544b
work_keys_str_mv	AT markszuhaj developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion AT rolandwirth developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion AT zoltanbagi developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion AT gergelymaroti developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion AT gaborrakhely developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion AT kornellkovacs developmentofstablemixedmicrobiotaforhighyieldpowertomethaneconversion
_version_	1718432401562009600

Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

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