Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film

PVA (poly (vinyl alcohol)) hydrogel is one of the anticipated materials for artificial cartilage. In previous research, authors found that the structure of protein boundary film on rubbing surface is a key essence for wear reduction of PVA hydrogel. The aim of this study is to understand the mechani...

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Autores principales:	Seido Yarimitsu, Kazuhiro Nakashima, Yoshinori Sawae, Teruo Murakami
Formato:	article
Lenguaje:	EN
Publicado:	Japanese Society of Tribologists 2007
Materias:	artificial cartilage wear reduction protein boundary film in situ observation Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1afe04dd31e640d0adb674af9ee7c219
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spelling	oai:doaj.org-article:1afe04dd31e640d0adb674af9ee7c2192021-11-05T09:29:42ZStudy on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film1881-219810.2474/trol.2.114https://doaj.org/article/1afe04dd31e640d0adb674af9ee7c2192007-12-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/trol/2/4/2_4_114/_pdf/-char/enhttps://doaj.org/toc/1881-2198PVA (poly (vinyl alcohol)) hydrogel is one of the anticipated materials for artificial cartilage. In previous research, authors found that the structure of protein boundary film on rubbing surface is a key essence for wear reduction of PVA hydrogel. The aim of this study is to understand the mechanisms of wear reduction of PVA hydrogel through in situ observation on forming protein boundary film on the rubbing surface. Reciprocating apparatus was constructed on the stage in inverted fluorescent microscope. A sliding pair of a spherical reciprocating upper specimen of PVA hydrogel and a flat stationary lower specimen of cover glass was tested. Lubricants were single and binary protein solutions, in which fluorescent-labeled bovine serum albumin and human serum γ-globulin were used as additives. When single protein was added to lubricants, albumin formed a loosely adsorbed film on rubbing surface, whereas γ-globulin adsorbed firmly and formed a smooth and uniform boundary film. When both of albumin and γ-globulin were added into lubricants, the stability of protein boundary film varied with the concentration and ratio of two kinds of proteins in lubricants. These results indicate that there is suitable content and ratio of proteins added into lubricants to form a stable boundary film.Seido YarimitsuKazuhiro NakashimaYoshinori SawaeTeruo MurakamiJapanese Society of Tribologistsarticleartificial cartilagewear reductionprotein boundary filmin situ observationPhysicsQC1-999Engineering (General). Civil engineering (General)TA1-2040Mechanical engineering and machineryTJ1-1570ChemistryQD1-999ENTribology Online, Vol 2, Iss 4, Pp 114-119 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	artificial cartilage wear reduction protein boundary film in situ observation Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
spellingShingle	artificial cartilage wear reduction protein boundary film in situ observation Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999 Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
description	PVA (poly (vinyl alcohol)) hydrogel is one of the anticipated materials for artificial cartilage. In previous research, authors found that the structure of protein boundary film on rubbing surface is a key essence for wear reduction of PVA hydrogel. The aim of this study is to understand the mechanisms of wear reduction of PVA hydrogel through in situ observation on forming protein boundary film on the rubbing surface. Reciprocating apparatus was constructed on the stage in inverted fluorescent microscope. A sliding pair of a spherical reciprocating upper specimen of PVA hydrogel and a flat stationary lower specimen of cover glass was tested. Lubricants were single and binary protein solutions, in which fluorescent-labeled bovine serum albumin and human serum γ-globulin were used as additives. When single protein was added to lubricants, albumin formed a loosely adsorbed film on rubbing surface, whereas γ-globulin adsorbed firmly and formed a smooth and uniform boundary film. When both of albumin and γ-globulin were added into lubricants, the stability of protein boundary film varied with the concentration and ratio of two kinds of proteins in lubricants. These results indicate that there is suitable content and ratio of proteins added into lubricants to form a stable boundary film.
format	article
author	Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami
author_facet	Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami
author_sort	Seido Yarimitsu
title	Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
title_short	Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
title_full	Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
title_fullStr	Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
title_full_unstemmed	Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film
title_sort	study on the mechanisms of wear reduction of artificial cartilage through in situ observation on forming protein boundary film
publisher	Japanese Society of Tribologists
publishDate	2007
url	https://doaj.org/article/1afe04dd31e640d0adb674af9ee7c219
work_keys_str_mv	AT seidoyarimitsu studyonthemechanismsofwearreductionofartificialcartilagethroughinsituobservationonformingproteinboundaryfilm AT kazuhironakashima studyonthemechanismsofwearreductionofartificialcartilagethroughinsituobservationonformingproteinboundaryfilm AT yoshinorisawae studyonthemechanismsofwearreductionofartificialcartilagethroughinsituobservationonformingproteinboundaryfilm AT teruomurakami studyonthemechanismsofwearreductionofartificialcartilagethroughinsituobservationonformingproteinboundaryfilm
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Study on the Mechanisms of Wear Reduction of Artificial Cartilage through in Situ Observation on Forming Protein Boundary Film

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