ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM

BACKGROUND & OBJECTIVE: Hypericum perforatum is a medicinal plant which has been known in traditional medicine as anti-inflammatory and healing agent. This study was done to determine the effect of anti inflammatory, analgesic activity and acute toxicity of this plant.METHODS: In this experiment...

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Autores principales:	M M. Mahmoudi, A Javanmardi, K Morteza Semnani, M Saeedi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2006
Materias:	hypericum perforatum anti-inflammatory analgesic acute toxicity hypericin Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1b041de0c9034f3c9d3e24cf173b9835
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spelling	oai:doaj.org-article:1b041de0c9034f3c9d3e24cf173b98352021-11-10T09:12:57ZANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM1561-41072251-7170https://doaj.org/article/1b041de0c9034f3c9d3e24cf173b98352006-08-01T00:00:00Zhttp://jbums.org/article-1-3007-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND & OBJECTIVE: Hypericum perforatum is a medicinal plant which has been known in traditional medicine as anti-inflammatory and healing agent. This study was done to determine the effect of anti inflammatory, analgesic activity and acute toxicity of this plant.METHODS: In this experimental study, the plant extracted by soxhlet with methanol. The anti-inflammatory and analgesic effects of extract were studied using carrageenan induced rat paw edema, formalin, hot plate and writhing tests. Motor coordination was assessed using Rotarod test and acute toxicity (LD50) was determined using probit method. ANOVA followed by Student-Newman-Keuls test were used to determine significant differences between groups and p<0.05 was considered significant. FINDINGS: Similar activity against carrageenan-induced rat paw edema was observed with hypericum perforatum extract (100 and 150 mg/kg) and indomethacin (4 mg/kg). In the formalin test, the extract (25-250 mg/kg, i.p.) caused graded inhibition of both phases of formalin-induced pain (p<0.001). In the hot plate test, the i.p. administration of the extract at the doses of 25- 250 mg/kg significantly raised the pain threshold at a observation time of 30 min in comparison with the control group (p<0.001). In the writhing test, the extract at doses of 25 mg/kg (p<0.05), 50, 75, 100 and 150mg/kg (p<0.001) produced a significant decrease in the number of writhing in comparison with the control group. The extract, at antinociceptive doses, did not affect motor coordination of animals when assessed in the rotarod model. The 72 hr acute LD50 value of this extract after i.p. administration in mice was 1111.47 mg/kg. The total content of hypericins was determined 0.101±0.003 in this plant. CONCLUSION: Based on these results, hypericum perforatum can be introduced as an anti-analgesic and anti-inflammatory plant.M M. Mahmoudi,A JavanmardiK Morteza SemnaniM SaeediBabol University of Medical Sciencesarticlehypericum perforatumanti-inflammatoryanalgesicacute toxicityhypericinMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 8, Iss 4, Pp 7-14 (2006)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	hypericum perforatum anti-inflammatory analgesic acute toxicity hypericin Medicine R Medicine (General) R5-920
spellingShingle	hypericum perforatum anti-inflammatory analgesic acute toxicity hypericin Medicine R Medicine (General) R5-920 M M. Mahmoudi, A Javanmardi K Morteza Semnani M Saeedi ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
description	BACKGROUND & OBJECTIVE: Hypericum perforatum is a medicinal plant which has been known in traditional medicine as anti-inflammatory and healing agent. This study was done to determine the effect of anti inflammatory, analgesic activity and acute toxicity of this plant.METHODS: In this experimental study, the plant extracted by soxhlet with methanol. The anti-inflammatory and analgesic effects of extract were studied using carrageenan induced rat paw edema, formalin, hot plate and writhing tests. Motor coordination was assessed using Rotarod test and acute toxicity (LD50) was determined using probit method. ANOVA followed by Student-Newman-Keuls test were used to determine significant differences between groups and p<0.05 was considered significant. FINDINGS: Similar activity against carrageenan-induced rat paw edema was observed with hypericum perforatum extract (100 and 150 mg/kg) and indomethacin (4 mg/kg). In the formalin test, the extract (25-250 mg/kg, i.p.) caused graded inhibition of both phases of formalin-induced pain (p<0.001). In the hot plate test, the i.p. administration of the extract at the doses of 25- 250 mg/kg significantly raised the pain threshold at a observation time of 30 min in comparison with the control group (p<0.001). In the writhing test, the extract at doses of 25 mg/kg (p<0.05), 50, 75, 100 and 150mg/kg (p<0.001) produced a significant decrease in the number of writhing in comparison with the control group. The extract, at antinociceptive doses, did not affect motor coordination of animals when assessed in the rotarod model. The 72 hr acute LD50 value of this extract after i.p. administration in mice was 1111.47 mg/kg. The total content of hypericins was determined 0.101±0.003 in this plant. CONCLUSION: Based on these results, hypericum perforatum can be introduced as an anti-analgesic and anti-inflammatory plant.
format	article
author	M M. Mahmoudi, A Javanmardi K Morteza Semnani M Saeedi
author_facet	M M. Mahmoudi, A Javanmardi K Morteza Semnani M Saeedi
author_sort	M M. Mahmoudi,
title	ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
title_short	ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
title_full	ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
title_fullStr	ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
title_full_unstemmed	ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM
title_sort	anti-inflammatory, analgesic activity, acute toxicity and hypericins content of iranian hypericum perforatum
publisher	Babol University of Medical Sciences
publishDate	2006
url	https://doaj.org/article/1b041de0c9034f3c9d3e24cf173b9835
work_keys_str_mv	AT mmmahmoudi antiinflammatoryanalgesicactivityacutetoxicityandhypericinscontentofiranianhypericumperforatum AT ajavanmardi antiinflammatoryanalgesicactivityacutetoxicityandhypericinscontentofiranianhypericumperforatum AT kmortezasemnani antiinflammatoryanalgesicactivityacutetoxicityandhypericinscontentofiranianhypericumperforatum AT msaeedi antiinflammatoryanalgesicactivityacutetoxicityandhypericinscontentofiranianhypericumperforatum
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ANTI-INFLAMMATORY, ANALGESIC ACTIVITY, ACUTE TOXICITY AND HYPERICINS CONTENT OF IRANIAN HYPERICUM PERFORATUM

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