APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.

APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.

<h4>Background</h4>CD4+ T lymphocytes are the primary targets of HIV1 but cannot be infected when fully quiescent, due to a post-entry block preventing the completion of reverse transcription. Chiu et al. recently proposed that this restriction reflects the action of APOBEC3G (A3G). They...

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Autores principales: Francesca R Santoni de Sio, Didier Trono
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2009
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Acceso en línea:https://doaj.org/article/1b1000cfba5c4167bed75b030dedd744
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spelling oai:doaj.org-article:1b1000cfba5c4167bed75b030dedd7442021-11-25T06:21:07ZAPOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.1932-620310.1371/journal.pone.0006571https://doaj.org/article/1b1000cfba5c4167bed75b030dedd7442009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19668336/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>CD4+ T lymphocytes are the primary targets of HIV1 but cannot be infected when fully quiescent, due to a post-entry block preventing the completion of reverse transcription. Chiu et al. recently proposed that this restriction reflects the action of APOBEC3G (A3G). They further suggested that T cell activation abrogates the A3G-mediated block by directing this protein to a high molecular mass complex.<h4>Methodology/principal findings</h4>In the present work, we sought to explore further this model. However, we found that effective suppression of A3G by combined RNA interference and expression of HIV1 Vif does not relieve the restrictive phenotype of post-activation resting T cells. We also failed to find a correlation between HIV resistance and the presence of A3G in a low molecular complex in primary T cells.<h4>Conclusions/significance</h4>We conclude that A3G is unlikely to play a role in the HIV restrictive phenotype of quiescent T lymphocytes.Francesca R Santoni de SioDidier TronoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 8, p e6571 (2009)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Francesca R Santoni de Sio
Didier Trono
APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
description <h4>Background</h4>CD4+ T lymphocytes are the primary targets of HIV1 but cannot be infected when fully quiescent, due to a post-entry block preventing the completion of reverse transcription. Chiu et al. recently proposed that this restriction reflects the action of APOBEC3G (A3G). They further suggested that T cell activation abrogates the A3G-mediated block by directing this protein to a high molecular mass complex.<h4>Methodology/principal findings</h4>In the present work, we sought to explore further this model. However, we found that effective suppression of A3G by combined RNA interference and expression of HIV1 Vif does not relieve the restrictive phenotype of post-activation resting T cells. We also failed to find a correlation between HIV resistance and the presence of A3G in a low molecular complex in primary T cells.<h4>Conclusions/significance</h4>We conclude that A3G is unlikely to play a role in the HIV restrictive phenotype of quiescent T lymphocytes.
format article
author Francesca R Santoni de Sio
Didier Trono
author_facet Francesca R Santoni de Sio
Didier Trono
author_sort Francesca R Santoni de Sio
title APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
title_short APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
title_full APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
title_fullStr APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
title_full_unstemmed APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection.
title_sort apobec3g-depleted resting cd4+ t cells remain refractory to hiv1 infection.
publisher Public Library of Science (PLoS)
publishDate 2009
url https://doaj.org/article/1b1000cfba5c4167bed75b030dedd744
work_keys_str_mv AT francescarsantonidesio apobec3gdepletedrestingcd4tcellsremainrefractorytohiv1infection
AT didiertrono apobec3gdepletedrestingcd4tcellsremainrefractorytohiv1infection
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