High-throughput mutagenesis using a two-fragment PCR approach

Abstract Site-directed scanning mutagenesis is a powerful protein engineering technique which allows studies of protein functionality at single amino acid resolution and design of stabilized proteins for structural and biophysical work. However, creating libraries of hundreds of mutants remains a ch...

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Autores principales: Franziska M. Heydenreich, Tamara Miljuš, Rolf Jaussi, Roger Benoit, Dalibor Milić, Dmitry B. Veprintsev
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/1b11ce312b1949debc1d11e568ec22af
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