Altered high-density lipoprotein composition and functions during severe COVID-19

Abstract Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL part...

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Autores principales:	Floran Begue, Sébastien Tanaka, Zarouki Mouktadi, Philippe Rondeau, Bryan Veeren, Nicolas Diotel, Alexy Tran-Dinh, Tiphaine Robert, Erick Vélia, Patrick Mavingui, Marie Lagrange-Xélot, Philippe Montravers, David Couret, Olivier Meilhac
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b14a13ba4d34ff68e8ff0d250c12c6e
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spelling	oai:doaj.org-article:1b14a13ba4d34ff68e8ff0d250c12c6e2021-12-02T10:48:31ZAltered high-density lipoprotein composition and functions during severe COVID-1910.1038/s41598-021-81638-12045-2322https://doaj.org/article/1b14a13ba4d34ff68e8ff0d250c12c6e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81638-1https://doaj.org/toc/2045-2322Abstract Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients.Floran BegueSébastien TanakaZarouki MouktadiPhilippe RondeauBryan VeerenNicolas DiotelAlexy Tran-DinhTiphaine RobertErick VéliaPatrick MavinguiMarie Lagrange-XélotPhilippe MontraversDavid CouretOlivier MeilhacNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Floran Begue Sébastien Tanaka Zarouki Mouktadi Philippe Rondeau Bryan Veeren Nicolas Diotel Alexy Tran-Dinh Tiphaine Robert Erick Vélia Patrick Mavingui Marie Lagrange-Xélot Philippe Montravers David Couret Olivier Meilhac Altered high-density lipoprotein composition and functions during severe COVID-19
description	Abstract Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients.
format	article
author	Floran Begue Sébastien Tanaka Zarouki Mouktadi Philippe Rondeau Bryan Veeren Nicolas Diotel Alexy Tran-Dinh Tiphaine Robert Erick Vélia Patrick Mavingui Marie Lagrange-Xélot Philippe Montravers David Couret Olivier Meilhac
author_facet	Floran Begue Sébastien Tanaka Zarouki Mouktadi Philippe Rondeau Bryan Veeren Nicolas Diotel Alexy Tran-Dinh Tiphaine Robert Erick Vélia Patrick Mavingui Marie Lagrange-Xélot Philippe Montravers David Couret Olivier Meilhac
author_sort	Floran Begue
title	Altered high-density lipoprotein composition and functions during severe COVID-19
title_short	Altered high-density lipoprotein composition and functions during severe COVID-19
title_full	Altered high-density lipoprotein composition and functions during severe COVID-19
title_fullStr	Altered high-density lipoprotein composition and functions during severe COVID-19
title_full_unstemmed	Altered high-density lipoprotein composition and functions during severe COVID-19
title_sort	altered high-density lipoprotein composition and functions during severe covid-19
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1b14a13ba4d34ff68e8ff0d250c12c6e
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Altered high-density lipoprotein composition and functions during severe COVID-19

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