Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes

Abstract Background Efficient and topical delivery of drugs is essential for maximized efficacy and minimized toxicity. In this study, we aimed to design an exosome-based drug delivery platform endowed with the ability of escaping from phagocytosis at non-target organs and controllably releasing dru...

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Autores principales: Yitong Guo, Zhuo Wan, Ping Zhao, Mengying Wei, Yunnan Liu, Te Bu, Wenqi Sun, Zhelong Li, Lijun Yuan
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Publicado: BMC 2021
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Acceso en línea:https://doaj.org/article/1b1e9096a06840269603bf229f1531d2
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spelling oai:doaj.org-article:1b1e9096a06840269603bf229f1531d22021-12-05T12:19:08ZUltrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes10.1186/s12951-021-01145-31477-3155https://doaj.org/article/1b1e9096a06840269603bf229f1531d22021-12-01T00:00:00Zhttps://doi.org/10.1186/s12951-021-01145-3https://doaj.org/toc/1477-3155Abstract Background Efficient and topical delivery of drugs is essential for maximized efficacy and minimized toxicity. In this study, we aimed to design an exosome-based drug delivery platform endowed with the ability of escaping from phagocytosis at non-target organs and controllably releasing drugs at targeted location. Results The swtichable stealth coat CP05-TK-mPEG was synthesized and anchored onto exosomes through the interaction between peptide CP05 and exosomal surface marker CD63. Chlorin e6 (Ce6) was loaded into exosomes by direct incubation. Controllable removal of PEG could be achieved by breaking thioketal (TK) through reactive oxygen species (ROS), which was produced by Ce6 under ultrasound irradiation. The whole platform was called SmartExo. The stealth effects were analyzed in RAW264.7 cells and C57BL/6 mice via tracing the exosomes. To confirm the efficacy of the engineered smart exosomes, Bone morphogenetic protein 7 (Bmp7) mRNA was encapsulated into exosomes by transfection of overexpressing plasmid, followed by stealth coating, with the exosomes designated as SmartExo@Bmp7. Therapeutic advantages of SmartExo@Bmp7 were proved by targeted delivering Bmp7 mRNA to omental adipose tissue (OAT) of obese C57BL/6 mice for browning induction. SmartExo platform was successfully constructed without changing the basic characteristics of exosomes. The engineered exosomes effectively escaped from the phagocytosis by RAW264.7 and non-target organs. In addition, the SmartExo could be uptaken locally on-demand by ultrasound mediated removal of the stealth coat. Compared with control exosomes, SmartExo@Bmp7 effectively delivered Bmp7 mRNA into OAT upon ultrasound irradiation, and induced OAT browning, as evidenced by the histology of OAT and increased expression of uncoupling protein 1 (Ucp1). Conclusions The proposed SmartExo-based delivery platform, which minimizes side effects and maximizing drug efficacy, offers a novel safe and efficient approach for targeted drug delivery. As a proof, the SmartExo@Bmp7 induced local white adipose tissue browning, and it would be a promising strategy for anti-obesity therapy. Graphical AbstractYitong GuoZhuo WanPing ZhaoMengying WeiYunnan LiuTe BuWenqi SunZhelong LiLijun YuanBMCarticleTargeted drug deliveryExosomesStealth techniqueUltrasoundBmp7White fat browningBiotechnologyTP248.13-248.65Medical technologyR855-855.5ENJournal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Targeted drug delivery
Exosomes
Stealth technique
Ultrasound
Bmp7
White fat browning
Biotechnology
TP248.13-248.65
Medical technology
R855-855.5
spellingShingle Targeted drug delivery
Exosomes
Stealth technique
Ultrasound
Bmp7
White fat browning
Biotechnology
TP248.13-248.65
Medical technology
R855-855.5
Yitong Guo
Zhuo Wan
Ping Zhao
Mengying Wei
Yunnan Liu
Te Bu
Wenqi Sun
Zhelong Li
Lijun Yuan
Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
description Abstract Background Efficient and topical delivery of drugs is essential for maximized efficacy and minimized toxicity. In this study, we aimed to design an exosome-based drug delivery platform endowed with the ability of escaping from phagocytosis at non-target organs and controllably releasing drugs at targeted location. Results The swtichable stealth coat CP05-TK-mPEG was synthesized and anchored onto exosomes through the interaction between peptide CP05 and exosomal surface marker CD63. Chlorin e6 (Ce6) was loaded into exosomes by direct incubation. Controllable removal of PEG could be achieved by breaking thioketal (TK) through reactive oxygen species (ROS), which was produced by Ce6 under ultrasound irradiation. The whole platform was called SmartExo. The stealth effects were analyzed in RAW264.7 cells and C57BL/6 mice via tracing the exosomes. To confirm the efficacy of the engineered smart exosomes, Bone morphogenetic protein 7 (Bmp7) mRNA was encapsulated into exosomes by transfection of overexpressing plasmid, followed by stealth coating, with the exosomes designated as SmartExo@Bmp7. Therapeutic advantages of SmartExo@Bmp7 were proved by targeted delivering Bmp7 mRNA to omental adipose tissue (OAT) of obese C57BL/6 mice for browning induction. SmartExo platform was successfully constructed without changing the basic characteristics of exosomes. The engineered exosomes effectively escaped from the phagocytosis by RAW264.7 and non-target organs. In addition, the SmartExo could be uptaken locally on-demand by ultrasound mediated removal of the stealth coat. Compared with control exosomes, SmartExo@Bmp7 effectively delivered Bmp7 mRNA into OAT upon ultrasound irradiation, and induced OAT browning, as evidenced by the histology of OAT and increased expression of uncoupling protein 1 (Ucp1). Conclusions The proposed SmartExo-based delivery platform, which minimizes side effects and maximizing drug efficacy, offers a novel safe and efficient approach for targeted drug delivery. As a proof, the SmartExo@Bmp7 induced local white adipose tissue browning, and it would be a promising strategy for anti-obesity therapy. Graphical Abstract
format article
author Yitong Guo
Zhuo Wan
Ping Zhao
Mengying Wei
Yunnan Liu
Te Bu
Wenqi Sun
Zhelong Li
Lijun Yuan
author_facet Yitong Guo
Zhuo Wan
Ping Zhao
Mengying Wei
Yunnan Liu
Te Bu
Wenqi Sun
Zhelong Li
Lijun Yuan
author_sort Yitong Guo
title Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
title_short Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
title_full Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
title_fullStr Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
title_full_unstemmed Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
title_sort ultrasound triggered topical delivery of bmp7 mrna for white fat browning induction via engineered smart exosomes
publisher BMC
publishDate 2021
url https://doaj.org/article/1b1e9096a06840269603bf229f1531d2
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