Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.

Primary sensory afferents of the dorsal root and trigeminal ganglia constantly transmit sensory information depicting the individual's physical and chemical environment to higher brain regions. Beyond the typical trigeminal stimuli (e.g. irritants), environmental stimuli comprise a plethora of...

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Autores principales:	Matthias Lübbert, Jessica Kyereme, Nicole Schöbel, Leopoldo Beltrán, Christian Horst Wetzel, Hanns Hatt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b23fce4693f466d9c3f31a19ab00b85
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spelling	oai:doaj.org-article:1b23fce4693f466d9c3f31a19ab00b852021-11-18T08:50:10ZTransient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.1932-620310.1371/journal.pone.0077998https://doaj.org/article/1b23fce4693f466d9c3f31a19ab00b852013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24205061/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Primary sensory afferents of the dorsal root and trigeminal ganglia constantly transmit sensory information depicting the individual's physical and chemical environment to higher brain regions. Beyond the typical trigeminal stimuli (e.g. irritants), environmental stimuli comprise a plethora of volatile chemicals with olfactory components (odorants). In spite of a complete loss of their sense of smell, anosmic patients may retain the ability to roughly discriminate between different volatile compounds. While the detailed mechanisms remain elusive, sensory structures belonging to the trigeminal system seem to be responsible for this phenomenon. In order to gain a better understanding of the mechanisms underlying the activation of the trigeminal system by volatile chemicals, we investigated odorant-induced membrane potential changes in cultured rat trigeminal neurons induced by the odorants vanillin, heliotropyl acetone, helional, and geraniol. We observed the dose-dependent depolarization of trigeminal neurons upon application of these substances occurring in a stimulus-specific manner and could show that distinct neuronal populations respond to different odorants. Using specific antagonists, we found evidence that TRPA1, TRPM8, and/or TRPV1 contribute to the activation. In order to further test this hypothesis, we used recombinantly expressed rat and human variants of these channels to investigate whether they are indeed activated by the odorants tested. We additionally found that the odorants dose-dependently inhibit two-pore potassium channels TASK1 and TASK3 heterologously expressed In Xenopus laevis oocytes. We suggest that the capability of various odorants to activate different TRP channels and to inhibit potassium channels causes neuronal depolarization and activation of distinct subpopulations of trigeminal sensory neurons, forming the basis for a specific representation of volatile chemicals in the trigeminal ganglia.Matthias LübbertJessica KyeremeNicole SchöbelLeopoldo BeltránChristian Horst WetzelHanns HattPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77998 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias Lübbert Jessica Kyereme Nicole Schöbel Leopoldo Beltrán Christian Horst Wetzel Hanns Hatt Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
description	Primary sensory afferents of the dorsal root and trigeminal ganglia constantly transmit sensory information depicting the individual's physical and chemical environment to higher brain regions. Beyond the typical trigeminal stimuli (e.g. irritants), environmental stimuli comprise a plethora of volatile chemicals with olfactory components (odorants). In spite of a complete loss of their sense of smell, anosmic patients may retain the ability to roughly discriminate between different volatile compounds. While the detailed mechanisms remain elusive, sensory structures belonging to the trigeminal system seem to be responsible for this phenomenon. In order to gain a better understanding of the mechanisms underlying the activation of the trigeminal system by volatile chemicals, we investigated odorant-induced membrane potential changes in cultured rat trigeminal neurons induced by the odorants vanillin, heliotropyl acetone, helional, and geraniol. We observed the dose-dependent depolarization of trigeminal neurons upon application of these substances occurring in a stimulus-specific manner and could show that distinct neuronal populations respond to different odorants. Using specific antagonists, we found evidence that TRPA1, TRPM8, and/or TRPV1 contribute to the activation. In order to further test this hypothesis, we used recombinantly expressed rat and human variants of these channels to investigate whether they are indeed activated by the odorants tested. We additionally found that the odorants dose-dependently inhibit two-pore potassium channels TASK1 and TASK3 heterologously expressed In Xenopus laevis oocytes. We suggest that the capability of various odorants to activate different TRP channels and to inhibit potassium channels causes neuronal depolarization and activation of distinct subpopulations of trigeminal sensory neurons, forming the basis for a specific representation of volatile chemicals in the trigeminal ganglia.
format	article
author	Matthias Lübbert Jessica Kyereme Nicole Schöbel Leopoldo Beltrán Christian Horst Wetzel Hanns Hatt
author_facet	Matthias Lübbert Jessica Kyereme Nicole Schöbel Leopoldo Beltrán Christian Horst Wetzel Hanns Hatt
author_sort	Matthias Lübbert
title	Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
title_short	Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
title_full	Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
title_fullStr	Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
title_full_unstemmed	Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
title_sort	transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1b23fce4693f466d9c3f31a19ab00b85
work_keys_str_mv	AT matthiaslubbert transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons AT jessicakyereme transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons AT nicoleschobel transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons AT leopoldobeltran transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons AT christianhorstwetzel transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons AT hannshatt transientreceptorpotentialchannelsencodevolatilechemicalssensedbyrattrigeminalganglionneurons
_version_	1718421248613023744

Transient receptor potential channels encode volatile chemicals sensed by rat trigeminal ganglion neurons.

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