Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.

Metabolic diseases and obesity are developing worldwide in a context of plethoric intake of high energy diets. The intestine may play a pivotal role due to diet-induced alterations in microbiota composition and increased permeability to bacterial lipopolysaccharide inducing metabolic inflammation. E...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie-Edith Arnal, Jing Zhang, Stefano Messori, Paolo Bosi, Hauke Smidt, Jean-Paul Lallès
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b2535652c064da68c78ba22d217aad9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b2535652c064da68c78ba22d217aad9
record_format	dspace
spelling	oai:doaj.org-article:1b2535652c064da68c78ba22d217aad92021-11-18T08:33:54ZEarly changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.1932-620310.1371/journal.pone.0087967https://doaj.org/article/1b2535652c064da68c78ba22d217aad92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505340/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Metabolic diseases and obesity are developing worldwide in a context of plethoric intake of high energy diets. The intestine may play a pivotal role due to diet-induced alterations in microbiota composition and increased permeability to bacterial lipopolysaccharide inducing metabolic inflammation. Early programming of metabolic disorders appearing in later life is also suspected, but data on the intestine are lacking. Therefore, we hypothesized that early disturbances in microbial colonization have short- and long-lasting consequences on selected intestinal components including key digestive enzymes and protective inducible heat shock proteins (HSP). The hypothesis was tested in swine offspring born to control mothers (n = 12) or mothers treated with the antibiotic amoxicillin around parturition (n = 11), and slaughtered serially at 14, 28 and 42 days of age to assess short-term effects. To evaluate long-term consequences, young adult offspring from the same litters were offered a normal or a fat-enriched diet for 4 weeks between 140 and 169 days of age and were then slaughtered. Amoxicillin treatment transiently modified both mother and offspring microbiota. This was associated with early but transient reduction in ileal alkaline phosphatase, HSP70 (but not HSP27) and crypt depth, suggesting a milder or delayed intestinal response to bacteria in offspring born to antibiotic-treated mothers. More importantly, we disclosed long-term consequences of this treatment on jejunal alkaline phosphatase (reduced) and jejunal and ileal dipeptidylpeptidase IV (increased and decreased, respectively) of offspring born to antibiotic-treated dams. Significant interactions between early antibiotic treatment and later diet were observed for jejunal alkaline phosphatase and sucrase. By contrast, inducible HSPs were not affected. In conclusion, our data suggest that early changes in bacterial colonization not only modulate intestinal architecture and function transiently, but also exert site- and sometimes diet-specific long-term effects on key components of intestinal homeostasis.Marie-Edith ArnalJing ZhangStefano MessoriPaolo BosiHauke SmidtJean-Paul LallèsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87967 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Edith Arnal Jing Zhang Stefano Messori Paolo Bosi Hauke Smidt Jean-Paul Lallès Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
description	Metabolic diseases and obesity are developing worldwide in a context of plethoric intake of high energy diets. The intestine may play a pivotal role due to diet-induced alterations in microbiota composition and increased permeability to bacterial lipopolysaccharide inducing metabolic inflammation. Early programming of metabolic disorders appearing in later life is also suspected, but data on the intestine are lacking. Therefore, we hypothesized that early disturbances in microbial colonization have short- and long-lasting consequences on selected intestinal components including key digestive enzymes and protective inducible heat shock proteins (HSP). The hypothesis was tested in swine offspring born to control mothers (n = 12) or mothers treated with the antibiotic amoxicillin around parturition (n = 11), and slaughtered serially at 14, 28 and 42 days of age to assess short-term effects. To evaluate long-term consequences, young adult offspring from the same litters were offered a normal or a fat-enriched diet for 4 weeks between 140 and 169 days of age and were then slaughtered. Amoxicillin treatment transiently modified both mother and offspring microbiota. This was associated with early but transient reduction in ileal alkaline phosphatase, HSP70 (but not HSP27) and crypt depth, suggesting a milder or delayed intestinal response to bacteria in offspring born to antibiotic-treated mothers. More importantly, we disclosed long-term consequences of this treatment on jejunal alkaline phosphatase (reduced) and jejunal and ileal dipeptidylpeptidase IV (increased and decreased, respectively) of offspring born to antibiotic-treated dams. Significant interactions between early antibiotic treatment and later diet were observed for jejunal alkaline phosphatase and sucrase. By contrast, inducible HSPs were not affected. In conclusion, our data suggest that early changes in bacterial colonization not only modulate intestinal architecture and function transiently, but also exert site- and sometimes diet-specific long-term effects on key components of intestinal homeostasis.
format	article
author	Marie-Edith Arnal Jing Zhang Stefano Messori Paolo Bosi Hauke Smidt Jean-Paul Lallès
author_facet	Marie-Edith Arnal Jing Zhang Stefano Messori Paolo Bosi Hauke Smidt Jean-Paul Lallès
author_sort	Marie-Edith Arnal
title	Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
title_short	Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
title_full	Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
title_fullStr	Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
title_full_unstemmed	Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.
title_sort	early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult swine.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1b2535652c064da68c78ba22d217aad9
work_keys_str_mv	AT marieeditharnal earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine AT jingzhang earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine AT stefanomessori earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine AT paolobosi earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine AT haukesmidt earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine AT jeanpaullalles earlychangesinmicrobialcolonizationselectivelymodulateintestinalenzymesbutnotinducibleheatshockproteinsinyoungadultswine
_version_	1718421606088310784

Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult Swine.

Ejemplares similares