Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.

Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ellen Ruth A Morris, Ashley G Boyle, Miia Riihimäki, Anna Aspán, Eman Anis, Andrew E Hillhouse, Ivan Ivanov, Angela I Bordin, John Pringle, Noah D Cohen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b2d436b7801477dafe2dfd35f0eff5d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1b2d436b7801477dafe2dfd35f0eff5d
record_format	dspace
spelling	oai:doaj.org-article:1b2d436b7801477dafe2dfd35f0eff5d2021-12-02T20:10:42ZDifferences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.1932-620310.1371/journal.pone.0252804https://doaj.org/article/1b2d436b7801477dafe2dfd35f0eff5d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252804https://doaj.org/toc/1932-6203Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor infects other host mammals or vectors, and infection results in short-lived immunity. Whether pathogen factors enable SEE to remain in horses without causing clinical signs remains poorly understood. Thus, our objective was to use next-generation sequencing technologies to characterize the genome, methylome, and transcriptome of isolates of SEE from horses with acute clinical strangles and inapparent carrier horses-including isolates recovered from individual horses sampled repeatedly-to assess pathogen-associated changes that might reflect specific adaptions of SEE to the host that contribute to inapparent carriage. The accessory genome elements and methylome of SEE isolates from Sweden and Pennsylvania revealed no significant or consistent differences between acute clinical and inapparent carrier isolates of SEE. RNA sequencing of SEE isolates from Pennsylvania demonstrated no genes that were differentially expressed between acute clinical and inapparent carrier isolates of SEE. The absence of specific, consistent changes in the accessory genomes, methylomes, and transcriptomes of acute clinical and inapparent carrier isolates of SEE indicates that adaptations of SEE to the host are unlikely to explain the carrier state of SEE. Efforts to understand the carrier state of SEE should instead focus on host factors.Ellen Ruth A MorrisAshley G BoyleMiia RiihimäkiAnna AspánEman AnisAndrew E HillhouseIvan IvanovAngela I BordinJohn PringleNoah D CohenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252804 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ellen Ruth A Morris Ashley G Boyle Miia Riihimäki Anna Aspán Eman Anis Andrew E Hillhouse Ivan Ivanov Angela I Bordin John Pringle Noah D Cohen Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
description	Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor infects other host mammals or vectors, and infection results in short-lived immunity. Whether pathogen factors enable SEE to remain in horses without causing clinical signs remains poorly understood. Thus, our objective was to use next-generation sequencing technologies to characterize the genome, methylome, and transcriptome of isolates of SEE from horses with acute clinical strangles and inapparent carrier horses-including isolates recovered from individual horses sampled repeatedly-to assess pathogen-associated changes that might reflect specific adaptions of SEE to the host that contribute to inapparent carriage. The accessory genome elements and methylome of SEE isolates from Sweden and Pennsylvania revealed no significant or consistent differences between acute clinical and inapparent carrier isolates of SEE. RNA sequencing of SEE isolates from Pennsylvania demonstrated no genes that were differentially expressed between acute clinical and inapparent carrier isolates of SEE. The absence of specific, consistent changes in the accessory genomes, methylomes, and transcriptomes of acute clinical and inapparent carrier isolates of SEE indicates that adaptations of SEE to the host are unlikely to explain the carrier state of SEE. Efforts to understand the carrier state of SEE should instead focus on host factors.
format	article
author	Ellen Ruth A Morris Ashley G Boyle Miia Riihimäki Anna Aspán Eman Anis Andrew E Hillhouse Ivan Ivanov Angela I Bordin John Pringle Noah D Cohen
author_facet	Ellen Ruth A Morris Ashley G Boyle Miia Riihimäki Anna Aspán Eman Anis Andrew E Hillhouse Ivan Ivanov Angela I Bordin John Pringle Noah D Cohen
author_sort	Ellen Ruth A Morris
title	Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
title_short	Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
title_full	Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
title_fullStr	Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
title_full_unstemmed	Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
title_sort	differences in the genome, methylome, and transcriptome do not differentiate isolates of streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1b2d436b7801477dafe2dfd35f0eff5d
work_keys_str_mv	AT ellenruthamorris differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT ashleygboyle differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT miiariihimaki differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT annaaspan differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT emananis differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT andrewehillhouse differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT ivanivanov differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT angelaibordin differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT johnpringle differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers AT noahdcohen differencesinthegenomemethylomeandtranscriptomedonotdifferentiateisolatesofstreptococcusequisubspequifromhorseswithacuteclinicalsignsfromisolatesofinapparentcarriers
_version_	1718374930344574976

Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.

Ejemplares similares