Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions

In this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of n...

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Autores principales:	Ebru Bodur, A. Neşe Çokuğraş
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2006
Materias:	Benzoylcholine butyrylcholinesterase indole-acetic acid inhibition Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/1b3cbd9d771b457d90d83d6c37a43fa7
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id	oai:doaj.org-article:1b3cbd9d771b457d90d83d6c37a43fa7
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spelling	oai:doaj.org-article:1b3cbd9d771b457d90d83d6c37a43fa72021-12-02T10:49:56ZBenzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions0250-46851303-829Xhttps://doaj.org/article/1b3cbd9d771b457d90d83d6c37a43fa72006-12-01T00:00:00Zhttp://www.turkjbiochem.com/2006/182.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XIn this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of nH = 0.95, displaying that the enzyme follows Michaelis-Menten kinetics with regard to benzoylcholine. Through inhibition studies, indole-3-acetic acid was found to be a noncompetitive inhibitor for human serum butyrylcholinesterase with benzoylcholineas substrate. The Ki value was calculated as 1.86 ± 0.27 mM. In our previous study on human serum butyrylcholinesterase using butyrylthiocholine as substrate, indole-3-acetic acid was found to be a linear-mixed type inhibitor.This difference in kinetic behavior with regards to two different substrates could arise from the tighter binding of the more bulky and hydrophobic benzoyl group to the hydrophobic pocket of the active site gorge rather than the butyryl group and that the binding of this butyryl group could be sterically hindered by the indole-3-acetic acid.Ebru Bodur,A. Neşe ÇokuğraşDe GruyterarticleBenzoylcholinebutyrylcholinesteraseindole-acetic acidinhibitionBiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 31, Iss 4, Pp 182-186 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	Benzoylcholine butyrylcholinesterase indole-acetic acid inhibition Biochemistry QD415-436
spellingShingle	Benzoylcholine butyrylcholinesterase indole-acetic acid inhibition Biochemistry QD415-436 Ebru Bodur, A. Neşe Çokuğraş Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
description	In this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of nH = 0.95, displaying that the enzyme follows Michaelis-Menten kinetics with regard to benzoylcholine. Through inhibition studies, indole-3-acetic acid was found to be a noncompetitive inhibitor for human serum butyrylcholinesterase with benzoylcholineas substrate. The Ki value was calculated as 1.86 ± 0.27 mM. In our previous study on human serum butyrylcholinesterase using butyrylthiocholine as substrate, indole-3-acetic acid was found to be a linear-mixed type inhibitor.This difference in kinetic behavior with regards to two different substrates could arise from the tighter binding of the more bulky and hydrophobic benzoyl group to the hydrophobic pocket of the active site gorge rather than the butyryl group and that the binding of this butyryl group could be sterically hindered by the indole-3-acetic acid.
format	article
author	Ebru Bodur, A. Neşe Çokuğraş
author_facet	Ebru Bodur, A. Neşe Çokuğraş
author_sort	Ebru Bodur,
title	Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
title_short	Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
title_full	Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
title_fullStr	Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
title_full_unstemmed	Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
title_sort	benzoylcholine, indole-3-acetic and human serum butyrylcholinesterase interactions
publisher	De Gruyter
publishDate	2006
url	https://doaj.org/article/1b3cbd9d771b457d90d83d6c37a43fa7
work_keys_str_mv	AT ebrubodur benzoylcholineindole3aceticandhumanserumbutyrylcholinesteraseinteractions AT anesecokugras benzoylcholineindole3aceticandhumanserumbutyrylcholinesteraseinteractions
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Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions

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