Autophagy in human embryonic stem cells.

Autophagy (macroautophagy) is a degradative process that involves the sequestration of cytosolic material including organelles into double membrane vesicles termed autophagosomes for delivery to the lysosome. Autophagy is essential for preimplantation development of mouse embryos and cavitation of e...

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Autores principales:	Thien Tra, Lan Gong, Lin-Pin Kao, Xue-Lei Li, Catarina Grandela, Rodney J Devenish, Ernst Wolvetang, Mark Prescott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b43bf42ce5148bfb27237f7a594e527
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spelling	oai:doaj.org-article:1b43bf42ce5148bfb27237f7a594e5272021-11-18T07:34:23ZAutophagy in human embryonic stem cells.1932-620310.1371/journal.pone.0027485https://doaj.org/article/1b43bf42ce5148bfb27237f7a594e5272011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22110659/?tool=EBIhttps://doaj.org/toc/1932-6203Autophagy (macroautophagy) is a degradative process that involves the sequestration of cytosolic material including organelles into double membrane vesicles termed autophagosomes for delivery to the lysosome. Autophagy is essential for preimplantation development of mouse embryos and cavitation of embryoid bodies. The precise roles of autophagy during early human embryonic development, remain however largely uncharacterized. Since human embryonic stem cells constitute a unique model system to study early human embryogenesis we investigated the occurrence of autophagy in human embryonic stem cells. We have, using lentiviral transduction, established multiple human embryonic stem cell lines that stably express GFP-LC3, a fluorescent marker for the autophagosome. Each cell line displays both a normal karyotype and pluripotency as indicated by the presence of cell types representative of the three germlayers in derived teratomas. GFP expression and labelling of autophagosomes is retained after differentiation. Baseline levels of autophagy detected in cultured undifferentiated hESC were increased or decreased in the presence of rapamycin and wortmannin, respectively. Interestingly, autophagy was upregulated in hESCs induced to undergo differentiation by treatment with type I TGF-beta receptor inhibitor SB431542 or removal of MEF secreted maintenance factors. In conclusion we have established hESCs capable of reporting macroautophagy and identify a novel link between autophagy and early differentiation events in hESC.Thien TraLan GongLin-Pin KaoXue-Lei LiCatarina GrandelaRodney J DevenishErnst WolvetangMark PrescottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27485 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thien Tra Lan Gong Lin-Pin Kao Xue-Lei Li Catarina Grandela Rodney J Devenish Ernst Wolvetang Mark Prescott Autophagy in human embryonic stem cells.
description	Autophagy (macroautophagy) is a degradative process that involves the sequestration of cytosolic material including organelles into double membrane vesicles termed autophagosomes for delivery to the lysosome. Autophagy is essential for preimplantation development of mouse embryos and cavitation of embryoid bodies. The precise roles of autophagy during early human embryonic development, remain however largely uncharacterized. Since human embryonic stem cells constitute a unique model system to study early human embryogenesis we investigated the occurrence of autophagy in human embryonic stem cells. We have, using lentiviral transduction, established multiple human embryonic stem cell lines that stably express GFP-LC3, a fluorescent marker for the autophagosome. Each cell line displays both a normal karyotype and pluripotency as indicated by the presence of cell types representative of the three germlayers in derived teratomas. GFP expression and labelling of autophagosomes is retained after differentiation. Baseline levels of autophagy detected in cultured undifferentiated hESC were increased or decreased in the presence of rapamycin and wortmannin, respectively. Interestingly, autophagy was upregulated in hESCs induced to undergo differentiation by treatment with type I TGF-beta receptor inhibitor SB431542 or removal of MEF secreted maintenance factors. In conclusion we have established hESCs capable of reporting macroautophagy and identify a novel link between autophagy and early differentiation events in hESC.
format	article
author	Thien Tra Lan Gong Lin-Pin Kao Xue-Lei Li Catarina Grandela Rodney J Devenish Ernst Wolvetang Mark Prescott
author_facet	Thien Tra Lan Gong Lin-Pin Kao Xue-Lei Li Catarina Grandela Rodney J Devenish Ernst Wolvetang Mark Prescott
author_sort	Thien Tra
title	Autophagy in human embryonic stem cells.
title_short	Autophagy in human embryonic stem cells.
title_full	Autophagy in human embryonic stem cells.
title_fullStr	Autophagy in human embryonic stem cells.
title_full_unstemmed	Autophagy in human embryonic stem cells.
title_sort	autophagy in human embryonic stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1b43bf42ce5148bfb27237f7a594e527
work_keys_str_mv	AT thientra autophagyinhumanembryonicstemcells AT langong autophagyinhumanembryonicstemcells AT linpinkao autophagyinhumanembryonicstemcells AT xueleili autophagyinhumanembryonicstemcells AT catarinagrandela autophagyinhumanembryonicstemcells AT rodneyjdevenish autophagyinhumanembryonicstemcells AT ernstwolvetang autophagyinhumanembryonicstemcells AT markprescott autophagyinhumanembryonicstemcells
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Autophagy in human embryonic stem cells.

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