Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health

The extensive use of antibiotics has caused antimicrobial resistance and multidrug resistance in Escherichia coli and gradual expands it into a worldwide problem. The resistant E. coli could be transmitted to humans through animal products, thereby creating a problem for bacterial treatment in human...

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Autores principales:	Wei Wang, Lanping Yu, Wenwen Hao, Fusen Zhang, Meijie Jiang, Shuping Zhao, Fangkun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Escherichia coli analysis of drug resistance drug-resistant phenotype drug-resistant genotype multi-locus sequence typing Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/1b46d2392fb84bd9aae4829344bc47b7
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spelling	oai:doaj.org-article:1b46d2392fb84bd9aae4829344bc47b72021-12-02T07:38:27ZMulti-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health2296-256510.3389/fpubh.2021.780700https://doaj.org/article/1b46d2392fb84bd9aae4829344bc47b72021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpubh.2021.780700/fullhttps://doaj.org/toc/2296-2565The extensive use of antibiotics has caused antimicrobial resistance and multidrug resistance in Escherichia coli and gradual expands it into a worldwide problem. The resistant E. coli could be transmitted to humans through animal products, thereby creating a problem for bacterial treatment in humans and resulting in a public health issue. This study aims to investigate the molecular typing and drug resistance of swine and human origin E. coli within the same prefecture-level cities of Shandong Province and the potential risk of E. coli on public health. The drug sensitivity results indicated that tetracycline (TE) (97.17%) is a major antibiotic with high drug resistance in 106 swine origin E. coli. There was a significant difference in the drug-resistant genotypes between the two sources, of which the blaTEM positive rate was the highest in the genera of β-lactams (99% in swines and 100% in humans). Among the 146 E. coli isolates, 98 (91.51% swine origin) and 31 (77.5% human origin) isolates were simultaneously resistant to three or more classes of antibiotics, respectively. The multi-locus sequence typing (MLST) results indicate that the 106 swine origin E. coli isolates are divided into 25 STs with ST1258, ST361, and ST10 being the dominant sequence analysis typing strains. There were 19 MLST genotypes in 40 strains of human E. coli from Tai'an, Shandong Province, with ST1193, ST73, ST648, ST131, ST10, and ST1668 being the dominant strains. Moreover, the cluster analysis showed that CCl0 and CC23 were the common clonal complexes (CCs) from the two sources. Our results provide a theoretical basis for guiding the rational use of antibiotics and preventing the spread of drug-resistant bacteria, and also provide epidemiological data for the risk analysis of foodborne bacteria and antimicrobial resistance in swine farms in Shandong Province.Wei WangLanping YuWenwen HaoFusen ZhangMeijie JiangShuping ZhaoFangkun WangFrontiers Media S.A.articleEscherichia colianalysis of drug resistancedrug-resistant phenotypedrug-resistant genotypemulti-locus sequence typingPublic aspects of medicineRA1-1270ENFrontiers in Public Health, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli analysis of drug resistance drug-resistant phenotype drug-resistant genotype multi-locus sequence typing Public aspects of medicine RA1-1270
spellingShingle	Escherichia coli analysis of drug resistance drug-resistant phenotype drug-resistant genotype multi-locus sequence typing Public aspects of medicine RA1-1270 Wei Wang Lanping Yu Wenwen Hao Fusen Zhang Meijie Jiang Shuping Zhao Fangkun Wang Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
description	The extensive use of antibiotics has caused antimicrobial resistance and multidrug resistance in Escherichia coli and gradual expands it into a worldwide problem. The resistant E. coli could be transmitted to humans through animal products, thereby creating a problem for bacterial treatment in humans and resulting in a public health issue. This study aims to investigate the molecular typing and drug resistance of swine and human origin E. coli within the same prefecture-level cities of Shandong Province and the potential risk of E. coli on public health. The drug sensitivity results indicated that tetracycline (TE) (97.17%) is a major antibiotic with high drug resistance in 106 swine origin E. coli. There was a significant difference in the drug-resistant genotypes between the two sources, of which the blaTEM positive rate was the highest in the genera of β-lactams (99% in swines and 100% in humans). Among the 146 E. coli isolates, 98 (91.51% swine origin) and 31 (77.5% human origin) isolates were simultaneously resistant to three or more classes of antibiotics, respectively. The multi-locus sequence typing (MLST) results indicate that the 106 swine origin E. coli isolates are divided into 25 STs with ST1258, ST361, and ST10 being the dominant sequence analysis typing strains. There were 19 MLST genotypes in 40 strains of human E. coli from Tai'an, Shandong Province, with ST1193, ST73, ST648, ST131, ST10, and ST1668 being the dominant strains. Moreover, the cluster analysis showed that CCl0 and CC23 were the common clonal complexes (CCs) from the two sources. Our results provide a theoretical basis for guiding the rational use of antibiotics and preventing the spread of drug-resistant bacteria, and also provide epidemiological data for the risk analysis of foodborne bacteria and antimicrobial resistance in swine farms in Shandong Province.
format	article
author	Wei Wang Lanping Yu Wenwen Hao Fusen Zhang Meijie Jiang Shuping Zhao Fangkun Wang
author_facet	Wei Wang Lanping Yu Wenwen Hao Fusen Zhang Meijie Jiang Shuping Zhao Fangkun Wang
author_sort	Wei Wang
title	Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
title_short	Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
title_full	Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
title_fullStr	Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
title_full_unstemmed	Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health
title_sort	multi-locus sequence typing and drug resistance analysis of swine origin escherichia coli in shandong of china and its potential risk on public health
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1b46d2392fb84bd9aae4829344bc47b7
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Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health

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