Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells

Abstract Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, th...

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Autores principales:	Nour M. AlSawaftah, Nahid S. Awad, Vinod Paul, Paul S. Kawak, Mohammad H. Al-Sayah, Ghaleb A. Husseini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1b4769fef82e42cfba6870a5f9082be9
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spelling	oai:doaj.org-article:1b4769fef82e42cfba6870a5f9082be92021-12-02T15:02:31ZTransferrin-modified liposomes triggered with ultrasound to treat HeLa cells10.1038/s41598-021-90349-62045-2322https://doaj.org/article/1b4769fef82e42cfba6870a5f9082be92021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90349-6https://doaj.org/toc/2045-2322Abstract Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, these liposomes can be triggered to release their content in a controlled manner using ultrasound (US). In this study, drug release from pegylated calcein-loaded liposomes modified with transferrin (Tf) and triggered with US was evaluated. Low-frequency ultrasound at 20-kHz using three different power densities (6.2 mW/cm2, 9 mW/cm2 and 10 mW/cm2) was found to increase calcein release. In addition, transferrin-conjugated pegylated liposomes (Tf-PEG liposomes) were found to be more sonosensitive compared to the non-targeted (control) liposomes. Calcein uptake by HeLa cells was found to be significantly higher with the Tf-PEG liposomes compared to the non-targeted control liposomes. This uptake was further enhanced following the exposure to low-frequency ultrasound (at 35 kHz). These findings show that targeted liposomes triggered with US have promising potential as a safe and effective drug delivery platform.Nour M. AlSawaftahNahid S. AwadVinod PaulPaul S. KawakMohammad H. Al-SayahGhaleb A. HusseiniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nour M. AlSawaftah Nahid S. Awad Vinod Paul Paul S. Kawak Mohammad H. Al-Sayah Ghaleb A. Husseini Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
description	Abstract Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery to reduce undesirable side effects while enhancing the efficiency of the encapsulated therapeutics. Upon reaching the tumor site, these liposomes can be triggered to release their content in a controlled manner using ultrasound (US). In this study, drug release from pegylated calcein-loaded liposomes modified with transferrin (Tf) and triggered with US was evaluated. Low-frequency ultrasound at 20-kHz using three different power densities (6.2 mW/cm2, 9 mW/cm2 and 10 mW/cm2) was found to increase calcein release. In addition, transferrin-conjugated pegylated liposomes (Tf-PEG liposomes) were found to be more sonosensitive compared to the non-targeted (control) liposomes. Calcein uptake by HeLa cells was found to be significantly higher with the Tf-PEG liposomes compared to the non-targeted control liposomes. This uptake was further enhanced following the exposure to low-frequency ultrasound (at 35 kHz). These findings show that targeted liposomes triggered with US have promising potential as a safe and effective drug delivery platform.
format	article
author	Nour M. AlSawaftah Nahid S. Awad Vinod Paul Paul S. Kawak Mohammad H. Al-Sayah Ghaleb A. Husseini
author_facet	Nour M. AlSawaftah Nahid S. Awad Vinod Paul Paul S. Kawak Mohammad H. Al-Sayah Ghaleb A. Husseini
author_sort	Nour M. AlSawaftah
title	Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
title_short	Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
title_full	Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
title_fullStr	Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
title_full_unstemmed	Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells
title_sort	transferrin-modified liposomes triggered with ultrasound to treat hela cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1b4769fef82e42cfba6870a5f9082be9
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Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells

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