Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total ba...

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Autor principal:	Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño Dolly
Formato:	article
Lenguaje:	EN ES
Publicado:	Pontificia Universidad Javeriana 2020
Materias:	epiphytic bacteria; lipases; pcr; thiolases; ulva lactuca. Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/1b4b40d9736a4faca8c63bb122e99d55
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spelling	oai:doaj.org-article:1b4b40d9736a4faca8c63bb122e99d552021-11-16T20:16:09ZTwo possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation10.11144/Javeriana.SC25-2.tpce0122-74832027-1352https://doaj.org/article/1b4b40d9736a4faca8c63bb122e99d552020-07-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/25583https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total bacterial DNA isolation from the surface of U. lactuca thalli. Then, we designed sets of primers and used them directly for PCR amplification. The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. We obtained high molecular weight and good quality total bacterial DNA that served as a template to identify a fragment corresponding to an Acetyl-CoA C-Acetyltransferase (or Thiolase), and a candidate fragment for a versatile “true” lipase. We expressed the possible “true” lipase gene fragment heterologously in Escherichia coli and obtained proof of hydrolytic activity on Tributyrin, Tween-20, and Olive-oil media. This study resulted in new knowledge on U. lactuca-associated epiphytic bacteria as possible brand-new sources of enzymes such as thiolases and “true” lipases. However, future studies are required to describe the characteristics and important applications of these candidate enzymes.Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño DollyPontificia Universidad Javerianaarticleepiphytic bacteria; lipases; pcr; thiolases; ulva lactuca.Science (General)Q1-390ENESUniversitas Scientiarum, Vol 25, Iss 2, Pp 247-275 (2020)
institution	DOAJ
collection	DOAJ
language	EN ES
topic	epiphytic bacteria; lipases; pcr; thiolases; ulva lactuca. Science (General) Q1-390
spellingShingle	epiphytic bacteria; lipases; pcr; thiolases; ulva lactuca. Science (General) Q1-390 Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño Dolly Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
description	Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total bacterial DNA isolation from the surface of U. lactuca thalli. Then, we designed sets of primers and used them directly for PCR amplification. The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. We obtained high molecular weight and good quality total bacterial DNA that served as a template to identify a fragment corresponding to an Acetyl-CoA C-Acetyltransferase (or Thiolase), and a candidate fragment for a versatile “true” lipase. We expressed the possible “true” lipase gene fragment heterologously in Escherichia coli and obtained proof of hydrolytic activity on Tributyrin, Tween-20, and Olive-oil media. This study resulted in new knowledge on U. lactuca-associated epiphytic bacteria as possible brand-new sources of enzymes such as thiolases and “true” lipases. However, future studies are required to describe the characteristics and important applications of these candidate enzymes.
format	article
author	Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño Dolly
author_facet	Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño Dolly
author_sort	Ruiz-Toquica Jordan Steven, Comba-González Natalia Beatriz, Montoya-Castaño Dolly
title	Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
title_short	Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
title_full	Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
title_fullStr	Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
title_full_unstemmed	Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
title_sort	two possible candidate enzymes from ulva lactuca-associated epiphytic bacteria obtained through pcr and functional evaluation
publisher	Pontificia Universidad Javeriana
publishDate	2020
url	https://doaj.org/article/1b4b40d9736a4faca8c63bb122e99d55
work_keys_str_mv	AT ruiztoquicajordanstevencombagonzaleznataliabeatrizmontoyacastanodolly twopossiblecandidateenzymesfromulvalactucaassociatedepiphyticbacteriaobtainedthroughpcrandfunctionalevaluation
_version_	1718426033674256384

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

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