Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator

Elicitors are biochemicals, and the cell wall-derived elicitors from fungi can trigger defence mechanisms in plants by increasing the phytoalexin accumulation when they encounter the pathogens. The main objective of this research was to purify and characterize a cell wall elicitor from Trichoderma a...

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Autores principales:	Savitha De Britto, Shreya M. Joshi, Sudisha Jogaiah
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	T. atroviride Trehalose-cell wall elicitor Induced systemic resistance (ISR) and plant growth regulator Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1b4d0545310f49b1a7243fc9ddb4ab71
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spelling	oai:doaj.org-article:1b4d0545310f49b1a7243fc9ddb4ab712021-11-28T04:32:33ZTrehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator2215-017X10.1016/j.btre.2021.e00690https://doaj.org/article/1b4d0545310f49b1a7243fc9ddb4ab712021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215017X21001065https://doaj.org/toc/2215-017XElicitors are biochemicals, and the cell wall-derived elicitors from fungi can trigger defence mechanisms in plants by increasing the phytoalexin accumulation when they encounter the pathogens. The main objective of this research was to purify and characterize a cell wall elicitor from Trichoderma atroviride (TaCWE) and evaluate the seed priming effect of that elicitor for inducing systemic resistance in broccoli plants against leaf spot disease. Amongst the tested TaCWE concentrations of the seed priming (5, 10, & 25 mg ml−1), 10.0 mg ml−1 showed significantly (P < 0.05) improved early emergence, the rate of germination at 94%, and observed seedling vigour of 2601. Also, elicitor (10 mg ml−1) treatment alone induced 57% plant protection. On the contrary, the elicitor treated and pathogen inoculated plants induced a notable 72% protection against leaf spot disease of broccoli caused by A. brassicicola. Thus, the primed seeds with elicitor showed induced disease resistance and plant growth promotion. The prominent molecule present in the purified extracted cell wall elicitor is confirmed as trehalose. The AFM analysis indicated the trehalose length and width as 10.16 µm and 2.148 µm, respectively. FTIR chromatogram further confirmed trehalose in abundance with traces of carbon, hydrogen, nitrogen, oxygen, and LC-MS profile with a single peak eluted with a retention time of 3.78 min. The findings of this study contribute to understanding better the role of trehalose, a biogenic cell-wall elicitor that can induce systemic resistance against leaf spot disease and regulate plant growth in the broccoli plants.Savitha De BrittoShreya M. JoshiSudisha JogaiahElsevierarticleT. atrovirideTrehalose-cell wall elicitorInduced systemic resistance (ISR)and plant growth regulatorBiotechnologyTP248.13-248.65ENBiotechnology Reports, Vol 32, Iss , Pp e00690- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	T. atroviride Trehalose-cell wall elicitor Induced systemic resistance (ISR) and plant growth regulator Biotechnology TP248.13-248.65
spellingShingle	T. atroviride Trehalose-cell wall elicitor Induced systemic resistance (ISR) and plant growth regulator Biotechnology TP248.13-248.65 Savitha De Britto Shreya M. Joshi Sudisha Jogaiah Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
description	Elicitors are biochemicals, and the cell wall-derived elicitors from fungi can trigger defence mechanisms in plants by increasing the phytoalexin accumulation when they encounter the pathogens. The main objective of this research was to purify and characterize a cell wall elicitor from Trichoderma atroviride (TaCWE) and evaluate the seed priming effect of that elicitor for inducing systemic resistance in broccoli plants against leaf spot disease. Amongst the tested TaCWE concentrations of the seed priming (5, 10, & 25 mg ml−1), 10.0 mg ml−1 showed significantly (P < 0.05) improved early emergence, the rate of germination at 94%, and observed seedling vigour of 2601. Also, elicitor (10 mg ml−1) treatment alone induced 57% plant protection. On the contrary, the elicitor treated and pathogen inoculated plants induced a notable 72% protection against leaf spot disease of broccoli caused by A. brassicicola. Thus, the primed seeds with elicitor showed induced disease resistance and plant growth promotion. The prominent molecule present in the purified extracted cell wall elicitor is confirmed as trehalose. The AFM analysis indicated the trehalose length and width as 10.16 µm and 2.148 µm, respectively. FTIR chromatogram further confirmed trehalose in abundance with traces of carbon, hydrogen, nitrogen, oxygen, and LC-MS profile with a single peak eluted with a retention time of 3.78 min. The findings of this study contribute to understanding better the role of trehalose, a biogenic cell-wall elicitor that can induce systemic resistance against leaf spot disease and regulate plant growth in the broccoli plants.
format	article
author	Savitha De Britto Shreya M. Joshi Sudisha Jogaiah
author_facet	Savitha De Britto Shreya M. Joshi Sudisha Jogaiah
author_sort	Savitha De Britto
title	Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
title_short	Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
title_full	Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
title_fullStr	Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
title_full_unstemmed	Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
title_sort	trehalose: a mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1b4d0545310f49b1a7243fc9ddb4ab71
work_keys_str_mv	AT savithadebritto trehaloseamycogeniccellwallelicitorelicitresistanceagainstleafspotdiseaseofbroccoliandactsasaplantgrowthregulator AT shreyamjoshi trehaloseamycogeniccellwallelicitorelicitresistanceagainstleafspotdiseaseofbroccoliandactsasaplantgrowthregulator AT sudishajogaiah trehaloseamycogeniccellwallelicitorelicitresistanceagainstleafspotdiseaseofbroccoliandactsasaplantgrowthregulator
_version_	1718408303834300416

Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator

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